LOCUS       sequence-360009-        9281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STAT3 - Sis-inducible element (SIE) gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE59
FEATURES             Location/Qualifiers
     source          1..9281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2113..2144
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2171..2180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2177..3826
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3830..3949
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    4041..4629
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4094..4114)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4512..4523)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4644..4960
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4811..4946
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5011..5013,5014..5016,5017..5730)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5056..5077)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5317..5336)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5664..5685
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5802..6035
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6107..6228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6144..6163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6198..6217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6268..6403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6423..6442)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6424..6442)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6452..6469)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6452..6468)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6461..6483)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6610..7065
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6697..6716)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6907..6928
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7091..7195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7196..7264,7265..8056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7414..7433)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8227..8815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8716..8735
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8969..8986
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9103..9124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9139..9144,9145..9162,9163..9169)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9177..9193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9182..9204
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9201..9217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9201..9217
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9236..9256
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9238..9256
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcagctt catttcccgt aaatcgtcga agcttcattt
     1981 cccgtaaatc gtcgaagctt catttcccgt aaatcgtcga agcttcattt cccgtaaatc
     2041 gtcgaagctt catttcccgt aaatcgtcga gatccgagaa cagatctggc ctcggcggcc
     2101 aagcttagac actagagggt atataatgga agctcgactt ccagcttggc aatccggtac
     2161 tgttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta
     2221 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
     2281 ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga
     2341 gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa
     2401 ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc
     2461 cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct
     2521 gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa
     2581 gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa
     2641 gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg
     2701 cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat
     2761 catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc
     2821 ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac
     2881 cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta
     2941 cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg
     3001 cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt
     3061 cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg
     3121 cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg
     3181 catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg
     3241 ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga
     3301 cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc
     3361 catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga
     3421 cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt
     3481 ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga
     3541 gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga
     3601 cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga
     3661 gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg
     3721 tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat
     3781 ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgaatt ctcacggctt
     3841 ccctcccgag gtggaggagc aggccgccgg caccctgccc atgagctgcg cccaggagag
     3901 cggcatggat agacaccctg ctgcttgcgc cagcgccagg atcaacgtct aaggccgcga
     3961 ctctagaacg cgtcactgcg gctgmyyrts gwsscttcgt gactacagac atcagcgcgt
     4021 accggttagt aatgatcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4081 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4141 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4201 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4261 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4321 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4381 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     4441 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4501 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4561 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4621 cctccccgcc tggcgatggt accggtgtgg aaagtcccca ggctccccag caggcagaag
     4681 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4741 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4801 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4861 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     4921 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     4981 atccattttc ggatctgatc agcacctagc atggtgagca agggcgagga gctgttcacc
     5041 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     5101 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     5161 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag
     5221 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     5281 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     5341 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5401 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
     5461 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
     5521 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5581 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     5641 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5701 actctcggca tggacgagct gtacaagtaa ggtaccttta agaccaatga cttacaaggc
     5761 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5821 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5881 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5941 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6001 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6061 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     6121 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6181 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6241 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6301 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6361 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6421 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6481 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6541 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6601 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6661 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6721 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6781 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6841 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6901 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6961 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7021 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     7081 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7141 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7201 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7261 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7321 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7381 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7441 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7501 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7561 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7621 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7681 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7741 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7801 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7861 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7921 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7981 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8041 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8101 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8161 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8221 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8281 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8341 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8401 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8461 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8521 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8581 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8641 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8701 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8761 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8821 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8881 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8941 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9001 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9061 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9121 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9181 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     9241 taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
