LOCUS       sequence-359751-        4866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Secreted Luciferase reporter for AhR activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Degrelle SA, Ferecatu I, Fournier T
  TITLE     Novel fluorescent and secreted transcriptional reporters for 
            quantifying activity of the xenobiotic sensor aryl hydrocarbon 
            receptor (AHR).
  JOURNAL   Environ Int. 2022 Sep 24;169:107545. doi: 
            10.1016/j.envint.2022.107545.
  PUBMED    36179647
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Environ 
            Int."; date: "2022-09-24"; volume: "169:107545. doi"; pages: " 
            10.1016/j.envint.2022.107545"
COMMENT     Sequence Label: XRE-pNL1.3[secNLuc]
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..46
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     31..47
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1859..2371
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     polyA_signal    2420..2541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2431..2450)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2485..2504
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2789..2806)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2960..3548)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3040..3059)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3748..4539,4540..4608))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4713..4761
                     /note="synthetic polyadenylation signal"
     misc_feature    4775..4866
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4815..4834
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccg ggccccccct cgaggtcgac ggtatcgata agcttctggc
       61 acatagtagt tgcttggtaa atatttgtgc aatgcacgag gccgcatatg accggaatgg
      121 gaggtgaggg gattattttc tggcctggac cagcgacgga tggaggtgcc accgggttgg
      181 ggagcacgtc ggggatggcg cgtaacgatg ttagctgggg ccaggttgag ctaggcacgc
      241 aaatacaact ttttttttcc tggaaaccct gtaacaggaa ggttccggag ggcgggacag
      301 cgtcggaggc aggcagctag gccatgccaa atggcactgg ggcttcgtgt cgtgccacag
      361 gcgtggaccg aaaatgcgga cacatgcagg ctgcctctcc tcgcaggcag aagccacacg
      421 cagacctaga ccctttgcac cgcatcccct tattcaatcg cgcacccgcc acccttcgac
      481 agttcctctc cctccacccc aaccccacgc cgcgcgcgag gctggccctt taagagcccc
      541 gccccgactc cctcccccct cgcgtgactg cgagcccccg cgccgggccg gggaatgggt
      601 cggctgggtg gctgcgcggg cctccggtcc ttctcacgca acgcctgggc accgcgcctc
      661 cgggccaggt ggggcgggga cgggccgcct gacctctgcc ccctagaggg atgtcgccgg
      721 cgcacgcaag ctagccgggg gtagggtggg ggctccgcgc caggtgcccc ctccgtggtc
      781 cctgggcccg agtctttccg tggccccccg ccgccggatt tctgtgctct gccaatcaaa
      841 gcactagcca ccccgggagc caagagggac cctcaagggc cggtgggtcc tggctggagg
      901 gaccgcgcgt tgcaatcagc actaaggcga tcctagaggc tgcgaggagc cgctagtgag
      961 cgctcagcga gcctgcccct tcgccatcca ttccgatcct tcaatcaaga ggcgcgaacc
     1021 tcagctagtc gcccgggctc tgggggacag gtccagcccc gcggcgcctc tggccttccg
     1081 gcccccgtga cctcagggct ggggtcgcag cgcttctcac gcgagccggg actcagtaac
     1141 cccgggaaga aggtcaccac ggggcagccc cgcccccgcc tgccgagtcc tggtaggctg
     1201 tagcgctggg gaggcatctg cacgcccagc gttccagtgg gtgcaaaaat gacgaagagg
     1261 agtccccgcg ccccaggatg gagcttcccg taccctctct tcgggctgtc ctgggacttc
     1321 tccctcaagc cccctcctcg gctgggttct gcactgccct tgggacgcct tggaattggg
     1381 acttccaggt gttcccagcc ctcacccctc tatgtacagg caccgagatg tgtcccatag
     1441 tgggttcttg cccacccgac cccccacccc cgccgccctc cgccaccttt ctctccaatc
     1501 ccagagagac cagcccggtt caggctgctt ctccctccat ctcagctcgc tccagggaag
     1561 gaggcgtggc cacacgtaca agcccgccta taaaggtggc agtgccttca ccctcaccct
     1621 gaaggtgaca gttccttgga accttccctg atccttgtga tcccaggctc caagagtcca
     1681 cccttcccag ctgagctcgc tagcctcgag gatatcaaga tctggcctcg gcggccaagc
     1741 ttggcaatcc ggtactgttg gtaaagccac catgaactcc ttctccacaa gcgccttcgg
     1801 tccagttgcc ttctccctgg gcctgctcct ggtgttgcct gctgccttcc ctgccccagt
     1861 cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca acctggacca
     1921 agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc
     1981 gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat
     2041 cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt
     2101 gtaccctgtg gatgatcatc actttaaggt gatcctgcac tatggcacac tggtaatcga
     2161 cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca tcgccgtgtt
     2221 cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa ttatcgacga
     2281 gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg gagtgaccgg
     2341 ctggcggctg tgcgaacgca ttctggcgta aggccgcgac tctagagtcg gggcggccgg
     2401 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     2461 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2521 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2581 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     2641 aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     2701 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     2761 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3361 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3481 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
     3721 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
     3781 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
     3841 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
     3901 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
     3961 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
     4021 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
     4081 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
     4141 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
     4201 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
     4261 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
     4321 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
     4381 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
     4441 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
     4501 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
     4561 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
     4621 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
     4681 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
     4741 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
     4801 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
     4861 ttctct
//
