LOCUS       sequence-360048-        9135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), 
            encoding C-terminal residues 1476-3222, was inserted into the 
            pMT-FLAG vector. Expression in S2 cells by CuSO4 induction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Tie F, Banerjee R, Stratton CA, Prasad-Sinha J, Stepanik V, Zlobin 
            A, Diaz MO, Scacheri PC, Harte PJ
  TITLE     CBP-mediated acetylation of histone H3 lysine 27 antagonizes 
            Drosophila Polycomb silencing.
  JOURNAL   Development. 2009 Sep;136(18):3131-41. doi: 10.1242/dev.037127.
  PUBMED    19700617
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2009-09"; volume: "136"; issue: 
            "18"; pages: "3131-41"
COMMENT     Sequence Label: pMT-FLAG-CBP-Delta-N
FEATURES             Location/Qualifiers
     source          1..9135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        411..833
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             850..873
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6112..6129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6921..6938)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6921..6937)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6930..6952)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7146..7165)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7265..7287
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7325..7343)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7411..7515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7516..7584,7585..8376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8547..9135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9036..9055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac cgttgcagga
      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagcttt gcatcccata
      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggaattc
      841 gagctcatgg actacaagga cgacgatgac aaggctagca atccactgct cctcatgtct
      901 ggaggaacgg caggaggcgg aacgggagca acgaccacca catcgacatc ctcgagcagt
      961 cgcatgatga gcagctccag cagtctctcc tcacagatgg ctgccctgga ggctgcggcg
     1021 cgagacaacg acgatgagac gccctcgcca tccggcgaga atacgaacgg cagtggtggc
     1081 agtggaaatg ccggcggtat ggcctccaag ggcaaactgg actccattaa gcaagatgat
     1141 gatatcaaga aggagtttat ggatgacagc tgtggcggaa ataacgatag ctcgcagatg
     1201 gattgctcga cgggtggtgg caagggcaag aatgtgaaca acgacggaac aagcatgatc
     1261 aaaatggaga tcaagacgga ggatggactc gatggcgagg taaagatcaa aacggaggcc
     1321 atggatgtgg acgaggctgg aggatcgaca gccggagagc atcatggcga aggtggcggc
     1381 ggcagtggtg ttggcggcgg taaggataac ataaatggtg cgcacgatgg cggagcgaca
     1441 ggcggtgctg tggatataaa acccaagacg gagacgaaac cactcgtacc ggagccactg
     1501 gcacccaatg caggtgacaa gaaaaagaag tgccaattca atcccgagga actgcgcacc
     1561 gctctcctgc caacgctaga gaagctctac aggcaggagc ccgaatccgt gccctttcgc
     1621 tacccagttg atccccaggc gctgggcata cctgattact ttgaaatcgt aaagaagccc
     1681 atggacctgg gcactatacg caccaacatc cagaatggaa agtacagtga tccctgggaa
     1741 tatgtggacg acgtttggct gatgttcgac aatgcctggc tgtataatcg caaaacatcg
     1801 cgggtctatc gctattgcac aaagctttcc gaagtctttg aggcggagat tgatcctgtg
     1861 atgcaggcac tgggatattg ctgcggcagg aagtacacat tcaatccaca ggtgctatgc
     1921 tgctacggca agcagctctg cacgattccg cgggatgcca agtactacag ctaccagaac
     1981 agtctaaagg aatacggtgt cgcctcaaat agatacacct actgccaaaa gtgctttaac
     2041 gacatccagg gcgatacggt cacactgggc gacgatccac tgcaatcgca aacccaaatc
     2101 aaaaaggatc agttcaagga gatgaagaac gatcacctcg aactggagcc gtttgtcaat
     2161 tgccaggagt gcggacgcaa acagcaccaa atctgcgtac tctggctgga ttctatctgg
     2221 cccggtggct tcgtgtgcga taactgcctg aaaaagaaga actcaaagcg gaaggagaac
