LOCUS       sequence-360051-        7404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An EcoRV fragment (encoding residues 1566-2142) was removed from 
            pMT-FLAG-CBP-Delta-N. Expression in S2 cells by CuSO4 induction..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Tie F, Banerjee R, Stratton CA, Prasad-Sinha J, Stepanik V, Zlobin 
            A, Diaz MO, Scacheri PC, Harte PJ
  TITLE     CBP-mediated acetylation of histone H3 lysine 27 antagonizes 
            Drosophila Polycomb silencing.
  JOURNAL   Development. 2009 Sep;136(18):3131-41. doi: 10.1242/dev.037127.
  PUBMED    19700617
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2009-09"; volume: "136"; issue: 
            "18"; pages: "3131-41"
COMMENT     Sequence Label: pMT-FLAG-CBP-Delta(N+HAT)
FEATURES             Location/Qualifiers
     source          1..7404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        411..833
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     CDS             850..873
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4381..4398
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5190..5207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5190..5206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5199..5221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5415..5434)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5534..5556
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5594..5612)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5680..5784
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5785..5853,5854..6645)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6816..7404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7305..7324
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac cgttgcagga
      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc
      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt
      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagcttt gcatcccata
      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac
      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag
      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc
      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggaattc
      841 gagctcatgg actacaagga cgacgatgac aaggctagca atccactgct cctcatgtct
      901 ggaggaacgg caggaggcgg aacgggagca acgaccacca catcgacatc ctcgagcagt
      961 cgcatgatga gcagctccag cagtctctcc tcacagatgg ctgccctgga ggctgcggcg
     1021 cgagacaacg acgatgagac gccctcgcca tccggcgaga atacgaacgg cagtggtggc
     1081 agtggaaatg ccggcggtat ggcctccaag ggcaaactgg actccattaa gcaagatgat
     1141 gatatcctga agcaggcgat ggaggacaaa ctgggctctg ccgcagagct gccctacttt
     1201 gagggcgact tctggcccaa tgtgctggag gagagcatca aggaactgga ccaggaggag
     1261 gaagagaagc gcaaacaggc cgaggccgcg gaagcagcag ctgcggcaaa tcttttctct
     1321 atcgaggaaa atgaagtaag cggcgatggc aaaaagaagg gccagaagaa ggccaaaaag
     1381 tcgaacaaat cgaaagcggc gcagcgtaag aacagcaaaa agtccaacga acatcagtcg
     1441 ggcaatgatc tctccacaaa gatatatgcg accatggaga agcacaagga ggtcttcttc
     1501 gttatccgtc tgcattcggc gcagtcggca gctagtttag cgcccatcca ggatcccgat
     1561 ccgctgctca catgcgatct gatggatgga cgcgatgcct tcctcacgct cgcccgcgac
     1621 aagcactttg agttctcgtc gctgcggcgc gcacaattct ccactctgtc catgttgtat
     1681 gagctgcata accagggtca ggacaagttt gtttacacct gcaaccactg caagacggcc
     1741 gtggagacgc gctaccactg tactgtttgt gatgacttcg atctgtgtat cgtgtgcaag
     1801 gagaaggttg gccatcagca caagatggag aagctcggct tcgacatcga cgacggctct
     1861 gcgctggcgg atcacaagca ggctaatcca caggaggccc gcaagcaatc catccagcgt
     1921 tgcatccaat cgctggcgca cgcctgccag tgtcgcgatg ccaactgccg cctgccatcg
     1981 tgccagaaga tgaagctcgt tgtccagcat acgaagaact gcaagcgcaa gcccaacgga
     2041 ggatgcccca tttgcaagca gcttatcgca ctctgttgct atcacgcgaa gaactgtgag
     2101 gagcagaagt gccccgtgcc gttctgtccc aacatcaagc acaagctcaa gcagcagcag
     2161 tcacagcaga aattccagca gcagcagttg ctgcgtcgcc gtgtggcgct catgtcgcgt
     2221 acagcagctc cagcggctct gcaaggccca gctgcagtaa gcggtccgac cgtcgtctct
     2281 ggaggagtgc ccgtggtggg catgtccggt gtggcagtta gccaacaggt gatccccggc
     2341 caggcgggta tactgcctcc aggggcgggt ggcatgtcgc catctaccgt ggcagttcca
     2401 tcgcctgttt caggaggagc gggagccggt ggaatgggtg gaatgacatc accacatccg
     2461 catcaaccag gtataggtat gaaacctggt ggcggtcact cgccgtctcc aaatgtccta
     2521 caagtggtga agcaggtcca ggaagaggca gctcgtcagc aggtatcgca tggcggtggc
     2581 ttcggcaagg gcgtacccat ggcgccgccc gtaatgaatc gaccaatggg cggcgctggg
     2641 cccaaccaaa atgttgttaa tcaacttggt ggcatgggcg ttggagttgg aggtgtcggt
     2701 ggtgttggcg tcggaggcgt tggtggagtg ggtgttaatc aactgaattc gggtggtggc
     2761 aatacacccg gtgcacccat ttccggtccc ggaatgaatg tcaatcatct aatgtccatg
     2821 gatcagtggg gcggtggcgg agccggcggc ggaggtgcca atcccggcgg tggcaatcca
     2881 caagcccgct atgccaacaa taccggcggc atgcgccaac ccacccatgt gatgcaaacg
     2941 aatctgatac cgccgcagca acagcaacag atgatgggcg gactgggcgg acccaaccaa
