LOCUS       sequence-359566-        9174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses a truncated force-insensitive DSG-3 TSmod.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Jin X, Rosenbohm J, Kim E, Safa BT, Moghaddam AO, Seiffert-Sinha K, 
            Leiker M, Jones E, Zhai H, Baddam SR, Minnick G, Huo Y, Wahl JK 3rd,
            Meng F, Huang C, Lim JY, Conway DE, Sinha AA, Yang R
  TITLE     Desmosomal cadherin tension loss in pemphigus vulgaris mediated by 
            the inhibition of active RhoA at cell-cell adhesions.
  JOURNAL   iScience. 2025 Jul 9;28(8):113081. doi: 10.1016/j.isci.2025.113081. 
            eCollection 2025 Aug 15.
  PUBMED    40740498
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2025-07-9"; volume: "28(8):113081. doi"; pages: " 
            10.1016/j.isci.2025.113081. eCollection 2025 Aug 15"
COMMENT     Sequence Label: pcdna 3.1 DSG-3 Tailless Control
FEATURES             Location/Qualifiers
     source          1..9174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..55)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        227..606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        607..810
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     761..781
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     855..874
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        855..873
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3168..3875
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(3996..3998,3999..4001,4002..4712)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4041..4062)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4302..4321)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4649..4670
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4757..4774)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4763..4987
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5033..5461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5120..5139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5330..5351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5470..5490)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5475..5804
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5655..5790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5717..5736
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5871..6665
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5925..5944)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6535..6554
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6839..6960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6876..6895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6930..6949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7009..7025)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7009..7025)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7022..7044)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7033..7049
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7057..7063,7064..7081,7082..7087))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7102..7123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7240..7257)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7411..7999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7491..7510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8170..8961,8962..9030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8793..8812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9031..9135)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
       61 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
      121 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
      181 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
      241 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      301 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      361 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      421 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      481 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      541 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      601 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      661 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      721 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      781 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
      841 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac
      901 ttaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattca ctagtgattt
      961 attcaccaga cacaccagca acaatgacct gcctcttccc tagagccttg gggtctctgg
     1021 ccctcctgat ggtggtgctg ctggtacagg gggaactgca tgtgaagccg ggaggacagc
     1081 acagagaaga tgggacagca ttgcagctgg ccaaaagaag gtacaaacgt gaatgggtga
     1141 aatttgcaaa accctgcaga gaaagagaag acaactcaag acgaaaccca attgccaaga
     1201 tcacttcaga tttccaaaaa aaccagaaaa ttacctaccg catttctgga gtgggaattg
     1261 atcagcctcc ctttgggatc tttgttgttg acccaaacaa tggtgatatc aacataacag
     1321 ccatagttga tcgtgaggaa accccaagct tcctgatcac atgccgtgca ctaaatgccc
     1381 tgggacagga tgtagagaga ccacttatat taacagtgaa aattttagac gtcaatgata
     1441 accctccaat attttcacaa accatattca agggtgaaat tgaagaaaat agtgcttcaa
     1501 attccctggt aatgatccta aatgccacgg atgcagatga accaaaccat atgaactcta
     1561 aaattgcctt caaaattgtc tcccaggagc ccgcaggcat gtccatgttc ctcatcagca
     1621 gaaacaccgg agaagtccgc actttgacca gttctcttga tcgagagcaa attagtagct
     1681 accatctggt tgtgagcggt gcagacaacg atggaacagg actgtcaact cagtgtgaat
     1741 gtagtattaa aattaaagat gtcaatgaca acttcccagt gctcagagag tctcagtatt
     1801 cagcacgtat tgaagaaaat actttgaatg cagagctact ccgatttcaa gtgacagact
     1861 gggatgaaga atacacagat aattggttgg ctgtgtattt cttcacctct gggaatgagg
     1921 ggaattggtt tgaaattgaa acagatccta gaaccaatga aggcatcctg aaggtggtta
     1981 aggctctgga ttatgaacaa gtgcagagta tgcagtttag tattgctgtc agaaacaaag
     2041 ctgagttcca ccagtcagtg atctctcagt accgagtgca gtcaacgcca gtcacaattc
     2101 aagtcattga tgtccgagag ggaatctcat tccgtcctcc ttccaagaca tttactgtgc
     2161 aaaggggtgt aagcactaac aaattagttg gttacatcct ggggacatac caagctactg
     2221 atgaggacac gggtaaagct gcctcctctg tcagatatgt cctgggtcgt aatgatggtg
     2281 gtttgcttgt tattgattca aagactgctc agatcaaatt tgtcaaaaac atcgaccgag
     2341 attctacttt catagttaac aagacaattt ctgctgaggt tctggccata gacgaaaaca
     2401 ctggtaagac ctccacaggc acaatatatg tggaagtgcc cagttttaat gaaaactgcc
     2461 cgtcagttgt cctcgagaag aaagacatct gtacttcctc accttctgtg accctctcag
