LOCUS       sequence-35960-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA,
            Min C, Jaskelioff M, Xiao Y, Wu CJ, Cameron LA, Perry SR, Zeid R, 
            Feinberg T, Kim M, Vande Woude G, Granter SR, Bosenberg M, Chu GC, 
            DePinho RA, Rimm DL, Chin L
  TITLE     Proinvasion metastasis drivers in early-stage melanoma are 
            oncogenes.
  JOURNAL   Cancer Cell. 2011 Jul 12;20(1):92-103.
  PUBMED    21741599
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell."; date: "2011-07-12"; volume: "20(1)"; pages: "92-103"
COMMENT     Sequence Label: pLenti6/V5-DEST-HSF1
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..50)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    103..127
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(129..500)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 ccggtacgcg tagaatcgag accgaggaga gggttaggga taggcttacc ttcgaaccgc
       61 gggccctcta gactcgagcg gccgccactg tgctggatat caaccacttt gtacaagaaa
      121 gttgggtagg agacagtggg gtccttggct ttgggaggct ccgagcctgt cagcagggag
      181 atggtggggt cctcggcgaa gccgtcccct tcggagaagt aggagccctc tcccagctca
      241 aacagcaccg gcaggtcgtt gctcccggtg tccacggagc cggggtccag caggaacagc
      301 ggctgcgctg tgtagtgcac cagctgcttc cctgaatccg ggctgctgtt ctctgcctcg
      361 ggaggcctgg ggggctcctg gggagacagg agctcttgga tactggccag gctgctgtca
      421 aggtcaggca ggctcatgtc gggcacggtc accgaggggc tgaacaggtc cagcagggca
      481 ctggtgtcca cgctgaagcc gtggctgctc agcatggtct gcaggttatc caggttggag
      541 tccatagcat ccaagtggtc actgagctca ttcttgtcca ggcaggctac gctgaggcac
      601 ttttcagggg tggaggtgg
//
