LOCUS       sequence-359620-       10917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Koraichi F, Gence R, Bouchenot C, Grosjean S, Lajoie-Mazenc I, Favre
            G, Cabantous S
  TITLE     High-content tripartite split-GFP cell-based assays to screen for 
            modulators of small GTPase activation.
  JOURNAL   J Cell Sci. 2018 Jan 8;131(1). pii: jcs.210419. doi: 
            10.1242/jcs.210419.
  PUBMED    29192060
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2018-01-8"; volume: "131(1). pii: jcs.210419. doi"; 
            pages: " 10.1242/jcs.210419"
COMMENT     Sequence Label: pTRIP TRE-BI GFP10-RHOB/RBD-GFP11
FEATURES             Location/Qualifiers
     source          1..10917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    56..74
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    91..109
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    163..181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    198..216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    963..1044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1098..1215
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1508..1552)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1737..1970
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2463..2588
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2635..3268
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3436..3570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3460..3479)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3995..4015)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4145..4210
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(5344..5673)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5358..5493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5658..5678
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(5748..5765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5919..6507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5999..6018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6678..7469,7470..7538))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7301..7320
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7539..7643)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7711..7729
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7767..7789)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7889..7908
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8122..8141
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             9669..9849
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(join(10863..10917,1..260))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
ORIGIN
        1 cgcctacctc gacatacgtt ctctatcact gatagggagt aaactcgaca tacgttctct
       61 atcactgata gggataaact cgacatacgt tctctatcac tgatagggag taaactcgac
      121 atacgttctc tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg
      181 agtaaactcg acatcgttct ctatcactga tagggagtaa actcgacata cgttctctat
      241 cactgatagg gagtaaactc gagtgaacgc gttatgtcga ggtggcgtgt acggtgggag
      301 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagatt tcgagctcgg
      361 tacccgggga tcctctagtc agctgacgcg ttatgtcgag gtggcgtgta cggtgggagg
      421 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt caccggtcat
      481 atggccacca tgatcctgga ggacctcaat atgctctaca tccggcagat ggcactcagc
      541 ctggaggaca cagagctgca gaggaaacta gatcatgaga tccggatgag ggatggggcc
      601 tgcaagctgc tggcagcctg ctcccagcga gagcaggctc tggaagccac caagagcctg
      661 ctggtgtgca acagccgtat tctcagctac atgggtgagc tgcagcggcg aaaggaggcc
      721 caggtgctgg agaagacagg catcgatggt ggcggtggct ctggaggtgg tgggtcctcc
      781 ggagagaagc gcgaccacat ggtgctgctg gagtacgtga ccgccgccgg catcaccgac
      841 gcctcctaat ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
      901 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      961 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1021 caaataaagc atttttttca ctgccgacgt acgcgtatca gccaaagtgg atctctgctg
     1081 tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt
     1141 tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc
     1201 ccttttcttt taaaattgtg gatgaatact gccatttgtg aattcggcga taccgtcgag
     1261 atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc
     1321 cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat
     1381 atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca
     1441 agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc
     1501 aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct
     1561 ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt
     1621 cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag
     1681 cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga
     1741 gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc
     1801 cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct
     1861 gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg
     1921 cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct attcccactg
     1981 ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact cctaatggtt