     2521 tgagaaccct ggacaggggt aaatacactg gcccctacac agtgtccctg gaggagcagc
     2581 cactgaagct gccagtcatg tggaccatca cgacgctgaa tgctacatca gctcttctac
     2641 aagcccagca gcaagtgtct ccaggagtgt acaatgtccc agtcatcgtg aaggacaatc
     2701 aggatgggct gtgtgacaca ccagagagct tgaccctgac agtgtgtcaa tgtgatgacc
     2761 ggagcatgtg cagagcccct atcccgagca gagagcctaa cacatatggg gagtcatcct
     2821 ggaggctggg gcctgctgcc attggcttga tcctgctagg actcctgatg ttgctattgg
     2881 ccccacttct gcttctgacc tgtgactgtg ggtcaggtcc cattggagga gcagcgacag
     2941 gtggctttat cccagtgccc gatggttcgg aaggtacaat tcatcagtgg ggaatcgaag
     3001 gagctcagcc cgaagacaag gaaatcacaa atatttgtgt tcctccagtt acaaccaatg
     3061 gagccgattt tatggaaagc tctgaagttt gcacaaatac atatgctggt gggaccatgg
     3121 tagaaggtgc ttcaggaatg gaaatgatta ccaagctggg agtcgagatg gtgagcaagg
     3181 gcgaggagac cacaatgggc gtaatcaagc ccgacatgaa gatcaagctg aagatggagg
     3241 gcaacgtgaa tggccacgcc ttcgtgatcg agggcgaggg cgagggcaag ccctacgacg
     3301 gcaccaacac catcaacctg gaggtgaagg agggagcccc cctgcccttc tcctacgaca
     3361 ttctgaccac cgcgttcgcc tacggcaaca gggccttcac caagtacccc gacgacatcc
     3421 ccaactactt caagcagtcc ttccccgagg gctactcttg ggagcgcacc atgaccttcg
     3481 aggacaaggg catcgtgaag gtgaagtccg acatctccat ggaggaggac tccttcatct
     3541 acgagataca cctcaagggc gagaacttcc cccccaacgg ccccgtgatg cagaagaaga
     3601 ccaccggctg ggacgcctcc accgagagga tgtacgtgcg cgacggcgtg ctgaagggcg
     3661 acgtcaagca caagctgctg ctggagggcg gcggccacca ccgcgttgac ttcaagacca
     3721 tctacagggc caagaaggcg gtgaagctgc ccgactatca ctttgtggac caccgcatcg
     3781 agatcctgaa ccacgacaag gactacaaca aggtgaccgt ttacgagagc gccgtggccc
     3841 gcaactccac cgacggcatg gacgagctgt acaaggggcc aggtggtgca gggccaggtg
     3901 gtgcagggcc aggtggtgca gggccaggtg gtgcagggcc cggtggtgca ggtccaggtg
     3961 gtgcaggtcc aggtggtgca ggtccaggtg gtgctatggt gagcaagggc gaggagctgt
     4021 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4081 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     4141 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc
     4201 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4261 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4321 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4381 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4441 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     4501 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4561 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaaactga
     4621 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4681 ggatcactct cggcatggac gagctgtaca aggcggccgc tcgagtctag agggcccgtt
     4741 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     4801 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     4861 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     4921 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     4981 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5041 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5101 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5161 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5221 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5281 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5341 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5401 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5461 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5521 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5581 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5641 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5701 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5761 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5821 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     5881 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5941 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6001 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     6061 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6121 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6181 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6241 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6301 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6361 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6421 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6481 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6541 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6601 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6661 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     6721 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     6781 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     6841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6961 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7021 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7081 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7141 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7201 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7261 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7321 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7861 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7921 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8041 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8101 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8161 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8221 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8281 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8341 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8401 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8461 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8521 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8581 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8641 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8701 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8761 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8821 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8881 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8941 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9001 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9061 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9121 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatc
//
