LOCUS       sequence-360001-        9308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STAT5 - Stat5 gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE6
FEATURES             Location/Qualifiers
     source          1..9308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2138..2169
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2196..2205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2202..3851
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3855..3974
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    4068..4656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4121..4141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4539..4550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4671..4987
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4838..4973
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5038..5040,5041..5043,5044..5757)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5083..5104)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5344..5363)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5691..5712
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5829..6062
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6134..6255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6171..6190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6225..6244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6295..6430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6450..6469)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6451..6469)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6479..6496)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6479..6495)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6488..6510)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6637..7092
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6724..6743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6934..6955
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7118..7222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7223..7291,7292..8083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7441..7460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8254..8842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8743..8762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8996..9013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9130..9151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9166..9171,9172..9189,9190..9196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9204..9220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9209..9231
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9228..9244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9228..9244
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9263..9283
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9265..9283
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcgcttt tagatttcta ggaattcaat ccttttagat
     1981 ttctaggaat tcaatccttt agatttctag gaattcaatc cttttagatt tctaggaatt
     2041 caatccttta gatttctagg aattcaatcc ttttagattt ctaggaattc aatccgatcc
     2101 gagaacagat ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc
     2161 gacttccagc ttggcaatcc ggtactgttg gtaaagccac catggaagat gccaaaaaca
     2221 ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc
     2281 acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata
     2341 tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta
     2401 tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc
     2461 agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg
     2521 acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat
     2581 tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac
     2641 aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct
     2701 tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg
     2761 accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg
     2821 gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct
     2881 tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct
     2941 tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc
     3001 gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc
     3061 tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa
     3121 gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg
     3181 tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca
     3241 gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc
     3301 ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc
     3361 gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg
     3421 ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg
     3481 acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct
     3541 accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg
     3601 ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc
     3661 tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta
     3721 caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga
     3781 ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca
     3841 agatcgccgt gaattctcac ggcttccctc ccgaggtgga ggagcaggcc gccggcaccc
     3901 tgcccatgag ctgcgcccag gagagcggca tggatagaca ccctgctgct tgcgccagcg
     3961 ccaggatcaa cgtctaaggc cgcgactcta gaacgcgtca ctgcggctnn gctkmanggg
     4021 nttgacggac tacagacatc agcgcgtacc ggttagtaat gatcgacaat caacctctgg
     4081 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4141 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4201 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4261 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4321 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4381 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4441 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     4501 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4561 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4621 agacgagtcg gatctccctt tgggccgcct ccccgcctgg cgatggtacc ggtgtggaaa
     4681 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4741 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4801 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4861 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4921 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     4981 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acctagcatg
     5041 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     5101 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     5161 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     5221 gtgaccacct tcacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     5281 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     5341 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5401 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5461 ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc
     5521 atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5581 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5641 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5701 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaggt
     5761 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5821 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     5881 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5941 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6001 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6061 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6121 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6181 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6241 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     6301 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6361 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6421 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     6481 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6541 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6601 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     6661 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6721 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6781 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6841 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6901 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6961 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7021 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7081 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7141 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7201 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7261 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7321 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7381 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7441 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7501 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7561 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7621 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7681 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7741 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7801 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7861 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7921 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7981 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8041 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8101 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8161 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8221 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8281 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8341 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8401 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8461 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8521 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8581 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8641 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8701 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8761 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8821 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8881 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8941 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9001 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9061 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9121 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     9181 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     9241 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     9301 gcaagctt
//
