LOCUS       sequence-359681-       10006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The 3rd generation of rabies vector expressing Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lea NE, Lavin TK, Fu X, Matsuyama M, Hou 
            Y, Feng G, Wickersham IR
  TITLE     Third-generation rabies viral vectors allow nontoxic retrograde 
            targeting of projection neurons with greatly increased efficiency.
  JOURNAL   Cell Rep Methods. 2023 Nov 20;3(11):100644. doi: 
            10.1016/j.crmeth.2023.100644.
  PUBMED    37989085
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2023-11-20"; volume: "3(11):100644. doi"; pages: "
            10.1016/j.crmeth.2023.100644"
COMMENT     Sequence Label: pRV∆L-5Cre
FEATURES             Location/Qualifiers
     source          1..10006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(505..1296,1297..1365))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1128..1147
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1366..1470)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1497..1953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1584..1603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1794..1815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2079..2101
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2094..2110
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        2152..2531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2532..2735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2686..2706
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2780..2799
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2780..2798
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7833..7842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7845..7865
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9174..9192)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9188..9235
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(9258..9274)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9258..9274)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9311..9331)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9311..9329)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9350..9366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9350..9366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9363..9385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9374..9390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9398..9404,9405..9422,9423..9428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9443..9464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9581..9598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9752..10006,1..334))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9832..9851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
       61 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      181 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacccttgga
      661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     1261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     1321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc
     1501 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     1561 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     1621 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     1681 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     1741 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     1801 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     1861 gattttgccg atttcggcct attggttaaa aaaatgagct gatttaacaa aaatttaacg
     1921 cgaattttaa caaaatatta acgcttacaa tttccattcg ccattcaggc tgcgcaactg
     1981 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     2041 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     2101 gacggccagt gagcgcgcga taagcttgat atcgaattcc tgcagcgcgt tgacattgat
     2161 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     2221 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2281 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2341 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2401 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2461 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2521 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2581 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2641 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     2701 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