     2281 aagttcaatg cgaaacgcct gcccaccacc aagctgggcg tgtacataga gacgcgggtg
     2341 aataatttcc tcaagaagaa ggaggctggt gccggcgagg tgcacattcg tgtggtcagc
     2401 tcatcggaca agtgtgtaga ggtgaagccc ggcatgcgtc gacgattcgt tgagcagggc
     2461 gagatgatga acgagttccc ataccgagcc aaagcgctct ttgccttcga ggaggtggat
     2521 ggcatcgatg tgtgcttctt tggcatgcac gttcaggagt atggatccga gtgcccggcg
     2581 ccgaatacgc ggcgtgtgta tattgcctat ttggattccg ttcatttctt ccggccaaga
     2641 cagtaccgta cagcggtata tcacgaaatc ctgctcggct atatggacta cgtgaaacag
     2701 ctgggctaca caatggccca tatctgggcc tgtccgccat ccgagggcga tgactacatc
     2761 tttcactgcc atcccacgga ccagaagata cccaagccca agcgcctgca ggagtggtac
     2821 aaaaagatgc ttgacaaggg aatgatcgag cgcatcatac aggactacaa ggatatcctg
     2881 aagcaggcga tggaggacaa actgggctct gccgcagagc tgccctactt tgagggcgac
     2941 ttctggccca atgtgctgga ggagagcatc aaggaactgg accaggagga ggaagagaag
     3001 cgcaaacagg ccgaggccgc ggaagcagca gctgcggcaa atcttttctc tatcgaggaa
     3061 aatgaagtaa gcggcgatgg caaaaagaag ggccagaaga aggccaaaaa gtcgaacaaa
     3121 tcgaaagcgg cgcagcgtaa gaacagcaaa aagtccaacg aacatcagtc gggcaatgat
     3181 ctctccacaa agatatatgc gaccatggag aagcacaagg aggtcttctt cgttatccgt
     3241 ctgcattcgg cgcagtcggc agctagttta gcgcccatcc aggatcccga tccgctgctc
     3301 acatgcgatc tgatggatgg acgcgatgcc ttcctcacgc tcgcccgcga caagcacttt
     3361 gagttctcgt cgctgcggcg cgcacaattc tccactctgt ccatgttgta tgagctgcat
     3421 aaccagggtc aggacaagtt tgtttacacc tgcaaccact gcaagacggc cgtggagacg
     3481 cgctaccact gtactgtttg tgatgacttc gatctgtgta tcgtgtgcaa ggagaaggtt
     3541 ggccatcagc acaagatgga gaagctcggc ttcgacatcg acgacggctc tgcgctggcg
     3601 gatcacaagc aggctaatcc acaggaggcc cgcaagcaat ccatccagcg ttgcatccaa
     3661 tcgctggcgc acgcctgcca gtgtcgcgat gccaactgcc gcctgccatc gtgccagaag
     3721 atgaagctcg ttgtccagca tacgaagaac tgcaagcgca agcccaacgg aggatgcccc
     3781 atttgcaagc agcttatcgc actctgttgc tatcacgcga agaactgtga ggagcagaag
     3841 tgccccgtgc cgttctgtcc caacatcaag cacaagctca agcagcagca gtcacagcag
     3901 aaattccagc agcagcagtt gctgcgtcgc cgtgtggcgc tcatgtcgcg tacagcagct
     3961 ccagcggctc tgcaaggccc agctgcagta agcggtccga ccgtcgtctc tggaggagtg
     4021 cccgtggtgg gcatgtccgg tgtggcagtt agccaacagg tgatccccgg ccaggcgggt
     4081 atactgcctc caggggcggg tggcatgtcg ccatctaccg tggcagttcc atcgcctgtt
     4141 tcaggaggag cgggagccgg tggaatgggt ggaatgacat caccacatcc gcatcaacca
     4201 ggtataggta tgaaacctgg tggcggtcac tcgccgtctc caaatgtcct acaagtggtg
     4261 aagcaggtcc aggaagaggc agctcgtcag caggtatcgc atggcggtgg cttcggcaag
     4321 ggcgtaccca tggcgccgcc cgtaatgaat cgaccaatgg gcggcgctgg gcccaaccaa
     4381 aatgttgtta atcaacttgg tggcatgggc gttggagttg gaggtgtcgg tggtgttggc
     4441 gtcggaggcg ttggtggagt gggtgttaat caactgaatt cgggtggtgg caatacaccc
     4501 ggtgcaccca tttccggtcc cggaatgaat gtcaatcatc taatgtccat ggatcagtgg
     4561 ggcggtggcg gagccggcgg cggaggtgcc aatcccggcg gtggcaatcc acaagcccgc
     4621 tatgccaaca ataccggcgg catgcgccaa cccacccatg tgatgcaaac gaatctgata
     4681 ccgccgcagc aacagcaaca gatgatgggc ggactgggcg gacccaacca actgggaggt
     4741 ggccaaatgc cagtcggcgg acagcatgga ggaatgggaa tgggcatggg agcaccacca
     4801 atggccggaa ctgttggcgg agtgcgtcca tctcccggag caggaggtgg aggtggaagt
     4861 gcgactgggg gcggtctaaa tacgcaacaa ctcgccctga ttatgcaaaa gattaagaac
     4921 aatcccacca acgagagcaa ccagcacatc cttgccatac taaaacagaa tccgcagatc
     4981 atggcggcga tcatcaagca gcgccagcag tcgcagaaca atgcggcagc gggcggagga
     5041 gcacctggcc caggtggagc cctacagcag cagcaggccg gtaacggacc gcaaaatcct
     5101 caacagcagc agcagcagca gcaacagcaa caggtgatgc agcaacagca gatgcagcac
     5161 atgatgaacc agcagcaggg cggcggcggt ccacagcaga tgaatcccaa ccagcagcag
