LOCUS       sequence-359623-        4583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HRE RedFirefly luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase 
            Reporter Plasmids.
  JOURNAL   Methods Mol Biol. 2022;2524:409-432. doi: 
            10.1007/978-1-0716-2453-1_32.
  PUBMED    35821490
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2022"; volume: "2524:409-432. doi"; pages: " 
            10.1007/978-1-0716-2453-1_32"
COMMENT     Sequence Label: 4xHRE_RE:RedF
FEATURES             Location/Qualifiers
     source          1..4583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    21..69
                     /note="synthetic polyadenylation signal"
     misc_feature    83..174
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     123..142
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        329..360
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    2045..2269
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2393..2981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2473..2492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3096..3198
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3199..4014
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3199..4014
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3272..3291)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4128..4556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4215..4234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4425..4446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacgaattc gtctcaggag aataaaatat ctttattttc attacatctg tgtgttggtt
       61 ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca
      121 aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctcgctgt
      181 caggaggtga ctacgtgctg cctagagatc tgtgactacg tgctgcctag gcggccgcgt
      241 gactacgtgc tgcctagtct agagtgacta cgtgctgcct agtccctcga ggatatcaaa
      301 tctggcctcg gcggccaagc ttagacacta gagggtatat aatggaagct cgacttccag
      361 cttggcaatc cggtactgtt ggtaaagcca ccaatggaga acatggagaa cgacgagaac
      421 attgtggtgg gacccaagcc gttctacccg atcgaggagg gttccgctgg cacccagctg
      481 cgcaagtaca tggagcgcta cgctaagctg ggtgctatcg ccttcaccaa cgccgtgacc
      541 ggtgtggatt actcctacgc cgagtacctg gagaagtcct gctgcctcgg aaaggccctg
      601 cagaactacg gtctggtcgt ggatggccgc attgccctct gctcggagaa ctgcgaggag
      661 ttcttcattc ccgtgatcgc tggtctgttc atcggagtcg gcgttgcccc aaccaacgag
      721 atctacaccc tgcgcgagct ggtccacagc ctgggtatca gcaagccgac catcgttttc
      781 tcgagcaaga agggcctcga caaggttatc accgtgcaaa aaaccgtcac caccatcaag
      841 accatcgtta tcctggattc caaggttgac taccgcggtt accagtgcct cgacaccttc
      901 atcaagcgta acaccccacc cggcttccaa gcctcctcct tcaaaaccgt ggaggttgat
      961 cgcaaggagc aggtggccct gatcatgaac tcctccggct ccaccggtct gccaaagggc
     1021 gtgcagctga cccacgagaa caccgtcacc cgcttctcgc atgcccgtga tcccatctac
     1081 ggcaaccagg tgtccccagg caccgccgtc ctgaccgtcg tgcccctcca ccacggcttc
     1141 ggcatgttca ccactctcgg ctacttgatc tgcggcttcc gtgtggtgat gctgactaag
     1201 ttcgacgagg agactttcct caagaccctg caagactaca agtgcactta cgtgatcctg
     1261 gtgccgaccc tgttcgccat cctgaacaag agcgagctgt tgaacaaata cgacctgtcg
     1321 aacctggtgg agatcgcttc cggcggcgcc ccactgtcga aggaagttgg cgaggccgtt
     1381 gctcgccgct tcaacctgcc cggcgtccgt cagggttacg gcctgaccga gactacctcg
     1441 gctatcatca tcacccccga gggcgacgat aagcccggcg cttccggcaa ggttgttcca
     1501 ctcttcaaag ccaaggtgat cgacctggac accaagaagt ccctgggtcc caaccgtcgc
     1561 ggagaagtgt gcgtcaaggg ccccatgctg atgaagggct acgtcaacaa cccagaggcc
     1621 accaaggagt tgatcgacga ggagggctgg ctgcacactg gtgacatcgg ttactacgac
     1681 gaagagaagc acttcttcat cgttgaccgc ctgaagtcct tgatcaagta caagggctac
     1741 caggttcccc ccgccgagct cgagagcgtc ctgctgcagc acccctccat tttcgatgcc
     1801 ggagtggccg gtgtccccga tcccgtggct ggcgagctgc caggtgccgt cgtcgtgctg
     1861 gagtccggca agaacatgac cgagaaggaa gtgatggatt acgtggcctc gcaagtgtcc
     1921 aacgccaagc gcctgcgcgg cggcgtgcgc ttcgtggatg aggtgcccaa gggcctcacc
     1981 ggaaagatcg acggtcgcgc tatccgcgag atcctcaaga agcccgtggc caagatgtga
     2041 gcttctgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2101 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2161 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2221 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc gctgtcatga
     2281 gacgaattct gacacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2341 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2401 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2461 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2521 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2581 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2641 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2701 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2761 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2821 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2881 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2941 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3001 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3061 tatcaaaaag gatcttcacc tagatccttt tgtgatgatc ggcacgtaag aggttccaac
     3121 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     3181 ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctc aaggccgcga
     3241 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     3301 caatcaggtg cgacaatcta ccgattgtat gggaagcccg atgcgccaga gttgtttctg
     3361 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     3421 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     3481 tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct
     3541 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3601 cctgtttgta attgtccttt taacagcgat cgcgtatttc gactcgctca ggcgcaatca
     3661 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3721 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3781 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3841 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3901 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3961 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctgatttttt
     4021 ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa
     4081 gcggatgaat ggcagaaatt cgatgataag ctgtcaaaca tgatgttacg cgccctgtag
     4141 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4201 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4321 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccag cgccctgata
     4381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4441 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4501 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4561 caaaatatta acgcttacaa ttt
//
