LOCUS       sequence-359552-        2182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Beltran J, Steiner PJ, Bedewitz M, Wei S, Peterson FC, Li Z, Hughes 
            BE, Hartley Z, Robertson NR, Medina-Cucurella AV, Baumer ZT, Leonard
            AC, Park SY, Volkman BF, Nusinow DA, Zhong W, Wheeldon I, Cutler SR,
            Whitehead TA
  TITLE     Rapid biosensor development using plant hormone receptors as 
            reprogrammable scaffolds.
  JOURNAL   Nat Biotechnol. 2022 Jun 20. pii: 10.1038/s41587-022-01364-5. doi: 
            10.1038/s41587-022-01364-5.
  PUBMED    35726092
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jun 20. pii: 10.1038/s41587-022-01364-5. doi: 
            10.1038/s41587-022-01364-5."
COMMENT     Sequence Label: pJS647
FEATURES             Location/Qualifiers
     source          1..2182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(550..1365)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(550..1365)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1273..1292
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1357..1376
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1590..2178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2079..2098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
       61 ttcctgcgtt atcccctgat tctgtggata accgtggtct catctggtca atcgtacgac
      121 gattcgacaa accacaaaca tacaaacact tcatcaaatc ctgctccgtc gaacaaaact
      181 tcgagatgcg cgtcggatgc acgcgcgacg tgatcgtcat cagtggatta ccggcgaaca
      241 catcaacgga aagactcgat atactcgacg acgaacggag agttaccgga ttcagtatca
      301 tcggaggcga acataggctg acgaattaca aatccgttac gacggtgcat cggttcgaga
      361 aagagaatcg gatctggacg gtggttttgg aatcttacgt cgttgatatg ccggaaggta
      421 actcggagga tgatactcgt atgtttgctg atacggttgt gaagcttaat ttgcagaaac
      481 tcgcgacggt tgctgaagct atggctcgta tgagacctca gcgtgttaca accaattaac
      541 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg
      601 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag
      661 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc
      721 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg
      781 agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc
      841 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat
      901 tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac
      961 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
     1021 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
     1081 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
     1141 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
     1201 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
     1261 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
     1321 taatcgcggc ctggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
     1381 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
     1441 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
     1501 gttgaaggat cagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2161 gggcggagcc tatggaaaaa cg
//
