LOCUS       sequence-359706-        8941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Soluble light-dependent effector protein (HP1alpha fused to PGL-1) 
            for optodroplet formation or recruitment to CIBN localizers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Erdel F, Rademacher A, Vlijm R, Tunnermann J, Frank L, Weinmann R, 
            Schweigert E, Yserentant K, Hummert J, Bauer C, Schumacher S, Al 
            Alwash A, Normand C, Herten DP, Engelhardt J, Rippe K
  TITLE     Mouse Heterochromatin Adopts Digital Compaction States without 
            Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):236-249.e7. doi: 
            10.1016/j.molcel.2020.02.005. Epub 2020 Feb 25.
  PUBMED    32101700
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.02.005"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "236-249.e7"
COMMENT     Sequence Label: pPHR-mCherry-PGL-1-mHP1alpha
FEATURES             Location/Qualifiers
     source          1..8941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        37..340
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        341..544
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     495..515
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             601..2094
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2104..2811
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2517..2536
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5705..5826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5742..5761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5796..5815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5833..6288)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5970..5991)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6182..6201
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6315..6419
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6421..6778
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6421..6612
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6444..6464)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6629..6764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6813..7607
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6813..7607
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6867..6886)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7477..7496
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7795..7814)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7839..7886
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8215..8803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8704..8723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
       61 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      121 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      181 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
      241 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
      301 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      361 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      421 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
      481 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      541 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgaggccacc
      601 atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat
      661 cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct
      721 gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt
      781 gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac
      841 aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt
      901 aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg
      961 gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata
     1021 tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat
     1081 atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg
     1141 gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa
     1201 ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta
     1261 ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt
     1321 gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac
     1381 gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga
     1441 gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata
     1501 tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct
     1561 tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg
     1621 gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg
     1681 attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat
     1741 ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc
     1801 tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc
     1861 gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg
     1921 ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt
     1981 gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta
     2041 ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc agcagcccgg
     2101 gtcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     2161 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     2221 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     2281 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     2341 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     2401 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     2461 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     2521 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     2581 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     2641 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     2701 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     2761 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gatggaggct
     2821 aacaagcgag aaattgtgga tttcggtggg ctgagatcct actttttccc aaatttggcc
     2881 cactacatca cgaagaatga cgaagaactg ttcaacaata catctcaggc caataagttg
     2941 gctgctttcg tgttgggagc ttccaaagac gctcctggag acgaagacat tttggagatg
     3001 atcttgccga acgacgctaa cgctgccgtt attgctgctg gcatggacgt ttgtcttctt
     3061 ctcggagata aatttcgtcc gaaatttgat gctgctgctg agaaattgtc aggattggga
     3121 catgctcatg atctggtttc tgtcatcgat gacgacaaga agctcggaat gctcgcccgt
     3181 aaggccaagc tgaagaagac tgaagacgct aagattcttc aagctcttct caaagtaatc
