LOCUS       sequence-359736-        9201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Wnt - TCF/LEF gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE19
FEATURES             Location/Qualifiers
     source          1..9201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    293..881
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             399..410
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     808..828
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(973..1092)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(1096..2745)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      2742..2751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2778..2809)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    2921..3038
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3305..3349)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3534..3767
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4260..4385
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4432..4612
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4613..4839)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4867..4887)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4867..4885)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4906..4922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4906..4922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4919..4941)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4930..4946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4954..4960,4961..4978,4979..4984))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4999..5020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5137..5154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5308..5896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5388..5407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6067..6858,6859..6927))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6690..6709
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6928..7032)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7058..7513)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7195..7216)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7407..7426
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7640..7662
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7654..7671
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7655..7671
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7681..7700
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7681..7699
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7720..7855
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7895..8016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7906..7925)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7960..7979
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8088..8321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(8393..9106,9107..9109,9110..9112))
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8438..8459)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8787..8806
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9046..9067
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(join(9163..9201,1..278))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9177..9201,1..111)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
       61 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
      121 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
      181 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
      241 acttctgcct gctggggagc ctggggactt tccacaccgg taccatcgcc aggcggggag
      301 gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc
      361 agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag
      421 cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac
      481 gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag
      541 cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa
      601 gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca
      661 aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac
      721 agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga
      781 tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata
      841 ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgtcgatcat tactaaccgg
      901 tacgcgctga tgtctgtagt ctgaggtggg cgtgtgcaag ccgcagtgac gcgttctaga
      961 gtcgcggcct tagacgttga tcctggcgct ggcgcaagca gcagggtgtc tatccatgcc
     1021 gctctcctgg gcgcagctca tgggcagggt gccggcggcc tgctcctcca cctcgggagg
     1081 gaagccgtga gaattcacgg cgatcttgcc gcccttcttg gccttaatga gaatctcgcg
     1141 gatcttgcgg gcgtccaact tgccggtcag tcctttaggc acctcgtcca cgaacacaac
     1201 accaccgcgc agcttcttgg cggttgtaac ctggctggcc acatagtcca cgatctcctt
     1261 ctcggtcatg gttttaccgt gttccagcac gacgactgcg gcgggcagct cgccggcatc
     1321 gtcgtcgggc aggccggcga ccccggcgtc gaagatgttg gggtgttgca gcaggatgct
     1381 ctccagttcg gctggggcta cctggtagcc cttgtatttg atcaggctct tcagccggtc
     1441 cacgatgaag aagtgctcgt cctcgtccca gtaggcgatg tcgccgctgt gcagccagcc
     1501 gtccttgtcg atgagagcgt ttgtagcctc ggggttgtta acgtagccgc tcatgatcat
     1561 ggggccacgg acgcacagct cgccgcgctg gttcacaccc agtgtcttac cggtgtccaa
     1621 gtccaccacc ttagcctcga agaagggcac caccttgcct actgcgccag gcttgtcgtc
     1681 cccttcgggg gtgatcagaa tggcgctggt tgtttctgtc aggccgtagc cctggcggat
     1741 gcctggtagg tggaagcgtt tggccacggc ctcacctacc tccttgctga gcggcgcccc
     1801 gccgctggcg atctcgtgca agttgcttag gtcgtacttg tcgatgagag tgctcttagc
     1861 gaagaagcta aatagtgtgg gcaccagcag ggcagattga atcttatagt cttgcaagct
     1921 gcgcaagaat agctcctcct cgaagcggta catgagcacg acccgaaagc cgcagatcaa
     1981 gtagcccagc gtggtgaaca tgccgaagcc gtggtgaaat ggcaccacgc tgaggatagc
     2041 ggtgtcgggg atgatctggt tgccgaagat ggggtcgcgg gcatgactga atcggacaca
     2101 agcggtgcgg tgcggtaggg ctacgccctt gggcaatccg gtactgccac tactgttcat
     2161 gatcagggcg atggttttgt cccggtcgaa gctctcgggc acgaagtcgt actcgttgaa
     2221 gccgggtggc aaatgggaag tcacgaaggt gtacatgctt tggaagccct ggtagtcggt
     2281 cttgctatcc atgatgatga tcttttgtat gatcggtagc ttcttttgca cgttgaggat
     2341 cttttgcagc cctttcttgc tcacgaatac gacggtgggc tggctgatgc ccatgctgtt
     2401 cagcagctcg cgctcgttgt agatgtcgtt agctggggcc acagccacac cgatgaacag
     2461 ggcacccaac acgggcatga agaactgcaa gctattctcg ctgcacacca cgatccgatg
     2521 gtttgtattc agcccatagc gcttcatagc ttctgccagc cgaacgctca tctcgaagta
