LOCUS       sequence-359596-        8954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Yao Z, Brennan CK, Scipioni L, Chen H, Ng KK, Tedeschi G, 
            Parag-Sharma K, Amelio AL, Gratton E, Digman MA, Prescher JA
  TITLE     Multiplexed bioluminescence microscopy via phasor analysis.
  JOURNAL   Nat Methods. 2022 Jul;19(7):893-898. doi: 
            10.1038/s41592-022-01529-9. Epub 2022 Jun 23.
  PUBMED    35739310
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01529-9"; journalName: "Nat Methods"; date: 
            "2022-07"; volume: "19"; issue: "7"; pages: "893-898"
COMMENT     Sequence Label: pLenti_CMV:Flag-dKeNLuc_SV40:PuroR
FEATURES             Location/Qualifiers
     source          1..8954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2210..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2349..2652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2653..2856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2807..2827
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2853..2877
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2936..2959
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2960..3622
                     /codon_start=1
                     /product="dimeric Keima-Red fluorescent protein"
                     /label=dKeima-Red
                     /translation="VSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVT
                     KGGPLPFAWDILSPLFQYGSIPFTKYPEDIPDYVKQSFPEGYTWERTMNFEDGAVCTVS
                     NDSSIQGNCFIYNVKISGTNFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALKL
                     EGGGHYLCEFKSTYKAKKPVRMPGYHYIDRKLDVTSHNRDYTSVEQCEIAIARHFLLG"
     CDS             3638..4153
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(4196..4216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4201..4530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4381..4516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4540..5139
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4540..4559)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5036..5056
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5206..5794
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5259..5279)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5677..5688)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5881..6114
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6186..6307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6223..6242)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6277..6296
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6347..6482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6502..6521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6503..6521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6531..6548)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6531..6547)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6540..6562)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6689..7144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6776..6795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6986..7007
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7170..7274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7275..7343,7344..8135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7493..7512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8306..8894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8795..8814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
     2341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2641 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2821 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaatggacta
     2941 caaggacgac gatgacaagg tgagtgtgat cgctaaacaa atgacctaca aggtttatat
     3001 gtcaggcacg gtcaatggac actactttga ggtcgaaggc gatggaaaag gaaagcctta
     3061 cgagggagag cagacagtaa agctcactgt caccaagggt ggacctctgc catttgcttg
     3121 ggatatatta tcaccactgt ttcagtacgg aagcatacca ttcaccaagt accctgaaga
     3181 catccctgat tatgtaaagc agtcattccc tgagggatat acatgggaga ggaccatgaa
     3241 ctttgaagat ggtgcagtgt gtactgtcag caatgattcc agcatccaag gcaactgttt
     3301 catctacaat gtcaaaatct ctggtacgaa ctttcctccc aatggacctg ttatgcagaa
     3361 gaagacacag ggctgggaac ccagcactga gcgtctcttt gcacgagatg gaatgctgat
     3421 aggaaacgat tatatggctc tgaagttgga aggaggtggt cactatttgt gtgaatttaa
     3481 atctacttac aaggcaaaga agcctgtgag gatgccaggg tatcactata ttgaccgcaa
     3541 actggatgta accagtcaca acagggatta cacatctgtt gagcagtgtg aaatagccat
     3601 tgcacgccac tttttgctcg gtgatatctc cggaggtatg gtcttcacac tcgaagattt
     3661 cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg aacagggagg
     3721 tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa ggattgtcct
     3781 gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg aaggtctgag
     3841 cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg tggatgatca
     3901 tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta cgccgaacat
     3961 gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca aaaagatcac
     4021 tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga tcaaccccga
     4081 cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc tgtgcgaacg
     4141 cattctggcg taaggatccg gactagttaa cgcgtcgacc agtggtcgac cctgtggaat
     4201 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4261 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4321 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4381 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4441 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4501 ggcttttttg gaggcctagg cttttgcaaa aagcttacca tgaccgagta caagcccacg
     4561 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     4621 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     4681 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     4741 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     4801 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     4861 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     4921 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4981 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5041 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     5101 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     5161 cgcccgaccg aaaggagcgc acgaccccat gcatcgccag tcgacaatca acctctggat
     5221 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5281 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5341 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5401 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5461 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5521 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5581 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5641 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5701 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5761 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa
     5821 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5881 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5941 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6001 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6061 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6121 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6181 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6241 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6301 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     6361 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6421 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6481 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     6541 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     6601 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6661 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     6721 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6781 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6841 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6901 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6961 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7021 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7081 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     7141 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7201 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7261 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7321 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7381 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7441 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     7501 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     7561 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     7621 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     7681 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     7741 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     7801 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7861 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7921 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7981 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8041 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8101 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     8461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     8521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     8581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8941 cttttgctca catg
//