     3241 gcaatcgatg atgccgccga aaagtttgtt gagctcacgg aacttgtcag ccaactggat
     3301 ctggactttg atgtttatgt cctaactaaa atcctcggac ttatctctga ggagactagc
     3361 gacgaggtcg acattatccg tgataacgtc gtcaacgcat ttgatagctg caagccgttg
     3421 ttgaaacagt tgatgttgga tggtccgaaa tctgaacctg ccgatccatt cattagtctc
     3481 ctcatggatc cactcgaaga gtctgttggg aaagttgtga accatatcgc tcaacttttc
     3541 gaggaagcga gtaaaaatga aggagacgag tcacttgtct tgagatctca actcggatat
     3601 cagctctttt tccttatcgt tcgttcactt gcagatggaa agcgcgaggt ttccaagaaa
     3661 atcctctctg ggattcctac gagtgtcaga gcggaagtct ttccaggatt gcaaagaagt
     3721 gtctacaaga gtgcggtgtt cctcggcaat cacatcatcc aagttctttt aggatctaag
     3781 aagtcttttg aggattggga tgttgttgga gtcgcgaaag acttggagag cgcctggaaa
     3841 agacgagcca ttgcggaact tatcaagaag ttccaagtct caattctaga gcaatgcttc
     3901 gataagcccg ttccactgat tcctcagagt ccactaaaca atgatgcggt gatcgataat
     3961 gtgaataaag ctctacagtt tgcactgtgg cttacagagt tttacggatc cgagaatgag
     4021 actgaagcgc tgggcgagct tcgtttcttg gattccactt caaaaaatct tctcgtcgat
     4081 agcttcaaga aatttgttca aggaatcaac tcgaagactc atgtcactcg aattgttgaa
     4141 tcactggaga agtgttgttt gtccgataca ccaagtggca gaaagagcaa tgtgcaacca
     4201 tccacttccc aacagcagga ttccgcctac acgaaagagg aaatgacgac ggttcacaac
     4261 acatacagcg taaatactaa agctcaagtt ctcaacggtc tcagtgatac caattcttcc
     4321 gggttacttg tcgattcaaa ggattcactc agtcttcagg agatttcttg tgacgaagtg
     4381 gattcttcga ctttgctaag ctcatcaaga aacattggag aaggtgttac tgtaaaagcg
     4441 gtcgatcctg tcccagaaaa ggttaacgat gcacaacaac agcaaacagt taacgagatc
     4501 gagatggctt cggatgccaa tcaagatact tcttcaagtg cttcgccaga agtagcgcca
     4561 agtttttcca ccgatggctg ggattctcca acgaaatcag tagctctgcc accgggcatg
     4621 cagcagatag atgaagagga aaccacagtt gctgacaaag attctacacc acaaccacaa
     4681 gctcgtgctg aaactgcatg ggggtcaggc gacgctactc caatgcctct tcctgccccg
     4741 actaatcaat acaaagtcag tggatttggg gaagccaaag ttgcaaaagg attcggtcaa
     4801 tttgctccca ccagttcagc ttatggagga ggaggtggtc gcggaggata tggcggtgga
     4861 gaccgtggag gcagaggtgg atacggcgga gatcgtggag gtagaggtgg atacggcggg
     4921 ggagaccgcg gaggacgagg tggatacggc ggagatcgtg gacgtggtgg ttacgggggt
     4981 cgtggtggac gcggaggttt ctccggaatg ggaaagaaga ccaagaggac agccgacagc
     5041 tcttcttcag aggatgagga ggaatatgtg gtggaaaagg tgttggacag gcgcatggtt
     5101 aaggggcaag tggaatatct gttgaagtgg aaaggctttt ctgaggagca caatacttgg
     5161 gaacctgaga agaacttgga ttgtcctgaa ctaatttctg agtttatgaa aaagtataag
     5221 aagatgaagg agggtgaaaa caataagccc agggagaaat cagaaggaaa caagaggaaa
     5281 tccagtttct ccaacagcgc tgatgatatt aaatctaaaa aaaagagaga gcaaagcaat
     5341 gatatcgctc ggggctttga gagaggactg gaaccagaaa agatcatcgg agcaacagat
     5401 tcctgcggtg acttaatgtt cttaatgaaa tggaaagaca cagatgaagc tgacctggtt
     5461 cttgcaaaag aagctaacgt gaagtgtcca cagattgtga tagcatttta tgaagagaga
     5521 ctgacgtggc acgcatatcc agaggatgcg gaaaacaaag aaaaagaaag cgcgaagagc
     5581 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5641 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5701 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5821 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     5881 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5941 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     6001 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     6061 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     6121 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     6181 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     6241 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     6301 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6361 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     6421 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     6481 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     6541 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6601 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     6661 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6721 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     6781 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6841 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6901 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6961 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     7021 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7081 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7141 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7201 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7261 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7321 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7381 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7441 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7501 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7561 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     7621 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     7681 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7741 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     7801 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     7861 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     7921 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     7981 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     8041 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     8101 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8161 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8221 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8281 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8341 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8401 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8461 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8521 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8581 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8641 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8701 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8761 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8821 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8881 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
     8941 c
//
