LOCUS       sequence-359594-        8957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the FLAG-tagged CeNL (Blue Nanolantern) in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Yao Z, Brennan CK, Scipioni L, Chen H, Ng KK, Tedeschi G, 
            Parag-Sharma K, Amelio AL, Gratton E, Digman MA, Prescher JA
  TITLE     Multiplexed bioluminescence microscopy via phasor analysis.
  JOURNAL   Nat Methods. 2022 Jul;19(7):893-898. doi: 
            10.1038/s41592-022-01529-9. Epub 2022 Jun 23.
  PUBMED    35739310
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01529-9"; journalName: "Nat Methods"; date: 
            "2022-07"; volume: "19"; issue: "7"; pages: "893-898"
COMMENT     Sequence Label: pLenti_CMV:Flag-CeNL_SV40:PuroR
FEATURES             Location/Qualifiers
     source          1..8957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2210..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2349..2652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2653..2856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2807..2827
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2853..2877
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2936..2959
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(2960..2962,2963..3475)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(3002..3023)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3263..3282)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3610..3631
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4199..4219)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4204..4533
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4384..4519
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4543..5142
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4543..4562)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5039..5059
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5209..5797
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5262..5282)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5680..5691)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5884..6117
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6189..6310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6226..6245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6280..6299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6350..6485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6505..6524)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6506..6524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6534..6551)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6534..6550)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6543..6565)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6692..7147
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6779..6798)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6989..7010
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7173..7277
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7278..7346,7347..8138)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7496..7515)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8309..8897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8798..8817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
     2341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2641 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2821 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaatggacta
     2941 caaggacgac gatgacaagg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3001 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3061 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3121 cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta
     3181 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3241 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3301 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3361 caacatcctg gggcacaagc tggagtacaa ctactttagc gacaacgtct atatcaccgc
     3421 cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg
     3481 cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3541 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     3601 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggttgcata cactcgaaga
     3661 tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc ttgaacaggg
     3721 aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc aaaggattgt
     3781 cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt atgaaggtct
     3841 gagcggcgac caaatgggcc agatcgaaaa aatttttaag gtggtgtacc ctgtggatga
     3901 tcatcacttt aaggtgatcc tgcactatgg cacactggta atcgacgggg ttacgccgaa
     3961 catgatcgac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat
     4021 cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc
     4081 cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc ggctgtgcga
     4141 acgcattctg gcgtaaggat ccggactagt taacgcgtcg accagtggtc gaccctgtgg
     4201 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4261 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4321 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4381 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4441 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4501 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatgaccga gtacaagccc
     4561 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     4621 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     4681 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4741 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     4801 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     4861 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     4921 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     4981 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5041 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5101 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     5161 cagcgcccga ccgaaaggag cgcacgaccc catgcatcgc cagtcgacaa tcaacctctg
     5221 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5281 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5341 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5401 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5461 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5521 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5581 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5641 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5701 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5761 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
     5821 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5881 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5941 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6001 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6061 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6121 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6181 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6241 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6301 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6361 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6421 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6481 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6541 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6601 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6661 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6721 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6781 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6841 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6901 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6961 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7021 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7081 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7141 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8941 ggccttttgc tcacatg
//
