LOCUS       sequence-359581-       10789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Koraichi F, Gence R, Bouchenot C, Grosjean S, Lajoie-Mazenc I, Favre
            G, Cabantous S
  TITLE     High-content tripartite split-GFP cell-based assays to screen for 
            modulators of small GTPase activation.
  JOURNAL   J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 
            10.1242/jcs.210419.
  PUBMED    29192060
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2018-01-8"; volume: "131(1). pii: jcs.210419. doi"; 
            pages: " 10.1242/jcs.210419"
COMMENT     Sequence Label: pTRIP TRE-BI GFP10-RHOA/RBD-GFP11
FEATURES             Location/Qualifiers
     source          1..10789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..316
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    10..28
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    46..64
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    188..206
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             436..1014
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="AAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADI
                     EVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKH
                     FCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTK
                     DGVREVFEMATRAALQARRGKKKSGCLVL"
     LTR             1321..1501
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3029..3048)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3262..3281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3381..3403
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3441..3459)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3527..3631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3632..3700,3701..4492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3850..3869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4663..5251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5152..5171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5405..5422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5492..5512)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5497..5826
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5677..5812
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          6960..7025
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7155..7175
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7600..7734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7691..7710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7902..8535
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8582..8707
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9200..9433
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9618..9662
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9955..10072
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    10126..10207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
       61 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
      121 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      181 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      241 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
      301 gaaccgtcag atcgcctgga ccgcggcacc atgggcgacc tgcccgacga ccactacctg
      361 tccacccaga ccatcctgag caaggacctg aacatcgatg gtggcggtgg ctctggaggt
      421 ggtgggtcct ccggagctgc catccgcaag aaactggtga ttgttggtga tggagcctgt
      481 ggaaagacat gcttgctcat agtcttcagc aaggaccagt tcccagaggt gtatgtgccc
      541 acagtgtttg agaactatgt ggcagatatc gaggtggatg gaaagcaggt agagttggct
      601 ttgtgggaca cagctgggca ggaagattat gatcgcctga ggcccctctc ctacccagat
      661 accgatgtta tactgatgtg tttttccatc gacagccctg atagtttaga aaacatccca
      721 gaaaagtgga ccccagaagt caagcatttc tgtcccaacg tgcccatcat cctggttggg
      781 aataagaagg atcttcggaa tgatgagcac acaaggcggg agctagccaa gatgaagcag
      841 gagccggtga aacctgaaga aggcagagat atggcaaaca ggattggcgc ttttgggtac
      901 atggagtgtt cagcaaagac caaagatgga gtgagagagg tttttgaaat ggctacgaga
      961 gctgctctgc aagctagacg tgggaagaaa aaatctggtt gccttgtctt gtaatctaga
     1021 gctagcatcg atgggcccgg atccagcggc cgctcgagac ctagaaaaac atggagcaat
     1081 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     1141 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     1201 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     1261 ccaacgaaga caaaatcgtc gagagatgct gcatataagc agctgctttt tgcttgtact
     1321 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     1381 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     1441 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     1501 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     1561 gagagtgaga ggccttgaca ttataataga tttagcagga attgaactag gagtggagca
     1621 cacaggcaaa gctgcagaag tacttggaag aagccaccag agatactcac gattctgcac
     1681 atacctggct aatcccagat cctaaggatt acattaagtt tactaacatt tatataatga
     1741 tttatagttt aaagtataaa cttatctaat ttactattct gacagatatt aattaatcct
     1801 caaatatcat aagagatgat tactattatc cccatttaac acaagaggaa actgagaggg
     1861 aaagatgttg aagtaatttt cccacaatta cagcatccgt tagttacgac tctatgatct
     1921 tctgacacaa attccattta ctcctcaccc tatgactcag tcgaatatat caaagttatg
     1981 gacattatgc taagtaacaa attacccttt tatatagtaa atactgagta gattgagaga
     2041 agaaattgtt tgcaaacctg aatagcttca agaagaagag aagtgaggat aagaataaca
     2101 gttgtcattt aacaagtttt aacaagtaac ttggttagaa agggattcaa atgcataaag
     2161 caagggataa atttttctgg caacaagact atacaatata accttaaata tgacttcaaa
     2221 taattgttgg aacttgataa aactaattaa atattattga agattatcaa tattataaat
     2281 gtaatttact tttaaaaagg gaacatagaa atgtgtatca ttagagtaga aaacaatcct
     2341 tattatcaca atttgtcaaa acaagtttgt tattaacaca agtagaatac tgcattcaat
     2401 taagttgact gcagattttg tgttttgtta aaattagaaa gagataacaa caatttgaat
     2461 tattgaaagt aacatgtaaa tagttctaca tacgttcttt tgacatcttg ttcaatcatt
     2521 gatcgaagtt ctttatcttg gaagaatttg ttccaaagac tctgaaataa ggaaaacaat
     2581 ctattatata gtctcacacc tttgttttac ttttagtgat ttcaatttaa taatgtaaat
     2641 ggttaaaatt tattcttctc tgagatcatt tcacattgca gatagaaaac ctgagactgg
     2701 ggtaattttt attaaaatct aatttaatct cagaaacaca tctttattct aacatcaatt
     2761 tttccagttt gatattatca tataaagtca gccttcctca tctgcaggtt ccacaacaaa
     2821 aatccaacca actgtggatc aaaaatattg ggaaaaaatt aaaaatagca atacaacaat
     2881 aaaaaaatac aaatcagaaa aacagcacag tataacaact ttatttagca tttacaatct
