LOCUS       sequence-359998-        9192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  VDR - Vitamin D receptor gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE14
FEATURES             Location/Qualifiers
     source          1..9192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2024..2055
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2082..2091
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2088..3737
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3741..3860
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3952..4540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4005..4025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4423..4434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4555..4871
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4722..4857
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4922..4924,4925..4927,4928..5641)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4967..4988)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5228..5247)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5575..5596
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5713..5946
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6018..6139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6055..6074)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6109..6128
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6179..6314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6334..6353)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6335..6353)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6363..6380)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6363..6379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6372..6394)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6521..6976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6608..6627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6818..6839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7002..7106
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7107..7175,7176..7967)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7325..7344)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8138..8726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8627..8646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8880..8897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9014..9035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9050..9055,9056..9073,9074..9080)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9088..9104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9093..9115
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9112..9128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9112..9128
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9147..9167
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9149..9167
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcttcag gtcaaggagg tcaaggaggt caaggaggtc
     1981 agatccgaga acagatctgg cctcggcggc caagcttaga cactagaggg tatataatgg
     2041 aagctcgact tccagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca
     2101 aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc
     2161 agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
     2221 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
     2281 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
     2341 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
     2401 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
     2461 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
     2521 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
     2581 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
     2641 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
     2701 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
     2761 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
     2821 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
     2881 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
     2941 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     3001 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     3061 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     3121 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     3181 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     3241 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     3301 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     3361 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     3421 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     3481 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     3541 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     3601 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     3661 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     3721 gcggcaagat cgccgtgaat tctcacggct tccctcccga ggtggaggag caggccgccg
     3781 gcaccctgcc catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg
     3841 ccagcgccag gatcaacgtc taaggccgcg actctagaac gcgtcactgc ggctgggttt
     3901 ttgtctgcgt agactacaga catcagcgcg taccggttag taatgatcga caatcaacct
     3961 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4021 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4081 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4141 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4201 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4261 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4321 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4381 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4441 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4501 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggcgatgg taccggtgtg
     4561 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4621 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4681 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4801 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4861 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacctag
     4921 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     4981 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5041 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5101 cctcgtgacc accttcacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     5161 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5221 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5281 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5341 caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa
     5401 cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc
     5461 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5521 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5581 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     5641 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5701 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5761 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5821 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5881 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5941 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6001 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6061 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6121 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6241 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6301 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6361 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6421 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6481 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6541 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6601 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6661 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6721 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6781 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6841 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6901 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6961 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7021 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7081 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7141 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7201 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7261 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7321 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7381 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7441 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7501 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7561 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7621 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7681 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7741 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7801 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7861 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7921 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7981 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8041 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8101 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8161 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8221 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8281 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8341 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8401 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8461 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8521 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8581 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8641 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8701 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8761 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8821 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8881 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8941 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9001 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9061 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9121 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9181 agctgcaagc tt
//
