LOCUS       sequence-359746-       11296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Ng YH, Chanda S, Janas JA, Yang N, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Efficient generation of dopaminergic induced neuronal cells with 
            midbrain characteristics.
  JOURNAL   Stem Cell Reports. 2021 Jul 13;16(7):1763-1776. doi: 
            10.1016/j.stemcr.2021.05.017. Epub 2021 Jun 24.
  PUBMED    34171286
REFERENCE   2  (bases 1 to 11296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2021.05.017"; journalName: "Stem Cell Reports"; 
            date: "2021-07-13- 13"; volume: "16"; issue: "7"; pages: "1763-1776"
COMMENT     Sequence Label: PiggyBac-TetO-En1-t2a-Foxa2-hygro
FEATURES             Location/Qualifiers
     source          1..11296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..105
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     97..114
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     98..114
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   244..306
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(747..765,766..788,789..807,808..830,831..849,850..872,
                     873..891,892..914,915..933,934..956,957..975,976..998,
                     999..1017)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        1050..1088
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1085..1109
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1188..1197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2421..2474
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2637..2646
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3905..3922)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3911..4135
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4203..5381
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4434..5372
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5329..5349
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(5386..6003,6004..6009,6010..6045,6046..6048,6049..6084,
                     6085..6087,6088..6123,6124..6129)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             6136..6189
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6196..7221
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6196..7221
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7236..7357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7273..7292)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7327..7346
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8120..8154
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8645..8661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8645..8661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8658..8680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8669..8685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8693..8699,8700..8717,8718..8723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8738..8759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8876..8893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9047..9635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9127..9146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9806..10597,10598..10666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10429..10448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10667..10771)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10797..11252)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10934..10955)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11146..11165
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
       61 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
      121 gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc agactcgcgg tgcaaatgtg
      181 ttttacagcg tgatggagca gatgaagatg ctcgacacgc tgcagaacac gcagctagat
      241 taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgt gtaaaattga
      301 cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat agatattaag
      361 ttttattata tttacactta catactaata ataaattcaa caaacaattt atttatgttt
      421 atttatttat taaaaaaaac aaaaactcaa aatttcttct ataaagtaac aaaactttta
      481 tgagggacag ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg
      541 ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc
      601 cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga
      661 acgcttctcg ctgctctttg agcctgcaga cacctggggg gatacgggga aaaggcctcc
      721 acggccacta gtctcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      781 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
      841 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
      901 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
      961 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     1021 gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa
     1081 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     1141 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattcgcc accatggaag
     1201 aacagcagcc ggagcctaaa agtcagcgcg actcgggcct cggcgcggtg gcagcggcgg
     1261 ccccgagcgg cctcagtctg agtctgagcc caggagccag cggcagcagc ggcagcgatg
     1321 gagacagcgt gccggtgtcc ccgcagccag cgcccccgtc gcctcctgcg gcaccctgtc
     1381 tgccgcccct ggcccatcac ccgcacctcc ccccgcatcc cccgcccccg ccgccgccgc
     1441 cgccgccgcc accgcagcat ctcgcggcgc ctgctcacca gccgcagccc gcggcccagc
     1501 tgcaccgcac caccaacttt ttcatcgata acatcctaag gcccgatttc ggttgcaaaa
     1561 aggaacagcc cctgcctcag ctcctggtgg cttcggctgc agccggagga ggcgcagcag
     1621 caggaggagg aagccgcgtg gagcgtgacc gaggccagac tggtgcaggt agagaccccg
     1681 ttcactctct gggcacacga gcttcggggg ctgcctcgct cttgtgtgct ccagatgcga
     1741 actgtggccc acccgacggc tcccagcccg ccaccgctgt cggcgccggc gcatccaaag
     1801 ccgggaaccc ggctgctgcg gcggccgcgg ccgcagcagc ggctgcagcg gcagtggcgg
     1861 cagcggcggc agcagcctcg aagccctcgg acagtggcgg tggtagtgga ggcaacgcgg
     1921 ggagtcccgg ggcgcagggc gccaagttcc cggaacacaa ccctgcgatc ctactcatgg
     1981 gttcggctaa cggtgggccg gtggtcaaga ctgactcaca gcaaccccta gtgtggcccg
     2041 cctgggtcta ctgcacacgc tattcggacc gtccgtcctc tggtccacgc accaggaagc
     2101 taaagaagaa aaagaacgag aaggaagaca agcggccgcg gacggcgttc acggccgagc
     2161 agctgcagag actcaaggcg gagttccagg caaaccgcta tatcacggag cagcggcgac
     2221 agaccctcgc ccaggagctc agcctgaatg agtcccagat caagatctgg ttccaaaaca
     2281 agcgtgccaa gatcaagaaa gccacaggca tcaagaacgg cctggcgctg cacctcatgg
     2341 cccagggact gtacaaccac tctaccacca cggttcagga caaagacgag agcgagggat
     2401 ccatggctag cggctccggc gagggcaggg gaagtcttct aacatgcggg gacgtggagg
     2461 aaaatcccgg cccagcatgc tctagagcca ccatgctggg agcggtgaag atggaagggc
     2521 acgagccgtc cgactggagc agctactatg cagagcccga gggctactcc tccgtgagca
     2581 acatgaacgc cggcctgggg atgaacggca tgaacacgta catgagcatg tcggcggccg
     2641 ccatgggcag cggctcgggc aacatgagcg cgggctccat gaacatgtcg tcgtacgtgg
     2701 gcgctggcat gagcccgtcc ctggcgggga tgtcccccgg cgcgggcgcc atggcgggca
     2761 tgggcggctc ggccggggcg gctggcgtgg cgggcatggg gccgcacttg agtcccagcc
     2821 tgagcccgct cggggggcag gcggccgggg ccatgggcgg cctggccccc tacgccaaca
     2881 tgaactccat gagccccatg tacgggcagg cgggcctgag ccgcgcccgc gaccccaaga
     2941 cctacaggcg cagctacacg cacgcaaagc cgccctactc gtacatctcg ctcatcacca
     3001 tggccatcca gcagagcccc aacaagatgc tgacgctgag cgagatctac cagtggatca
     3061 tggacctctt ccccttctac cggcagaacc agcagcgctg gcagaactcc atccgccact
     3121 cgctctcctt caacgactgt ttcctgaagg tgccccgctc gcccgacaag cccggcaagg
     3181 gctccttctg gaccctgcac cctgactcgg gcaacatgtt cgagaacggc tgctacctgc
     3241 gccgccagaa gcgcttcaag tgcgagaagc agctggcgct gaaggaggcc gcaggcgccg
     3301 ccggcagcgg caagaaggcg gccgccgggg cccaggcctc acaggctcaa ctcggggagg
