LOCUS       sequence-359627-        3451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FOXO NLuc luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase 
            Reporter Plasmids.
  JOURNAL   Methods Mol Biol. 2022;2524:409-432. doi: 
            10.1007/978-1-0716-2453-1_32.
  PUBMED    35821490
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2022"; volume: "2524:409-432. doi"; pages: " 
            10.1007/978-1-0716-2453-1_32"
COMMENT     Sequence Label: 3xDBE_RE:NLuc
FEATURES             Location/Qualifiers
     source          1..3451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    26..74
                     /note="synthetic polyadenylation signal"
     misc_feature    88..179
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     128..147
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        327..358
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    912..1136
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1261..1849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1341..1360)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1964..2066
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2067..2882
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2067..2882
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2140..2159)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2996..3424
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3083..3102)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3293..3314
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacctcgag cgtctcagct tggagaataa aatatcttta ttttcattac atctgtgtgt
       61 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
      121 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctctc
      181 gctgaattcg atcaagtaaa caactatgta aacaagatgc agatcaagta aacaactatg
      241 taaacaactc cgttgatcaa gtaaacaact atgtaaacaa tccctcgagg atatcaaatc
      301 tggcctcggc ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct
      361 tggcaatccg gtactgttgg taaagccacc aatggtgttt accttggagg acttcgtggg
      421 tgactggagg caaactgcag gatacaattt ggaccaggtc ctcgaacagg gaggagtcag
      481 cagccttttc caaaacttgg gcgtgtccgt tacccctatt cagagaattg tcctttccgg
      541 agagaacgga ttgaaaatag atatccacgt cataatcccg tatgaaggac tgtctgggga
      601 ccaaatgggt cagattgaaa aaatattcaa agtagtttat cccgttgatg atcatcactt
      661 caaagttatc cttcattatg ggactttggt aatcgatgga gttactccta atatgataga
      721 ttactttggc cgaccttacg aggggatcgc tgtattcgac ggcaagaaaa tcaccgtcac
      781 tggaaccctc tggaacggca acaagattat tgacgagcga ttgatcaatc cagacggatc
      841 tttgcttttc cgcgtaacga taaacggcgt aacagggtgg aggctctgcg aacgaatact
      901 cgcctgagct tctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
      961 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     1021 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     1081 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcgct
     1141 tggctgagac gctcgagtga cacggttatc cacagaatca ggggataacg caggaaagaa
     1201 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1261 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1321 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1381 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1441 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1501 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1561 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1621 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1681 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1741 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1801 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1861 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1921 catgagatta tcaaaaagga tcttcaccta gatccttttg tgatgatcgg cacgtaagag
     1981 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     2041 gattttcagg agctaaggaa gctaaaatga gccatattca acgggaaacg tcttgctcaa
     2101 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     2161 atgtcgggca atcaggtgcg acaatctacc gattgtatgg gaagcccgat gcgccagagt
     2221 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     2281 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2341 atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag gtattagaag
     2401 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     2461 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcga ctcgctcagg
     2521 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2581 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     2641 cagtagtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2701 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2761 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2821 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2881 gatttttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc ctgaataagt
     2941 gataataagc ggatgaatgg cagaaattcg atgataagct gtcaaacatg atgttacgcg
     3001 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3061 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3121 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3181 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccagcg
     3241 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3301 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3361 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3421 aattttaaca aaatattaac gcttacaatt t
//
