LOCUS       sequence-360049-        9220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Notch - NIDC/CBF1 gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE25
FEATURES             Location/Qualifiers
     source          1..9220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        58..374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      225..360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(425..427,428..430,431..1144)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(470..491)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(731..750)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1078..1099
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1216..1449
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1521..1642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1558..1577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1612..1631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1682..1817
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1837..1856)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1838..1856)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1866..1883)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1866..1882)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1875..1897)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2024..2479
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2111..2130)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2321..2342
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2505..2609
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2610..2678,2679..3470)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2828..2847)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3641..4229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4130..4149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4383..4400
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4517..4538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4553..4558,4559..4576,4577..4583)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4591..4607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4596..4618
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4615..4631
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4615..4631
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4650..4670
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4652..4670
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4698..4924
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4925..5105
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5152..5277
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5770..6003
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6188..6232
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6499..6616
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6747..6778
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      6805..6814
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6811..8460
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             8464..8583
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    join(8675..9220,1..43)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8728..8748)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9146..9157)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggcga tggtaccggt
       61 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      121 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
      181 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
      241 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
      301 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      361 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacc
      421 tagcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      481 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      541 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      601 caccctcgtg accaccttca cctacggcgt gcagtgcttc gcccgctacc ccgaccacat
      661 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      721 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      781 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
      841 gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg acaagcagaa
      901 gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca gcgtgcagct
      961 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1021 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1081 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1141 gtaaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     1201 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     1261 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     1321 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     1381 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     1441 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     1501 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     1561 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1621 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     1681 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1741 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     1801 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     1861 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     2041 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2101 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2161 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2221 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2281 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2341 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     2401 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2461 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2521 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2581 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2641 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2701 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2761 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2821 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2881 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2941 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3001 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3061 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3121 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3181 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3241 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3301 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3361 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3421 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3481 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3541 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3601 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3661 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3721 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3781 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3841 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3901 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3961 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4021 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4081 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4141 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4201 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4261 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4321 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4381 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4441 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4501 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4561 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4621 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     4681 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     4741 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     4801 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     4861 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     4921 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4981 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5041 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5101 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     5161 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     5221 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     5281 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     5341 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     5401 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     5461 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     5521 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     5581 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     5641 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     5701 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     5761 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     5821 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     5881 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     5941 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     6001 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     6061 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     6121 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     6181 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     6241 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     6301 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     6361 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     6421 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     6481 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     6541 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     6601 aaaaattcaa aattttatcg atcgcaccag cgtgtggatc cgtgggaacg tgggaacgtg
     6661 ggaacgtggg aacgtgggaa cgtgggaacg tgggaacgtg ggaagatccg agaacagatc
     6721 tggcctcggc ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct
     6781 tggcaatccg gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc
     6841 ccagcgccat tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg
     6901 aagcgctacg ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac
     6961 attacctacg ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat
     7021 gggctgaata caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg
     7081 cccgtgttgg gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac
     7141 gagcgcgagc tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag
     7201 aaagggctgc aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc
     7261 atcatggata gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc
     7321 catttgccac ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa
     7381 accatcgccc tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta
     7441 ccgcaccgca ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag
     7501 atcatccccg acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc
     7561 accacgctgg gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag
     7621 gagctattct tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca
     7681 ctatttagct tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac
     7741 gagatcgcca gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc
     7801 ttccacctac caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg
     7861 atcacccccg aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag
     7921 gctaaggtgg tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg
     7981 tgcgtccgtg gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct
     8041 ctcatcgaca aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag
     8101 cacttcttca tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc
     8161 ccagccgaac tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc
     8221 ggcctgcccg acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt
     8281 aaaaccatga ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag
     8341 aagctgcgcg gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg
     8401 gacgcccgca agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg
     8461 aattctcacg gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc
     8521 tgcgcccagg agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac
     8581 gtctaaggcc gcgactctag aacgcgtcac tgcggcttat aatgtggtca caacgactac
     8641 agacatcagc gcgtaccggt tagtaatgat cgacaatcaa cctctggatt acaaaatttg
     8701 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     8761 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     8821 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     8881 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     8941 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     9001 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     9061 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     9121 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     9181 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
//
