LOCUS       sequence-359712-        8432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of epidermal growth factor receptor (EGFR) 
            fused to mEGFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Tzoneva R, Stoyanova T, Petrich A, Popova D, Uzunova V, Momchilova 
            A, Chiantia S
  TITLE     Effect of Erufosine on Membrane Lipid Order in Breast Cancer Cell 
            Models.
  JOURNAL   Biomolecules. 2020 May 22;10(5). pii: biom10050802. doi: 
            10.3390/biom10050802.
  PUBMED    32455962
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomolecules."; date: "2020-05-22"; volume: "10(5). pii: 
            biom10050802. doi"; pages: " 10.3390/biom10050802"
COMMENT     Sequence Label: hEGFR-mEGFP
FEATURES             Location/Qualifiers
     source          1..8432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        84..441
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        84..275
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(107..127)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      292..427
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             476..1270
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             476..1270
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(530..549)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1140..1159
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1458..1477)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1502..1549
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1878..2466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2367..2386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2641..2944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2945..3148
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3099..3119
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(3298..3369,3370..5232,5233..5301,5302..6927)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     regulatory      6952..6961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6958..6960,6961..6963,6964..7677)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7003..7024)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7264..7283)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7611..7632
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7800..7921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7837..7856)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7891..7910
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7928..8383)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8065..8086)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8277..8296
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(8410..8432,1..82)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
       61 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
      121 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      181 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      241 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      301 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      361 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      421 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
      481 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      541 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      601 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
      661 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      721 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      781 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      841 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      901 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      961 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     1021 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     1081 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     1141 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     1201 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     1261 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     1321 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1381 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1441 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     1501 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     1561 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     1621 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     1681 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     1741 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1801 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1861 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1921 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1981 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2041 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2101 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2161 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2221 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2281 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2341 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2401 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2461 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2521 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     2581 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     2641 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2701 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2761 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2821 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     2881 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     2941 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3001 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3061 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3121 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagagc
     3181 ttatcgattc tagccgagtc cccgcctcgc cgccaacgcc acaaccaccg cgcacggccc
     3241 cctgactccg tccagtattg atcgggagag ccggagcgag ctcttcgggg agcagcgatg
     3301 cgaccctccg ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct ctgcccggcg
     3361 agtcgggctc tggaggaaaa gaaagtttgc caaggcacga gtaacaagct cacgcagttg
     3421 ggcacttttg aagatcattt tctcagcctc cagaggatgt tcaataactg tgaggtggtc
     3481 cttgggaatt tggaaattac ctatgtgcag aggaattatg atctttcctt cttaaagacc
     3541 atccaggagg tggctggtta tgtcctcatt gccctcaaca cagtggagcg aattcctttg
     3601 gaaaacctgc agatcatcag aggaaatatg tactacgaaa attcctatgc cttagcagtc
     3661 ttatctaact atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag aaatttacag
     3721 gaaatcctgc atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc
     3781 atccagtggc gggacatagt cagcagtgac tttctcagca acatgtcgat ggacttccag
     3841 aaccacctgg gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt
     3901 gcaggagagg agaactgcca gaaactgacc aaaatcatct gtgcccagca gtgctccggg
     3961 cgctgccgtg gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc aggctgcaca
     4021 ggcccccggg agagcgactg cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag
     4081 gacacctgcc ccccactcat gctctacaac cccaccacgt accagatgga tgtgaacccc
     4141 gagggcaaat acagctttgg tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg
     4201 acagatcacg gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat ggaggaagac
     4261 ggcgtccgca agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt
     4321 attggtgaat ttaaagactc actctccata aatgctacga atattaaaca cttcaaaaac
     4381 tgcacctcca tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc
     4441 acacatactc ctcctctgga tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc
     4501 acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag
     4561 aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct tgcagtcgtc
     4621 agcctgaaca taacatcctt gggattacgc tccctcaagg agataagtga tggagatgtg
     4681 ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt
     4741 gggacctccg gtcagaaaac caaaattata agcaacagag gtgaaaacag ctgcaaggcc
     4801 acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg
     4861 gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt
     4921 ctggagggtg agccaaggga gtttgtggag aactctgagt gcatacagtg ccacccagag
     4981 tgcctgcctc aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag
     5041 tgtgcccact acattgacgg cccccactgc gtcaagacct gcccggcagg agtcatggga
     5101 gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca cctgtgccat
     5161 ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac gaatgggcct
     5221 aagatcccgt ccatcgccac tgggatggtg ggggccctcc tcttgctgct ggtggtggcc
     5281 ctggggatcg gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg
     5341 ctgctgcagg agagggagct tgtggagcct cttacaccca gtggagaagc tcccaaccaa
     5401 gctctcttga ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct gggctccggt
     5461 gcgttcggca cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc
     5521 gtcgctatca aggaattaag agaagcaaca tctccgaaag ccaacaagga aatcctcgat
     5581 gaagcctacg tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc
     5641 ctcacctcca ccgtgcaact catcacgcag ctcatgccct tcggctgcct cctggactat
     5701 gtccgggaac acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc
     5761 gcaaagggca tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg
     5821 aacgtactgg tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaactg
     5881 ctgggtgcgg aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg
     5941 gcattggaat caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg
     6001 gtgaccgttt gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc
     6061 gagatctcct ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc
     6121 gatgtctaca tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc
     6181 cgtgagttga tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt
     6241 cagggggatg aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg
     6301 atggatgaag aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag
     6361 ggcttcttca gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc
     6421 agcaacaatt ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag
     6481 gaagacagct tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc
     6541 atagacgaca ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc
     6601 gctggctctg tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga
     6661 gacccacact accaggaccc ccacagcact gcagtgggca accccgagta tctcaacact
     6721 gtccagccca cctgtgtcaa cagcacattc gacagccctg cccactgggc ccagaaaggc
     6781 agccaccaaa ttagcctgga caaccctgac taccagcagg acttctttcc caaggaagcc
     6841 aagccaaatg gcatctttaa gggctccaca gctgaaaatg cagaatacct aagggtcgcg
     6901 ccacaaagca gtgaatttat tggagcaccg cgggcccggg atccaccggt cgccaccatg
     6961 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     7021 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     7081 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     7141 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     7201 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     7261 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     7321 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     7381 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     7441 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     7501 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     7561 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     7621 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     7681 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     7741 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     7801 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7861 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7921 aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     7981 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8041 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8101 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8161 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8221 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8281 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8341 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     8401 ggaaatgtgc gcggaacccc tatttgttta tt
//