     2761 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagatta
     2821 agcgtctgat gagtccgtga ggacgaaacc cggcgtaccg ggtcacgctt aacaaccaga
     2881 tcaaagaaaa aacagacatt gtcaattgca aagcaaaaat gtaacacccc tacaatggat
     2941 gccgacaaga ttgtattcaa agtcaataat caggtggtct ctttgaagcc tgagattatc
     3001 gtggatcaat atgagtacaa gtaccctgcc atcaaagatt tgaaaaagcc ctgtataacc
     3061 ctaggaaagg ctcccgattt aaataaagca tacaagtcag ttttgtcagg catgagcgcc
     3121 gccaaactta atcctgacga tgtatgttcc tatttggcag cggcaatgca gttttttgag
     3181 gggacatgtc cggaagactg gaccagctat ggaattgtga ttgcacgaaa aggagataag
     3241 atcaccccag gttctctggt ggagataaaa cgtactgatg tagaagggaa ttgggctctg
     3301 acaggaggca tggaactgac aagagacccc actgtccctg agcatgcgtc cttagtcggt
     3361 cttctcttga gtctgtatag gttgagcaaa atatccgggc aaaacactgg taactataag
     3421 acaaacattg cagacaggat agagcagatt tttgagacag ccccttttgt taaaatcgtg
     3481 gaacaccata ctctaatgac aactcacaaa atgtgtgcta attggagtac tataccaaac
     3541 ttcagatttt tggccggaac ctatgacatg tttttctccc ggattgagca tctatattca
     3601 gcaatcagag tgggcacagt tgtcactgct tatgaagact gttcaggact ggtatcattt
     3661 actgggttca taaaacaaat caatctcacc gctagagagg caatactata tttcttccac
     3721 aagaactttg aggaagagat aagaagaatg tttgagccag ggcaggagac agctgttcct
     3781 cactcttatt tcatccactt ccgttcacta ggcttgagtg ggaaatctcc ttattcatca
     3841 aatgctgttg gtcacgtgtt caatctcatt cactttgtag gatgctatat gggtcaagtc
     3901 agatccctaa atgcaacggt tattgctgca tgtgctcctc atgaaatgtc tgttctaggg
     3961 ggctatctgg gagaggaatt cttcgggaaa gggacatttg aaagaagatt cttcagagat
     4021 gagaaagaac ttcaagaata cgaggcggct gaactgacaa agactgacgt agcactggca
     4081 gatgatggaa ctgtcaactc tgacgacgag gactactttt caggtgaaac cagaagtccg
     4141 gaggctgttt atactcgaat catgatgaat ggaggtcgac taaagagatc tcacatacgg
     4201 agatatgtct cagtcagttc caatcatcaa gcccgtccaa actcattcgc cgagtttcta
     4261 aacaagacat attcgagtga ctcataagaa gttgaataac aaaatgccgg aaatctacgg
     4321 attgtgtata tccatcatga aaaaaactaa cacccctcct ttcgaaccat cccaaacatg
     4381 agcaagatct ttgtcaatcc tagtgctatt agagccggtc tggccgatct tgagatggct
     4441 gaagaaactg ttgatctgat caatagaaat atcgaagaca atcaggctca tctccaaggg
     4501 gaacccatag aggtggacaa tctccctgag gatatggggc gacttcacct ggatgatgga
     4561 aaatcgccca accatggtga gatagccaag gtgggagaag gcaagtatcg agaggacttt
     4621 cagatggatg aaggagagga tcctagcttc ctgttccagt catacctgga aaatgttgga
     4681 gtccaaatag tcagacaaat gaggtcagga gagagatttc tcaagatatg gtcacagacc
     4741 gtagaagaga ttatatccta tgtcgcggtc aactttccca accctccagg aaagtcttca
     4801 gaggataaat caacccagac tactggccga gagctcaaga aggagacaac acccactcct
     4861 tctcagagag aaagccaatc atcgaaagcc aggatggcgg ctcaaattgc ttctggccct
     4921 ccagcccttg aatggtcggc taccaatgaa gaggatgatc tatcagtgga ggctgagatc
     4981 gctcaccaga ttgcagaaag tttctccaaa aaatataagt ttccctctcg atcctcaggg
     5041 atactcttgt ataattttga gcaattgaaa atgaaccttg atgatatagt taaagaggca
     5101 aaaaatgtac caggtgtgac ccgtttagcc catgacgggt ccaaactccc cctaagatgt
     5161 gtactgggat gggtcgcttt ggccaactct aagaaattcc agttgttagt cgaatccgac
     5221 aagctgagta aaatcatgca agatgacttg aatcgctata catcttgcta accgaacctc
     5281 tcccctcagt ccctctagac aataaaatcc gagatgtccc aaagtcaaca tgaaaaaaac
     5341 aggcaacacc actgataaaa tgaacctcct acgtaagata gtgaaaaacc gcagggacga
     5401 ggacactcaa aaatcctctc ccgcgtcagc ccctctggat gacgatgact tgtggcttcc
     5461 accccctgaa tacgtcccgc tgaaagaact tacaggcaag aagaacatga ggaacttttg
     5521 tatcaacgga agggttaaag tgtgtagccc gaatggttac tcgttcagga tcctgcggca
     5581 cattctgaaa tcattcgacg agatatattc tgggaatcat aggatgatcg ggttagtcaa
     5641 agtggttatt ggactggctt tgtcaggatc tccagtccct gagggcctga actgggtata
     5701 caaattgagg agaaccttta tcttccagtg ggctgattcc aggggccctc ttgaagggga
     5761 ggagttggaa tactctcagg agatcacttg ggatgatgat actgagttcg tcggattgca
     5821 aataagagtg attgcaaaac agtgtcatat ccagggcaga gtctggtgta tcaacatgaa
     5881 cccgagagca tgtcaactat ggtctgacat gtctcttcag acacaaaggt ccgaagagga
     5941 caaagattcc tctctgcttc tagaataatc agattatatc ccgcaaattt atcacttgtt
     6001 tacctctgga ggagagaaca tatgggctca actccaaccc ttgggagcaa tataacaaaa
     6061 aacatgttat ggtgccatta aaccgctgca tttcatcaaa gtcaagttga ttacctttac
     6121 attttgatcc tcttggatgt gaaaaaaact attaacatcc ctcaaaagag gcgcgccaag
     6181 gaaagatggt tcctcaggct ctcctgtttg taccccttct ggtttttcca ttgtgttttg
     6241 ggaaattccc tatttacacg ataccagaca agcttggtcc ctggagtccg attgacatac
