LOCUS       sequence-359626-        3844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AP-1 Renilla luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase 
            Reporter Plasmids.
  JOURNAL   Methods Mol Biol. 2022;2524:409-432. doi: 
            10.1007/978-1-0716-2453-1_32.
  PUBMED    35821490
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2022"; volume: "2524:409-432. doi"; pages: " 
            10.1007/978-1-0716-2453-1_32"
COMMENT     Sequence Label: 6xAP-1_RE::Renilla
FEATURES             Location/Qualifiers
     source          1..3844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    25..73
                     /note="synthetic polyadenylation signal"
     misc_feature    87..178
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     127..146
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        301..332
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    1306..1530
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1654..2242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1734..1753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2357..2459
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2460..3275
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2460..3275
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2533..2552)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3389..3817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3476..3495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3686..3707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggtacc gtctcaaatg ggagaataaa atatctttat tttcattaca tctgtgtgtt
       61 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
      121 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctcg
      181 ctgcagacta tgagtcaaac agcgtgactc aagttgagtc agttgactca cttgagtcac
      241 tgactcaggc atgctccctc gaggatatca aatctggcct cggcggccaa gcttagacac
      301 tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc
      361 caccaatgac ttccaaggtt tatgaccccg agcaacgcaa gagaatgata actgggcctc
      421 agtggtgggc cagatgtaaa caaatgaatg tccttgatag tttcatcaat tactacgaca
      481 gtgaaaagca tgcagaaaac gccgtaattt ttttgcatgg caacgcagcc tcctcttatt
      541 tgtggcgaca cgtagtcccg catatagagc cggtagctag gtgtattatc cctgatctga
      601 tcggaatggg aaagagtggc aagtcaggga atggatctta tcgccttctt gaccactaca
      661 agtatctgac ggcttggttc gaactcttga atttgccgaa gaaaatcatc ttcgtgggtc
      721 acgattgggg agcctgtctc gcgttccatt attcctacga gcatcaggac aagataaagg
      781 caatcgttca cgccgagtct gtagtagatg taatcgagtc ctgggacgaa tggccagata
      841 ttgaggagga tatagcattg attaaaagcg aggaagggga aaagatggtg ctggagaaca
      901 acttctttgt ggaaacaatg cttccttcaa aaatcatgag aaaactggaa cccgaggaat
      961 ttgccgctta tctcgaaccg tttaaagaaa agggagaagt ccgcaggcca acactttcct
     1021 ggccaagaga aatcccgctc gtcaaaggcg gaaagccgga tgttgtgcaa atagtccgaa
     1081 attacaacgc gtacctccgg gcctctgacg atctccctaa aatgtttata gaatcagatc
     1141 caggattttt tagcaacgct atcgtagaag gagcaaagaa atttccaaat accgaatttg
     1201 tcaaagtaaa gggactgcat ttcagtcaag aggacgctcc agacgagatg ggcaaataca
     1261 taaagtcctt tgtagagagg gtcctgaaga acgaacagtg agcttctgtg ccttctagtt
     1321 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     1381 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     1441 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     1501 ggcatgctgg ggatgcggtg ggctctatgg cgctgctttg agacggtacc tgacacggtt
     1561 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1621 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1681 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1741 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1801 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1861 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1921 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1981 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2041 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2101 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2161 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2281 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2341 ctagatcctt ttgtgatgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     2401 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     2461 tgagccatat tcaacgggaa acgtcttgct caaggccgcg attaaattcc aacatggatg
     2521 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     2581 accgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2641 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     2701 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     2761 tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     2821 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     2881 ttaacagcga tcgcgtattt cgactcgctc aggcgcaatc acgaatgaat aacggtttgg
     2941 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     3001 aaatgcataa acttttgcca ttctcaccgg attcagtagt cactcatggt gatttctcac
     3061 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     3121 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     3181 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     3241 tgcagtttca tttgatgctc gatgagtttt tctgattttt tttaaggcag ttattggtgc
     3301 ccttaaacgc ctggttgcta cgcctgaata agtgataata agcggatgaa tggcagaaat
     3361 tcgatgataa gctgtcaaac atgatgttac gcgccctgta gcggcgcatt aagcgcggcg
     3421 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3481 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3541 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3601 gattagggtg atggttcacg tagtgggcca gcgccctgat agacggtttt tcgccctttg
     3661 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3721 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     3781 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     3841 attt
//