     2581 ctcggcgtag gtaatgtcca cctcgatatg tgcgtcggta aaggcgatgg tgccgggcac
     2641 cagggcgtag cgcttcatgg ctttgtgcag ctgctcgccg gcggtcccgt cttcgagtgg
     2701 gtagaatggc gctgggccct tcttaatgtt tttggcatct tccatggtgg ctttaccaac
     2761 agtaccggat tgccaagctg gaagtcgagc ttccattata taccctctag tgtctaagct
     2821 tggccgccga ggccagatct gttctcggat ctaccccctt tgatcttacc ccctttgatc
     2881 ttaccccctt tgatctgatc cacacgctgg tgcgatcgat aaaattttga atttttgtaa
     2941 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
     3001 ctgcactgta ccccccaatc cccccttttc ttttaaaagt taaccgatac cgtcgagatc
     3061 cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt
     3121 cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc
     3181 cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc
     3241 ctcctactat cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat
     3301 tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt
     3361 tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc
     3421 actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat
     3481 tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct
     3541 gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc
     3601 agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct
     3661 gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc
     3721 ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc
     3781 ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa
     3841 tttttactac tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc
     3901 caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc
     3961 tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag
     4021 agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt
     4081 tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact
     4141 gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt
     4201 ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg
     4261 acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc
     4321 accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga
     4381 gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga
     4441 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     4501 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     4561 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt
     4621 atcgagctag gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa
     4681 tccatgtcag acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca
     4741 ccaatcggca tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta
     4801 ccatgttgca agactacaag agtattgcat aagactacat taagcttgca gctccagctt
     4861 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     4921 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4981 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5041 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5101 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5281 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6061 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6121 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6181 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6241 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6301 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6361 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6421 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6481 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6541 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6601 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6661 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6721 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6781 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6841 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6901 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7021 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     7081 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     7141 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     7201 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     7261 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     7321 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     7381 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     7441 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     7501 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     7561 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     7621 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
     7681 taatacgact cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc
     7741 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     7801 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     7861 gagttagggg cgggatagct agagccagac atgataagat acattgatga gtttggacaa
     7921 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     7981 ttatttgtaa ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt
     8041 ctttgcaagt tataaatact gaataataag atgacatgaa ctactactgc tagagatttt
     8101 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     8161 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     8221 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa
     8281 aagcagatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa
     8341 aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta ccttacttgt
     8401 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
     8461 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
     8521 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
     8581 gcacgctgcc gtcctcgatg ttgtggcggg tcttgaagtt caccttgatg ccgttcttct
     8641 gcttgtcggc ggtgatatag accttgtggc tgttgtagtt gtactccagc ttgtgcccca
     8701 ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt
     8761 cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg
     8821 tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt
     8881 ggtcggggta gcgggcgaag cactgcacgc cgtaggtgaa ggtggtcacg agggtgggcc
     8941 agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg
     9001 catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct
     9061 cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atgctaggtg
     9121 ctgatcagat ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa agcctaggcc
     9181 tccaaaaaag cctcctcact a
//
