LOCUS       sequence-35989-e         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Hageman J, Kampinga HH
  TITLE     Computational analysis of the human HSPH/HSPA/DNAJ family and 
            cloning of a human HSPH/HSPA/DNAJ expression library.
  JOURNAL   Cell Stress Chaperones. 2009 Jan;14(1):1-21. doi: 
            10.1007/s12192-008-0060-2. Epub 2008 Aug 7.
  PUBMED    18686016
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12192-008-0060-2"; journalName: "Cell Stress Chaperones"; 
            date: "2009-01"; volume: "14"; issue: "1"; pages: "1-21"
COMMENT     Sequence Label: pcDNA5/FRT/TO V5 DNAJB5
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             187..228
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttgaattc
      181 accatgggta agcctatccc taaccctctc ctcggtctcg attctacggg atccactagt
      241 ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgcaccat gggaaaagat
      301 tattacaaga ttcttgggat cccatcgggg gccaacgagg atgagatcaa gaaagcctac
      361 cggaagatgg ccttgaagta ccacccagac aagaataaag aacccaacgc tgaggagaag
      421 tttaaggaga ttgcagaggc ctatgatgtg ctaagtgacc ccaagaaacg gggcctgtat
      481 gaccagtatg gggaggaagg cctgaagacc ggcggtggca catcaggtgg ctccagtggc
      541 tcctttcact acacctttca tggggacccc catgccacct ttgcctcctt ctttggtggc
      601 tccaacccct tcgatatctt ctttgccagc
//
