LOCUS       sequence-359555-       10342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for stable expression of GFP1-9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Koraichi F, Gence R, Bouchenot C, Grosjean S, Lajoie-Mazenc I, Favre
            G, Cabantous S
  TITLE     High-content tripartite split-GFP cell-based assays to screen for 
            modulators of small GTPase activation.
  JOURNAL   J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 
            10.1242/jcs.210419.
  PUBMED    29192060
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2018-01-8"; volume: "131(1). pii: jcs.210419. doi"; 
            pages: " 10.1242/jcs.210419"
COMMENT     Sequence Label: pTRIP CMV GFP1-9
FEATURES             Location/Qualifiers
     source          1..10342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1527..1546)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1760..1779)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1879..1901
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1939..1957)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2025..2129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2130..2198,2199..2990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2348..2367)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3161..3749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3650..3669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3903..3920
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3990..4010)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3995..4324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4175..4310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5458..5523
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5653..5673
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6098..6232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6135..6154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6189..6208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6400..7033
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7080..7205
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7698..7931
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8116..8160
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8453..8570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8679..8982
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8983..9186
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9137..9157
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9183..9207
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      9288..9297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             10161..10341
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
       61 gagtgagagg ccttgacatt ataatagatt tagcaggaat tgaactagga gtggagcaca
      121 caggcaaagc tgcagaagta cttggaagaa gccaccagag atactcacga ttctgcacat
      181 acctggctaa tcccagatcc taaggattac attaagttta ctaacattta tataatgatt
      241 tatagtttaa agtataaact tatctaattt actattctga cagatattaa ttaatcctca
      301 aatatcataa gagatgatta ctattatccc catttaacac aagaggaaac tgagagggaa
      361 agatgttgaa gtaattttcc cacaattaca gcatccgtta gttacgactc tatgatcttc
      421 tgacacaaat tccatttact cctcacccta tgactcagtc gaatatatca aagttatgga
      481 cattatgcta agtaacaaat taccctttta tatagtaaat actgagtaga ttgagagaag
      541 aaattgtttg caaacctgaa tagcttcaag aagaagagaa gtgaggataa gaataacagt
      601 tgtcatttaa caagttttaa caagtaactt ggttagaaag ggattcaaat gcataaagca
      661 agggataaat ttttctggca acaagactat acaatataac cttaaatatg acttcaaata
      721 attgttggaa cttgataaaa ctaattaaat attattgaag attatcaata ttataaatgt
      781 aatttacttt taaaaaggga acatagaaat gtgtatcatt agagtagaaa acaatcctta
      841 ttatcacaat ttgtcaaaac aagtttgtta ttaacacaag tagaatactg cattcaatta
      901 agttgactgc agattttgtg ttttgttaaa attagaaaga gataacaaca atttgaatta
      961 ttgaaagtaa catgtaaata gttctacata cgttcttttg acatcttgtt caatcattga
     1021 tcgaagttct ttatcttgga agaatttgtt ccaaagactc tgaaataagg aaaacaatct
     1081 attatatagt ctcacacctt tgttttactt ttagtgattt caatttaata atgtaaatgg
     1141 ttaaaattta ttcttctctg agatcatttc acattgcaga tagaaaacct gagactgggg
     1201 taatttttat taaaatctaa tttaatctca gaaacacatc tttattctaa catcaatttt
     1261 tccagtttga tattatcata taaagtcagc cttcctcatc tgcaggttcc acaacaaaaa
     1321 tccaaccaac tgtggatcaa aaatattggg aaaaaattaa aaatagcaat acaacaataa
     1381 aaaaatacaa atcagaaaaa cagcacagta taacaacttt atttagcatt tacaatctat
     1441 taggtattat aagtaatcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     1501 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     1561 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     1621 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     1681 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     1741 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     1801 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1861 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1921 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1981 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2041 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2101 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2161 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2221 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2281 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2341 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2401 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2461 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2521 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2581 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2641 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2701 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2761 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2821 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2881 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2941 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3001 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3061 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3121 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3181 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3241 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3301 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3361 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3421 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3481 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3541 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3601 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3661 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3721 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3781 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3841 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3901 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3961 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4021 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4081 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4141 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4201 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4261 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4321 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     4381 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     4441 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     4501 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     4561 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     4621 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     4681 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     4741 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     4801 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     4861 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     4921 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     4981 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     5041 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     5101 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     5161 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     5221 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     5281 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     5341 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     5401 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     5461 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     5521 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     5581 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     5641 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     5701 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     5761 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     5821 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     5881 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     5941 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     6001 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     6061 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     6121 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6181 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     6241 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     6301 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     6361 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     6421 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     6481 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     6541 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     6601 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     6661 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     6721 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     6781 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     6841 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6901 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6961 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7021 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     7081 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     7141 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     7201 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     7261 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     7321 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     7381 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     7441 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     7501 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     7561 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7621 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7681 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7741 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     7801 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7861 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     7921 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     7981 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     8041 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     8101 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     8161 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     8221 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8281 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     8341 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     8401 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa
     8461 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     8521 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     8581 acagggacag cagagatcca ctttggctga tacgcgtcta gttattaata gtaatcaatt
     8641 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8701 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8761 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8821 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     8881 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     8941 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     9001 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     9061 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     9121 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     9181 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
     9241 catagaagac accgggaccg atccagcctc cgcggtctag agctagcgcc accatggtgc
     9301 gcaagggcga ggagctgttc accggcgtgg tgcccatcct gatcgagctg gacggcgacg
     9361 tgaacggcca caagttcttc gtgcgcggcg agggcgaggg cgacgccacc atcggcaagc
     9421 tgagcctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctggtga
     9481 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcgccacg
     9541 acttcttcaa gagcgccatg cccgagggct acgtgcagga gcgcaccatc tacttcaagg
     9601 acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     9661 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggc cacaagctgg
     9721 agtacaactt caacagccac aaggtgtaca tcaccgccga caagcagaac aacggcatca
     9781 aggccaactt caccatccgc cacaacgtgg aggacggcag cgtgcagctg gccgaccact
     9841 accagcagaa cacccccatc ggcgacggcc ccgtgtgagg atccagcggc cgctcgagac
     9901 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     9961 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
    10021 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    10081 ctggaagggc taattcactc ccaacgaaga caaaatcgtc gagagatgct gcatataagc
    10141 agctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    10201 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    10261 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    10321 cagtgtggaa aatctctagc ag
//