     5221 caacagcagc aggttaatct catgcagcag cagcaacaag gtggacccgg aggaccaggt
     5281 tctggacttc ccacgcgcat gcccaatatg cccaatgcct tgggtatgct gcagagtctt
     5341 ccgcccaaca tgtcgccagg cgtttctact cagggaggaa tggtgcccaa ccaaaactgg
     5401 aacaagatgc gttacatgca aatgagccag tacccgccac cgtatccgca gcgccagcgt
     5461 ggcccgcaca tgggcggagc gggacctggt cccggccagc aacagttccc cggtggcgga
     5521 ggtggagcgg gcaactttaa tgcgggtggt gctggtggtg caggcggcgt tgtcggtgtg
     5581 ggcggagtgc ccggaggtgc cggcacggtg cccggtggcg atcaatactc gatggcgaat
     5641 gccgcggctg cctccaatat gctgcaacag cagcagggcc aggtgggcgt cggagtgggc
     5701 gtgggcgtga aaccaggacc cggccaacag caacagcaga tgggcgttgg catgccgccg
     5761 ggtatgcagc agcaacagca gcaacagcaa ccgctgcagc agcagcagat gatgcaggta
     5821 gcaatgccaa atgcgaatgc ccaaaatccg tcggcggtgg ttggcggacc caatgctcag
     5881 gtgatgggtc cgccgacgcc gcactctctg cagcagcagc tgatgcaatc ggcccgctcg
     5941 tcgccgccta ttcgctcccc gcagccaacg ccatcgccac gttcggctcc atcgccacgt
     6001 gctgctccat ccgcctcgcc tagggcacag ccctcgccgc accatgtgat gagcagtcac
     6061 tcgccagcgc cgcagggacc accgcatgac ggcatgcaca atcatggcat gcatcaccat
     6121 caccaccatt aataaggatc ctctagagtc gacctgcagg catgcaagct attcgatgca
     6181 cactcacatt cttctcctaa tacgataata aaactttcca tgaaaaatat ggaaaaatat
     6241 atgaaaattg agaaatccaa aaaactgata aacgctctac ttaattaaaa tagataaatg
     6301 ggagcggcag gaatggcgga gcatggccaa gttcctccgc caatcagtcg taaaacagaa
     6361 gtcgtggaaa gcggatagaa agaatgttcg atttgacggg caagcatgtc tgctatgtgg
     6421 cggattgcgg aggaattgca ctggagacca gcaaggttct catgaccaag aatatagcgg
     6481 tgagtgagcg ggaagctcgg tttctgtcca gatcgaactc aaaactagtc cagccagtcg
     6541 ctgtcgaaac taattaagtt aatgagtttt tcatgttagt ttcgcgctga gcaacaatta
     6601 agtttatgtt tcagttcggc ttagatttcg ctgaaggact tgccactttc aatcaatact
     6661 ttagaacaaa atcaaaactc attctaatag cttggtgttc atcttttttt ttaatgataa
     6721 gcattttgtc gtttatactt tttatatttc gatattaaac cacctatgaa gtctatttta
     6781 atcgccagat aagcaatata ttgtgtaaat atttgtattc tttatcagga aattcaggga
     6841 gacggggaag ttactatcta ctaaaagcca aacaatttct tacagtttta ctctctctac
     6901 tctagctaga gtagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6961 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     7021 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     7081 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     7141 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     7201 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7261 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     7321 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7381 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7441 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7501 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7561 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7621 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7681 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7741 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7801 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7861 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7921 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7981 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8041 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8101 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8161 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8221 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8281 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8341 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8401 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8461 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9121 cggagcctat ggaaa
//
