LOCUS       sequence-35968-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Scott KL, Nogueira C, Heffernan TP, van Doorn R, Dhakal S, Hanna JA,
            Min C, Jaskelioff M, Xiao Y, Wu CJ, Cameron LA, Perry SR, Zeid R, 
            Feinberg T, Kim M, Vande Woude G, Granter SR, Bosenberg M, Chu GC, 
            DePinho RA, Rimm DL, Chin L
  TITLE     Proinvasion metastasis drivers in early-stage melanoma are 
            oncogenes.
  JOURNAL   Cancer Cell. 2011 Jul 12;20(1):92-103.
  PUBMED    21741599
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell."; date: "2011-07-12"; volume: "20(1)"; pages: "92-103"
COMMENT     Sequence Label: pLenti6/V5-DEST-SPAG5
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..59)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cattactaac cggtacgcgt agaatcgaga ccgaggagag ggttagggat aggcttacct
       61 tcgaaccgcg ggccctctag actcgagcgg ccgccactgt gctggatatc aaccactttg
      121 tacaagaaag ttgggtagct cagaaattca gcaatccctg tagttctttg catcccctca
      181 ccacctctgg aatagagagc agggtcttat aaatatgctg aacaatgtca tctagttttt
      241 ctaactcctt gtcagagcgc cgaaggttct cctctaggat atttctatgg ctctggaact
      301 tgatcatgag tttttccttc tcatttttca tctccaggaa catcactctc agtttgtcca
      361 cctcctgaga gagccacact ttctcctgga tccaattggt ggccataggc tggcagttgg
      421 agtccagctg gcctgccagg gcctcctgga gcactttggt ctctgtctcc gcacgccgaa
      481 gtgagcgggt aaggtgggtc acctcctctc taaggcttat gagctcgcca ctcttgtcta
      541 tctgttctag gatcttctca ttctgctgct gtatcacttc ctggagctcc ttttcattct
      601 tgtagcgcaa gcacaaggcc tggttcagct tctctatgtc tgcttctttt gccttctgga
      661 cttcctgatg ctgttcttcc tgggcctgca gcct
//
