LOCUS       sequence-360013-        9211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C/EBPa - CEBPA gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE54
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2043..2074
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2101..2110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2107..3756
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3760..3879
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3971..4559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4024..4044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4442..4453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4574..4890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4741..4876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4941..4943,4944..4946,4947..5660)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4986..5007)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5247..5266)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5594..5615
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5732..5965
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6037..6158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6074..6093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6128..6147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6198..6333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6353..6372)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6354..6372)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6382..6399)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6382..6398)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6391..6413)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6540..6995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6627..6646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6837..6858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7021..7125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7126..7194,7195..7986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7344..7363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8157..8745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8646..8665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8899..8916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9033..9054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9069..9074,9075..9092,9093..9099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9107..9123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9112..9134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9131..9147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9131..9147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9166..9186
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9168..9186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcatgca atacccgtgc aatccccgtg caatccccat
     1981 gcaatacccg tgcaataccc gatccgagaa cagatctggc ctcggcggcc aagcttagac
     2041 actagagggt atataatgga agctcgactt ccagcttggc aatccggtac tgttggtaaa
     2101 gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa
     2161 gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc
     2221 accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag
     2281 atgagcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc
     2341 gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc
     2401 ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg
     2461 ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac
     2521 gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac
     2581 cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag
     2641 tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt
     2701 agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga
     2761 ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc
     2821 agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc
     2881 ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa
     2941 gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc
     3001 actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg
     3061 ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag
     3121 ggctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag
     3181 cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc
     3241 ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg
     3301 agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg
     3361 cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg
     3421 aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg
     3481 ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc
     3541 gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc
     3601 gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc
     3661 gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt
     3721 ctcattaagg ccaagaaggg cggcaagatc gccgtgaatt ctcacggctt ccctcccgag
     3781 gtggaggagc aggccgccgg caccctgccc atgagctgcg cccaggagag cggcatggat
     3841 agacaccctg ctgcttgcgc cagcgccagg atcaacgtct aaggccgcga ctctagaacg
     3901 cgtcactgcg gcttactgat gtctgaacct gactacagac atcagcgcgt accggttagt
     3961 aatgatcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4021 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4081 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4141 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4201 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4261 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4321 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4381 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4441 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4501 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4561 tggcgatggt accggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4621 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4681 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4741 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4801 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     4861 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     4921 ggatctgatc agcacctagc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4981 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     5041 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     5101 tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcgccc
     5161 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5221 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     5281 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     5341 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca
     5401 ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg
     5461 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5521 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5581 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5641 tggacgagct gtacaagtaa ggtaccttta agaccaatga cttacaaggc agctgtagat
     5701 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5761 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5821 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5881 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5941 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6001 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     6061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6121 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     6181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6301 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     6361 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     6421 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     6481 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     6541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6661 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6721 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6781 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6841 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6901 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6961 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     7021 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7081 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7141 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7201 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7261 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7321 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7381 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7441 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7501 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     7561 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     7621 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     7681 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7741 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7801 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7861 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7921 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7981 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8041 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8101 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8161 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8221 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8281 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8341 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8401 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8461 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     8521 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     8581 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     8641 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     8701 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8761 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8821 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8881 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8941 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     9001 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9061 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9121 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     9181 taaagggaac aaaagctgga gctgcaagct t
//