     6301 atcacctcag ctgcccaaac aatttggtag tggaggacga aggatgcacc aacctgtcag
     6361 ggttctccta catggaactt aaagttggat acatcttagc cataaaagtg aacgggttca
     6421 cttgcacagg cgttgtgacg gaggctgaaa cctacactaa cttcgttggt tatgtcacaa
     6481 ccacgttcaa aagaaagcat ttccgcccaa caccagatgc atgtagagcc gcgtacaact
     6541 ggaagatggc cggtgacccc agatatgaag agtctctaca caatccgtac cctgactacc
     6601 gctggcttcg aactgtaaaa accaccaagg agtctctcgt tatcatatct ccaagtgtgg
     6661 cagatttgga cccatatgac agatcccttc actcgagggt cttccctagc gggaagtgct
     6721 caggagtagc ggtgtcttct acctactgct ccactaacca cgattacacc atttggatgc
     6781 ccgagaatcc gagactaggg atgtcttgtg acatttttac caatagtaga gggaagagag
     6841 catccaaagg gagtgagact tgcggctttg tagatgaaag aggcctatat aagtctttaa
     6901 aaggagcatg caaactcaag ttatgtggag ttctaggact tagacttatg gatggaacat
     6961 gggtctcgat gcaaacatca aatgaaacca aatggtgccc tcccgataag ttggtgaacc
     7021 tgcacgactt tcgctcagac gaaattgagc accttgttgt agaggagttg gtcaggaaga
     7081 gagaggagtg tctggatgca ctagagtcca tcatgacaac caagtcagtg agtttcagac
     7141 gtctcagtca tttaagaaaa cttgtccctg ggtttggaaa agcatatacc atattcaaca
     7201 agaccttgat ggaagccgat gctcactaca agtcagtcag aacttggaat gagatcctcc
     7261 cttcaaaagg gtgtttaaga gttgggggga ggtgtcatcc tcatgtgaac ggggtgtttt
     7321 tcaatggtat aatattagga cctgacggca atgtcttaat cccagagatg caatcatccc
     7381 tcctccagca acatatggag ttgttggaat cctcggttat cccccttgtg caccccctgg
     7441 cagacccgtc taccgttttc aaggacggtg acgaggctga ggattttgtt gaagttcacc
     7501 ttcccgatgt gcacaatcag gtctcaggag ttgacttggg tctcccgaac tgggggaagt
     7561 atgtattact gagtgcaggg gccctgactg ccttgatgtt gataattttc ctgatgacat
     7621 gttgtagaag agtcaatcga tcagaaccta cgcaacacaa tctcagaggg acagggaggg
     7681 aggtgtcagt cactccccaa agcgggaaga tcatatcttc atgggaatca cacaagagtg
     7741 ggggtgagac cagactgtaa ggtaccggac tggccgtcct ttcaacgatc caagtccatg
     7801 aaaaaaacta acacccctcc gctagcaaag ccgccaccat ggtgcccaag aagaagagga
     7861 aggtgagcaa cctgctgacc gtgcaccaga acctgcccgc cctgcccgtg gacgccacca
     7921 gcgacgaggt gcgcaagaac ctgatggaca tgttccgcga ccgccaggcc ttcagcgagc
     7981 acacctggaa gatgctgctg agcgtgtgcc gcagctgggc cgcctggtgc aagctgaaca
     8041 accgcaagtg gttccccgcc gagcccgagg acgtgcgcga ctacctgctg tacctgcagg
     8101 cccgcggcct ggccgtgaag accatccagc agcacctggg ccagctgaac atgctgcacc
     8161 gccgcagcgg cctgccccgc cccagcgaca gcaacgccgt gagcctggtg atgcgccgca
     8221 tccgcaagga gaacgtggac gccggcgagc gcgccaagca ggccctggcc ttcgagcgca
     8281 ccgacttcga ccaggtgcgc agcctgatgg agaacagcga ccgctgccag gacatccgca
     8341 acctggcctt cctgggcatc gcctacaaca ccctgctgcg catcgccgag atcgcccgca
     8401 tccgcgtgaa ggacatcagc cgcaccgacg gcggccgcat gctgatccac atcggccgca
     8461 ccaagaccct ggtgagcacc gccggcgtgg agaaggccct gagcctgggc gtgaccaagc
     8521 tggtggagcg ctggatcagc gtgagcggcg tggccgacga ccccaacaac tacctgttct
     8581 gccgcgtgcg caagaacggc gtggccgccc ccagcgccac cagccagctg agcacccggg
     8641 ccctggaggg catcttcgag gccacccacc gcctgatcta cggcgccaag gacgacagcg
     8701 gccagcgcta cctggcctgg agcggccaca gcgcccgcgt gggcgccgcc cgcgacatgg
     8761 cccgcgccgg cgtgagcatc cccgagatca tgcaggccgg cggctggacc aacgtgaaca
     8821 tcgtgatgaa ctacatccgc aacctggaca gcgagaccgg cgccatggtg cgcctgctgg
     8881 aggacggcga ctgatgacgt acgaccggat actcctggaa gcctgcccat gctaagactc
     8941 ttgtgtgatg tatcttgaaa aaaacaagat cctaaatctg aacctttggt tgtttgattg
     9001 tttttctcat ttttgttgtt tatttgttaa gcgtgggtcg gcatggcatc tccacctcct
     9061 cgcggtccga cctgggcatc cgaaggagga cgcacgtcca ctcggatggc taagggaggg
     9121 cggggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     9181 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     9241 aggaggaact atatccggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     9301 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     9361 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9421 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9481 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9541 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9601 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9661 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9721 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9781 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9841 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9901 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9961 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgt
//
