LOCUS       sequence-359752-        6157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for AhR activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Degrelle SA, Ferecatu I, Fournier T
  TITLE     Novel fluorescent and secreted transcriptional reporters for 
            quantifying activity of the xenobiotic sensor aryl hydrocarbon 
            receptor (AHR).
  JOURNAL   Environ Int. 2022 Sep 24;169:107545. doi: 
            10.1016/j.envint.2022.107545.
  PUBMED    36179647
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Environ 
            Int."; date: "2022-09-24"; volume: "169:107545. doi"; pages: " 
            10.1016/j.envint.2022.107545"
COMMENT     Sequence Label: XRE-H2B-eGFP
FEATURES             Location/Qualifiers
     source          1..6157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1781..2158
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      2171..2180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2177..2179,2180..2182,2183..2896)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2177..2179,2180..2182,2183..2893)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2222..2243)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2483..2502)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2830..2851
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3019..3140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3056..3075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3110..3129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3147..3602)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3284..3305)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3496..3515
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3629..3733
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3735..4092
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3735..3926
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3758..3778)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3943..4078
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4127..4921
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4127..4921
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4181..4200)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4791..4810
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5109..5128)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5153..5200
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5529..6117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6018..6037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
       61 ttaccgccat gcattagtta ttaatgcttc tggcacatag tagttgcttg gtaaatattt
      121 gtgcaatgca cgaggccgca tatgaccgga atgggaggtg aggggattat tttctggcct
      181 ggaccagcga cggatggagg tgccaccggg ttggggagca cgtcggggat ggcgcgtaac
      241 gatgttagct ggggccaggt tgagctaggc acgcaaatac aacttttttt ttcctggaaa
      301 ccctgtaaca ggaaggttcc ggagggcggg acagcgtcgg aggcaggcag ctaggccatg
      361 ccaaatggca ctggggcttc gtgtcgtgcc acaggcgtgg accgaaaatg cggacacatg
      421 caggctgcct ctcctcgcag gcagaagcca cacgcagacc tagacccttt gcaccgcatc
      481 cccttattca atcgcgcacc cgccaccctt cgacagttcc tctccctcca ccccaacccc
      541 acgccgcgcg cgaggctggc cctttaagag ccccgccccg actccctccc ccctcgcgtg
      601 actgcgagcc cccgcgccgg gccggggaat gggtcggctg ggtggctgcg cgggcctccg
      661 gtccttctca cgcaacgcct gggcaccgcg cctccgggcc aggtggggcg gggacgggcc
      721 gcctgacctc tgccccctag agggatgtcg ccggcgcacg caagctagcc gggggtaggg
      781 tgggggctcc gcgccaggtg ccccctccgt ggtccctggg cccgagtctt tccgtggccc
      841 cccgccgccg gatttctgtg ctctgccaat caaagcacta gccaccccgg gagccaagag
      901 ggaccctcaa gggccggtgg gtcctggctg gagggaccgc gcgttgcaat cagcactaag
      961 gcgatcctag aggctgcgag gagccgctag tgagcgctca gcgagcctgc cccttcgcca
     1021 tccattccga tccttcaatc aagaggcgcg aacctcagct agtcgcccgg gctctggggg
     1081 acaggtccag ccccgcggcg cctctggcct tccggccccc gtgacctcag ggctggggtc
     1141 gcagcgcttc tcacgcgagc cgggactcag taaccccggg aagaaggtca ccacggggca
     1201 gccccgcccc cgcctgccga gtcctggtag gctgtagcgc tggggaggca tctgcacgcc
     1261 cagcgttcca gtgggtgcaa aaatgacgaa gaggagtccc cgcgccccag gatggagctt
     1321 cccgtaccct ctcttcgggc tgtcctggga cttctccctc aagccccctc ctcggctggg
     1381 ttctgcactg cccttgggac gccttggaat tgggacttcc aggtgttccc agccctcacc
     1441 cctctatgta caggcaccga gatgtgtccc atagtgggtt cttgcccacc cgacccccca
     1501 cccccgccgc cctccgccac ctttctctcc aatcccagag agaccagccc ggttcaggct
     1561 gcttctccct ccatctcagc tcgctccagg gaaggaggcg tggccacacg tacaagcccg
     1621 cctataaagg tggcagtgcc ttcaccctca ccctgaaggt gacagttcct tggaaccttc
     1681 cctgatcctt gtgatcccag gctccaagag tccacccttc ccagctgagc tcagatctcg
     1741 agctcaagct tcgaattctg cagtcgacgg taccgccacc atgccagagc cagcgaagtc
     1801 tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg
     1861 caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa
     1921 gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt
     1981 gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg
     2041 ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt
     2101 ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaagga
     2161 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2221 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2281 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2341 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2401 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2461 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2521 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2581 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2641 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2701 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2761 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     2821 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2881 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     2941 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3001 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3061 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3121 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3181 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3241 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3301 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3361 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3421 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3481 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3541 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3601 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3661 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3721 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3781 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3841 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3901 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3961 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4021 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4081 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4141 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4201 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4261 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4321 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4381 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4441 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4501 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4561 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4621 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4681 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4741 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4801 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4861 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4921 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4981 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5041 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     5101 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5161 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5221 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5281 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5341 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5401 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5461 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5521 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5581 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5641 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5701 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5761 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5821 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5881 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5941 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6001 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6061 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6121 gccagcaacg cggccttttt acggttcctg gcctttt
//