     2041 caatttttac tactttatat ttatataatt cacttctcca attgtccctc atatctcctc
     2101 ctccaggtct gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct
     2161 tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa
     2221 tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat
     2281 ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta
     2341 actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt atattttttc
     2401 tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata
     2461 ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta
     2521 ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt
     2581 cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag
     2641 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     2701 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     2761 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     2821 aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc
     2881 aggccacgcc tccctggaaa gtccccagcg gaaagtccct tgtagcaagc tcgatatcag
     2941 cagttcttga agtactccgg atgcagctct cgggccacgt gatgaaatgc taggcggctg
     3001 tcaaacctcc actctaacac ttctctctcc gggtcatcca tcccatgcag gctcacaggg
     3061 tgtaacaagc tggtgttctc tcctttattg gcctcttcta ccttatctgg ctcaactggt
     3121 actagcttgt agcaccatcc aaaggtcagt ggatatctga cccctggccc tggtgtgtag
     3181 ttctgctaat cagggaagta gccttgtgtg tggtagatcc acagatcaag gatatcttgt
     3241 cttctttggg agtgaattag cccttccaac tactaagttt gtagtacata tttaacaaat
     3301 acaatttctt taaaatgaaa ataattcaga ggaatcacag gtttagagta aatgaaacca
     3361 caggtaattg gcagtggtaa tagggtatgg ggtgggaagt ttgggatgat tttggttagc
     3421 ttgagttatc cagttgatcc agacatgata agatacattg atgagtttgg acaaaccaca
     3481 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3541 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3601 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     3661 atggctgatt atgatctcta gtcaaggcac tatacatcaa atattcctta ttaacccctt
     3721 tacaaattaa aaagctaaag gtacacaatt tttgagcata gttattaata gcagacactc
     3781 tatgcctgtg tggagtaaga aaaaacagta tgttatgatt ataactgtta tgcctactta
     3841 taaaggttac agaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg
     3901 tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac
     3961 ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag
     4021 tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa
     4081 acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg
     4141 gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt
     4201 ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac
     4261 agaagtaagg ttccttcaca aagatccggg gcccactcat aaatccagtt gccgccacgg
     4321 tagccaatca ccgtatcgta taaatcatcg tcggtacgtt cggcatcgct catcacaata
     4381 cgtgcctgga cgtcgaggat ttcgcgtggg tcaatgccgc gccagatcca catcagacgg
     4441 ttaatcatgc gataccagtg agggatggtt ttaccatcaa gggccgactg cacaggcggt
     4501 tgtgcgccgt gattaaagcg gcggactagc gtcgaggttt caggatgttt aaagcggggt
     4561 ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg cagcctccag aatacttact
     4621 ggaaactatt gtaacccgcc tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg
     4681 aaaagattag cgaccggaga ttggcgggac gaatacgacg cccatatccc acggctgttc
     4741 aatccaggta tcttgcggga tatcaacaac atagtcatca accagcggac gaccagccgg
     4801 ttttgcgaag atggtgacaa agtgcgcttt tggatacatt tcacgaatcg caaccgcagt
     4861 accaccggta tccaccaggt catcaataac gatgaagcct tcgccatcgc cttctgcgcg
     4921 tttcagcact ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac aaacggtatc
     4981 gacatgacga atacccagtt cacgcgccag taacgcaccc ggtacagacc gccacggctt
     5041 acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag tttacgtgca
     5101 tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag tgtcccagcc
     5161 tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag attatagaga
     5221 tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc actgcctctc
     5281 cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct tgtgtccaag
     5341 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     5401 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     5461 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     5521 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5581 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5641 gcctggggac tttccacacc ctaactgaca cacattccac agctgcatta atgaatcggc
