LOCUS       sequence-359512-        1936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector containing L3S2P24 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Goosens VJ, Walker KT, Aragon SM, Singh A, Senthivel VR, Dekker L, 
            Caro-Astorga J, Buat MLA, Song W, Lee KY, Ellis T
  TITLE     Komagataeibacter Tool Kit (KTK): A Modular Cloning System for 
            Multigene Constructs and Programmed Protein Secretion from Cellulose
            Producing Bacteria.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3422-3434. doi: 
            10.1021/acssynbio.1c00358. Epub 2021 Nov 12.
  PUBMED    34767345
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00358"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3422-3434"
COMMENT     Sequence Label: KTK_040
FEATURES             Location/Qualifiers
     source          1..1936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     137..156
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(375..479)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      582..1170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1071..1090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1450..1936,1..305,306..374))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
       61 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
      121 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      181 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      241 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      301 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
      361 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
      421 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
      481 acatttcccc gaaaagtgcc acctgtcatg accaaaatcc cttaacgtga gttttcgttc
      541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      721 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      961 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1201 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1261 gataaccgta gtcggtctca ttcttaactc ggtaccaaat tccagaaaag acacccgaaa
     1321 gggtgttttt tcgttttggt ccaacgtgag accaagatta tcaaaaagga tcttcaccta
     1381 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1441 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1501 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1561 atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc
     1621 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1681 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1741 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1801 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1861 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1921 gttatcactc atggtt
//
