LOCUS       sequence-359528-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of N-terminal half of Firefly luciferase 
            for split-Luciferase experiments (amino acids 2-416)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  TITLE     Najafov lab plasmids
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-N-Luc
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(542..559)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    548..772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cccgcgctcg ctacttagct tggtaccgag ctcggatcct gtccggttat gtaaacaatc
       61 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
      121 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
      181 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
      241 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
      301 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
      361 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
      421 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
      481 gcggaaagat cgccgtggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
      541 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      601 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
      661 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
      721 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
      781 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
      841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      901 cccgctccct tcgctttctt cccctccctt ctcgccacgt cgccggcttt ccccgt
//
