LOCUS       sequence-359491-        1913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing J23101 anderson promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_043
FEATURES             Location/Qualifiers
     source          1..1913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     423..442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(661..765)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      868..1456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1357..1376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1571..1605
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             complement(join(1713..1913,1..591,592..660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
       61 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
      121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
      181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
      241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
      301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
      361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
      421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
      481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
      541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
      601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
      661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
      721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      781 agtgccacct gtcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      841 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      901 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      961 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1021 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1081 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1141 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1201 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1261 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1321 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1381 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1441 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1501 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtagtcg
     1561 gtctctgacc tttacagcta gctcagtcct aggtattatg ctagctggct gagaccaaga
     1621 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1681 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1741 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1801 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgggaccca
     1861 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cga
//