     5701 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5761 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5821 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5881 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5941 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6001 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6061 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6121 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6181 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6241 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6301 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6361 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6421 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6481 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6541 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6601 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6661 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6721 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6781 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6841 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6901 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6961 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7021 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7081 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7141 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7201 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7261 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7321 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7381 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7441 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7501 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7561 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7621 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     7681 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     7741 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     7801 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     7861 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     7921 cattcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     7981 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     8041 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     8101 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     8161 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctggctag attacttata
     8221 atacctaata gattgtaaat gctaaataaa gttgttatac tgtgctgttt ttctgatttg
     8281 tattttttta ttgttgtatt gctattttta attttttccc aatatttttg atccacagtt
     8341 ggttggattt ttgttgtgga acctgcagat gaggaaggct gactttatat gataatatca
     8401 aactggaaaa attgatgtta gaataaagat gtgtttctga gattaaatta gattttaata
     8461 aaaattaccc cagtctcagg ttttctatct gcaatgtgaa atgatctcag agaagaataa
     8521 attttaacca tttacattat taaattgaaa tcactaaaag taaaacaaag gtgtgagact
     8581 atataataga ttgttttcct tatttcagag tctttggaac aaattcttcc aagataaaga
     8641 acttcgatca atgattgaac aagatgtcaa aagaacgtat gtagaactat ttacatgtta
     8701 ctttcaataa ttcaaattgt tgttatctct ttctaatttt aacaaaacac aaaatctgca
     8761 gtcaacttaa ttgaatgcag tattctactt gtgttaataa caaacttgtt ttgacaaatt
     8821 gtgataataa ggattgtttt ctactctaat gatacacatt tctatgttcc ctttttaaaa
     8881 gtaaattaca tttataatat tgataatctt caataatatt taattagttt tatcaagttc
     8941 caacaattat ttgaagtcat atttaaggtt atattgtata gtcttgttgc cagaaaaatt
     9001 tatcccttgc tttatgcatt tgaatccctt tctaaccaag ttacttgtta aaacttgtta
     9061 aatgacaact gttattctta tcctcacttc tcttcttctt gaagctattc aggtttgcaa
     9121 acaatttctt ctctcaatct actcagtatt tactatataa aagggtaatt tgttacttag
     9181 cataatgtcc ataactttga tatattcgac tgagtcatag ggtgaggagt aaatggaatt
     9241 tgtgtcagaa gatcatagag tcgtaactaa cggatgctgt aattgtggga aaattacttc
     9301 aacatctttc cctctcagtt tcctcttgtg ttaaatgggg ataatagtaa tcatctctta
     9361 tgatatttga ggattaatta atatctgtca gaatagtaaa ttagataagt ttatacttta
     9421 aactataaat cattatataa atgttagtaa acttaatgta atccttagga tctgggatta
     9481 gccaggtatg tgcagaatcg tgagtatctc tggtggcttc ttccaagtac ttctgcagct
     9541 ttgcctgtgt gctccactcc tagttcaatt cctgctaaat ctattataat gtcaaggcct
     9601 ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga
     9661 actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     9721 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     9781 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     9841 gagagaccca gtacaagcaa aaagcagctg cttatatgca gcatctctcg acgattttgt
     9901 cttcgttggg agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga
     9961 tctacagctg ccttgtaagt cattggtctt aaaggtacct gaggtgtgac tggaaaaccc
    10021 acctcctcct cctcttgtgc ttctagccag gcacaatcag cattggtagc tgctgtattg
    10081 ctacttgtga ttgctccatg tttttctagg tctcgagcgg ccgctggatc cgggcccatc
    10141 gatgctagct ctagattata gcaccttgca gcagttgatg cagccgttct gggagccgta
    10201 gcgcttctgc agcgcggcgc gcgtggccgt ctcgaagacc tcgcgcacgc cttccttggt
    10261 cttggcagag cactcgaggt agtcgtaggc ttggatgcgc acggccatgg cgcggccgtc
    10321 atccgtgcgc acgggttcct gcttcatgcg ggccagctct gtgcggacat gctcgtcgct
    10381 gcgcaggtct tttttgttgg ccaccaggat gatgggcaca ttgggacaga agtgcttcac
    10441 ctcggggacc cacttctcgg ggatgttctc cagcgagtcc gggctgtcca ccgagaagca
    10501 catgagaatg acgtcggtgt ccgggtagga gagcggccgc aggcggtcgt agtcctcctg
    10561 gcccgccgtg tcccacagcg ccagctccac ctgcttgccg tccacctcaa tgtcggccac
    10621 atagttctcg aagacggtgg gcacgtacac ctcggggaac tcgtccttac tgaacacgat
    10681 cagcaggcac gtcttgccac acgcgccgtc gcccaccacc accagcttct tgcggatggc
    10741 cgctccggag gacccaccac ctccagagcc accgccacca tcgatgttca ggtccttgct
    10801 caggatggtc tgggtggaca ggtagtggtc gtcgggcagg tcgcccatgg tgccgcggtc
    10861 caggcgatct gacggttcac taaacgagct ctgcttatat aggcctccca ccgtaca
//
