LOCUS       sequence-359633-       11149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing a UAS-driven destabilized GFP, as well 
            as a PGK promoter-driven iRFP marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ravindran PT, McFann S, Thornton RH, Toettcher JE
  TITLE     A synthetic gene circuit for imaging-free detection of signaling 
            pulses.
  JOURNAL   Cell Syst. 2022 Feb 16;13(2):131-142.e13. doi: 
            10.1016/j.cels.2021.10.002. Epub 2021 Nov 4.
  PUBMED    34739875
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.10.002"; journalName: "Cell Syst"; date: 
            "2022-02-16- 16"; volume: "13"; issue: "2"; pages: "131-142.e13"
COMMENT     Sequence Label: pHR pUAS-dGFP pPGK-iRFP
FEATURES             Location/Qualifiers
     source          1..11149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          455..520
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             650..670
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1095..1229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1132..1151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1186..1205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1397..2030
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2077..2202
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2699..2932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3117..3161
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3446..3563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3789..3813
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3894..3896,3897..3899,3900..4610,4611..4616,
                     4617..4739)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(3939..3960)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4200..4219)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4547..4568
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4862..5361
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4897..4920)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5294..5313
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5382..6332
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     misc_feature    6409..6997
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6462..6482)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6880..6891)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7000..7016)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7001..7017)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7107..7340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7365..7383)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7366..7383)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7673..7692)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7906..7925)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8025..8047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8085..8103)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8171..8275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8276..8344,8345..9136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8494..8513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9307..9895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9796..9815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10049..10066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10136..10156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10141..10470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10321..10456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
       61 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
      121 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
      181 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
      241 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
      301 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
      361 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      421 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      481 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      541 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
      601 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
      661 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
      721 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
      781 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
      841 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
      901 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
      961 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     1021 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1081 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1201 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     1261 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     1321 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     1381 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     1441 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     1501 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     1561 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     1621 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     1681 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     1741 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     1801 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     1861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     2041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     2101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     2161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     2221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     2281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     2341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     2401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     2461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     2521 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     2581 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     2641 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     2701 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     2761 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     2821 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     2881 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2941 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     3001 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     3061 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     3121 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     3181 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     3241 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     3301 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     3361 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     3421 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3481 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3541 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     3601 tcgataagct tgatatcgaa ttcggagcac tgtcctccga acgtcggagc actgtcctcc
     3661 gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc ctccgaacgg agcatgtcct
     3721 ccgaacgtcg gagcactgtc ctccgaacga ctagttaggc gtgtacggtg ggaggcctat
     3781 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     3841 gacctccata gaagacaccg ggaccgatcc agcctctcga cattcgttgg atcatggtga
     3901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3961 taaacggcta caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4081 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4321 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4381 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4561 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag aagcttagcc
     4621 atggcttccc gccggaggtg gaggagcagg atgatggcac gctgcccatg tcttgtgccc
     4681 aggagagcgg gatggaccgt caccctgcag cctgtgcttc tgctaggatc aatgtgtagg
     4741 atccttgact tgcggccgca actcccacct gcaacatgcg tgactgactg aggccgcgac
     4801 tctagagtcg acctgcatct aggcgccgga attagatctc tcgaggttaa cgaattctac
     4861 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     4921 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     4981 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     5041 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     5101 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     5161 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
     5221 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
     5281 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
     5341 tcctcatctc cgggcctttc gacctgcagc ccaagcttac catggctgaa ggatccgtcg
     5401 ccaggcagcc tgacctcttg acctgcgacg atgagccgat ccatatcccc ggtgccatcc
     5461 aaccgcatgg actgctgctc gccctcgccg ccgacatgac gatcgttgcc ggcagcgaca
     5521 accttcccga actcaccgga ctggcgatcg gcgccctgat cggccgctct gcggccgatg
     5581 tcttcgactc ggagacgcac aaccgtctga cgatcgcctt ggccgagccc ggggcggccg
     5641 tcggagcacc gatcactgtc ggcttcacga tgcgaaagga cgcaggcttc atcggctcct
     5701 ggcatcgcca tgatcagctc atcttcctcg agctcgagcc tccccagcgg gacgtcgccg
     5761 agccgcaggc gttcttccgc cgcaccaaca gcgccatccg ccgcctgcag gccgccgaaa
     5821 ccttggaaag cgcctgcgcc gccgcggcgc aagaggtgcg gaagattacc ggcttcgatc
     5881 gggtgatgat ctatcgcttc gcctccgact tcagcggcga agtgatcgca gaggatcggt
     5941 gcgccgaggt cgagtcaaaa ctaggcctgc actatcctgc ctcaaccgtg ccggcgcagg
     6001 cccgtcggct ctataccatc aacccggtac ggatcattcc cgatatcaat tatcggccgg
     6061 tgccggtcac cccagacctc aatccggtca ccgggcggcc gattgatctt agcttcgcca
     6121 tcctgcgcag cgtctcgccc gtccatctgg aattcatgcg caacataggc atgcacggca
     6181 cgatgtcgat ctcgattttg cgcggcgagc gactgtgggg attgatcgtt tgccatcacc
     6241 gaacgccgta ctacgtcgat ctcgatggcc gccaagcctg cgagctagtc gcccaggttc
     6301 tggcctggca gatcggcgtg atggaagagt aaagcggccg cgactctaga gtcgacctgc
     6361 aggcatgcaa gcttgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
     6421 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6481 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6541 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6601 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6661 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6721 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     6781 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     6841 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     6901 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     6961 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg
     7021 aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7081 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     7141 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7201 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7261 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7321 agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta
     7381 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat
     7441 atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg
     7501 taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc
     7561 ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac
     7621 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     7681 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     7741 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     7801 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     7861 atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga
     7921 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     7981 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     8041 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     8101 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     8161 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8221 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8281 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8341 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8401 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8461 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8521 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8581 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8641 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8701 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8761 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8821 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8881 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8941 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9001 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9061 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9121 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9181 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9241 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9361 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9421 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9481 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9541 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9601 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9661 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9721 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9781 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9841 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9901 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9961 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    10021 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    10081 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat
    10141 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10201 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
    10261 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
    10321 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
    10381 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
    10441 ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat
    10501 atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga
    10561 ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt
    10621 cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa
    10681 atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat
    10741 gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg
    10801 ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat
    10861 cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc
    10921 atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat
    10981 ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac
    11041 tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta
    11101 ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgc
//