     3361 ccgccgggcc ggcctccgag actccggcgg gcaccgagtc gcctcactcg agcgcctccc
     3421 cgtgccagga gcacaagcga gggggcctgg gagagctgaa ggggacgccg gctgcggcgc
     3481 tgagcccccc agagccggcg ccctctcccg ggcagcagca gcaggccgcg gcccacctgc
     3541 tgggcccgcc ccaccacccg ggcctgccgc ctgaggccca cctgaagccg gaacaccact
     3601 acgccttcaa ccacccgttc tccatcaaca acctcatgtc ctcggagcag cagcaccacc
     3661 acagccacca ccaccaccag ccccacaaaa tggacctcaa ggcctacgaa caggtgatgc
     3721 actaccccgg ctacggttcc cccatgcctg gcagcttggc catgggcccg gtcacgaaca
     3781 aaacgggcct ggacgcctcg cccctggccg cagatacctc ctactaccag ggggtgtact
     3841 cccggcccat tatgaactcc tcttagttaa ttaagctagc cctaggctag agctcgctga
     3901 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3961 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4021 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4081 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4141 gaggcggaaa gaaccagctg gggctcgact agagcttgcg gaacccttcg cggccgccgt
     4201 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     4261 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     4321 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4381 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     4441 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     4501 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     4561 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     4621 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     4681 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     4741 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     4801 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     4861 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     4921 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     4981 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     5041 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     5101 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     5161 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     5221 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     5281 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     5341 cagtggttca aagttttttt cttccatttc aggtgtcgtg agaccatgtc tagactggac
     5401 aagagcaaag tcataaacgg cgctctggaa ttactcaatg gagtcggtat cgaaggcctg
     5461 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     5521 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     5581 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     5641 ttccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     5701 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     5761 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     5821 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     5881 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     5941 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     6001 ggcgggccgg ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     6061 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     6121 ctccccgggg gatccgaggg caggggaagt cttctaacat gcggggacgt ggaggaaaat
     6181 cccggcccag ctagcatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     6241 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
     6301 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
     6361 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
     6421 gaagtgcttg acattgggga atttagcgag agcctgacct attgcatctc ccgccgtgca
     6481 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
     6541 gcggaggcca tggatgcgat cgctgcggct gatcttagcc agacgagcgg gttcggccca
     6601 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
     6661 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     6721 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     6781 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     6841 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     6901 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     6961 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     7021 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     7081 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     7141 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     7201 cgtccgaggg caaaggaata ggtcgacgtt aactaaactt gtttattgca gcttataatg
     7261 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7321 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggaat tgactcaaat
     7381 gatgtcaatt agtctatcag aagctcatct ggtctccctt ccgggggaca agacatccct
     7441 gtttaatatt taaacagcag tgttcccaaa ctgggttctt atatcccttg ctctggtcaa
     7501 ccaggttgca gggtttcctg tcctcacagg aacgaagtcc ctaaagaaac agtggcagcc
     7561 aggtttagcc ccggaattga ctggattcct tttttagggc ccattggtat ggctttttcc
     7621 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     7681 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     7741 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     7801 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctgatat ctataacaag
     7861 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
     7921 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
     7981 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
     8041 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
     8101 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaatc
     8161 tagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg
     8221 cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca
     8281 tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt
     8341 accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact gttcgttcag
     8401 gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat ggtcagcccg
     8461 aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag
     8521 attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta tcctggctgg
     8581 atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc ttggcgtaat
     8641 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8701 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8761 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9781 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9841 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9901 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9961 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10021 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10081 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10141 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10201 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10261 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10321 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10381 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10441 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10501 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10561 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10621 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10681 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10741 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
    10801 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
    10861 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
    10921 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
    10981 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
    11041 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
    11101 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
    11161 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
    11221 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
    11281 ttgggaaggg cgatcg
//