     2941 attaggtatt ataagtaatc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     3001 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     3061 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     3121 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     3181 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     3241 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     3301 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3361 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3421 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3481 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3541 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3601 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3661 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3721 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3781 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3841 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3901 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3961 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4021 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4081 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4141 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4201 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4261 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4321 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4381 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4441 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4501 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4561 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4621 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4681 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4741 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4801 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4861 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4921 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4981 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5041 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5101 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5161 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5221 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5281 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5341 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5401 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5461 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     5521 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5581 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5641 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5701 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5761 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     5821 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     5881 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     5941 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     6001 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     6061 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     6121 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     6181 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     6241 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     6301 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     6361 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     6421 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     6481 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     6541 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     6601 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     6661 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     6721 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     6781 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     6841 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     6901 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     6961 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     7021 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     7081 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     7141 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     7201 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     7261 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     7321 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     7381 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     7441 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     7501 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     7561 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     7621 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7681 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     7741 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     7801 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     7861 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     7921 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     7981 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     8041 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     8101 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     8161 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     8221 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     8281 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     8341 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8401 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8461 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8521 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8581 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8641 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8701 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8761 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8821 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8881 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8941 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     9001 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     9061 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9121 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     9181 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     9241 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     9301 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     9361 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9421 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9481 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     9541 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9601 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     9661 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     9721 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     9781 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     9841 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     9901 tgaacggatc tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa
     9961 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
    10021 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
    10081 ttacagggac agcagagatc cactttggct gatacgcgta cgtcggcagt gaaaaaaatg
    10141 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
    10201 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
    10261 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
    10321 ttaggaggcg tcggtgatgc cggcggcggt cacgtactcc agcagcacca tgtggtcgcg
    10381 cttctctccg gaggacccac cacctccaga gccaccgcca ccatcgatgc ctgtcttctc
    10441 cagcacctgg gcctcctttc gccgctgcag ctcacccatg tagctgagaa tacggctgtt
    10501 gcacaccagc aggctcttgg tggcttccag agcctgctct cgctgggagc aggctgccag
    10561 cagcttgcag gccccatccc tcatccggat ctcatgatct agtttcctct gcagctctgt
    10621 gtcctccagg ctgagtgcca tctgccggat gtagagcata ttgaggtcct ccaggatcat
    10681 ggtggccata tgaccggtga attctccagg cgatctgacg gttcactaaa cgagctctgc
    10741 ttatataggc ctcccaccgt acacgccacc tcgacataac gcgttcact
//