     3001 ctgggaggtg gccaaatgcc agtcggcgga cagcatggag gaatgggaat gggcatggga
     3061 gcaccaccaa tggccggaac tgttggcgga gtgcgtccat ctcccggagc aggaggtgga
     3121 ggtggaagtg cgactggggg cggtctaaat acgcaacaac tcgccctgat tatgcaaaag
     3181 attaagaaca atcccaccaa cgagagcaac cagcacatcc ttgccatact aaaacagaat
     3241 ccgcagatca tggcggcgat catcaagcag cgccagcagt cgcagaacaa tgcggcagcg
     3301 ggcggaggag cacctggccc aggtggagcc ctacagcagc agcaggccgg taacggaccg
     3361 caaaatcctc aacagcagca gcagcagcag caacagcaac aggtgatgca gcaacagcag
     3421 atgcagcaca tgatgaacca gcagcagggc ggcggcggtc cacagcagat gaatcccaac
     3481 cagcagcagc aacagcagca ggttaatctc atgcagcagc agcaacaagg tggacccgga
     3541 ggaccaggtt ctggacttcc cacgcgcatg cccaatatgc ccaatgcctt gggtatgctg
     3601 cagagtcttc cgcccaacat gtcgccaggc gtttctactc agggaggaat ggtgcccaac
     3661 caaaactgga acaagatgcg ttacatgcaa atgagccagt acccgccacc gtatccgcag
     3721 cgccagcgtg gcccgcacat gggcggagcg ggacctggtc ccggccagca acagttcccc
     3781 ggtggcggag gtggagcggg caactttaat gcgggtggtg ctggtggtgc aggcggcgtt
     3841 gtcggtgtgg gcggagtgcc cggaggtgcc ggcacggtgc ccggtggcga tcaatactcg
     3901 atggcgaatg ccgcggctgc ctccaatatg ctgcaacagc agcagggcca ggtgggcgtc
     3961 ggagtgggcg tgggcgtgaa accaggaccc ggccaacagc aacagcagat gggcgttggc
     4021 atgccgccgg gtatgcagca gcaacagcag caacagcaac cgctgcagca gcagcagatg
     4081 atgcaggtag caatgccaaa tgcgaatgcc caaaatccgt cggcggtggt tggcggaccc
     4141 aatgctcagg tgatgggtcc gccgacgccg cactctctgc agcagcagct gatgcaatcg
     4201 gcccgctcgt cgccgcctat tcgctccccg cagccaacgc catcgccacg ttcggctcca
     4261 tcgccacgtg ctgctccatc cgcctcgcct agggcacagc cctcgccgca ccatgtgatg
     4321 agcagtcact cgccagcgcc gcagggacca ccgcatgacg gcatgcacaa tcatggcatg
     4381 catcaccatc accaccatta ataaggatcc tctagagtcg acctgcaggc atgcaagcta
     4441 ttcgatgcac actcacattc ttctcctaat acgataataa aactttccat gaaaaatatg
     4501 gaaaaatata tgaaaattga gaaatccaaa aaactgataa acgctctact taattaaaat
     4561 agataaatgg gagcggcagg aatggcggag catggccaag ttcctccgcc aatcagtcgt
     4621 aaaacagaag tcgtggaaag cggatagaaa gaatgttcga tttgacgggc aagcatgtct
     4681 gctatgtggc ggattgcgga ggaattgcac tggagaccag caaggttctc atgaccaaga
     4741 atatagcggt gagtgagcgg gaagctcggt ttctgtccag atcgaactca aaactagtcc
     4801 agccagtcgc tgtcgaaact aattaagtta atgagttttt catgttagtt tcgcgctgag
     4861 caacaattaa gtttatgttt cagttcggct tagatttcgc tgaaggactt gccactttca
     4921 atcaatactt tagaacaaaa tcaaaactca ttctaatagc ttggtgttca tctttttttt
     4981 taatgataag cattttgtcg tttatacttt ttatatttcg atattaaacc acctatgaag
     5041 tctattttaa tcgccagata agcaatatat tgtgtaaata tttgtattct ttatcaggaa
     5101 attcagggag acggggaagt tactatctac taaaagccaa acaatttctt acagttttac
     5161 tctctctact ctagctagag tagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     5221 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5281 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5341 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     5401 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5461 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5521 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5581 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5641 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5701 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5761 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5821 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5881 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5941 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6001 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6061 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6121 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6181 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6241 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6301 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6361 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6421 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6481 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6541 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6601 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6661 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6721 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6781 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6841 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6901 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6961 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7021 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7081 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7141 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7201 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7261 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7321 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7381 tcaggggggc ggagcctatg gaaa
//
