LOCUS       sequence-360052-        2253 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 
            2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I 
            sites. Expression in E. coli. by IPTG induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Tie F, Banerjee R, Saiakhova AR, Howard B, Monteith KE, Scacheri PC,
            Cosgrove MS, Harte PJ
  TITLE     Trithorax monomethylates histone H3K4 and interacts directly with 
            CBP to promote H3K27 acetylation and antagonize Polycomb silencing.
  JOURNAL   Development. 2014 Mar;141(5):1129-39. doi: 10.1242/dev.102392.
  PUBMED    24550119
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-03"; volume: "141"; issue: 
            "5"; pages: "1129-39"
COMMENT     Sequence Label: pGEX-2T-TRX-C751-E3616S
FEATURES             Location/Qualifiers
     source          1..2253
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatccata ttccccagca gcaacaacct ctgcagcaac aacaggtgca ggtacagccg
       61 tcaatgccca ttataacact tgctgaggca ccagtagtgc aatcgcaatt tgtgatggaa
      121 cctcaagctt tggagcagca ggagctggct aaccgcgtgc agcatttctc cacaagcagc
      181 agcagtagca gtagcaactg ctcactgccc accaatgtgg ttaatcctat gcaacaacaa
      241 gcaccatcaa ccaccagcag ctccacaacc aggcctacaa atcgggtgct accgatgcag
      301 cagcgccagg aacctgctcc gttgtccaac gagtgtcccg tagtttcgtc acccactcct
      361 ccgaaacctg ttgaacagcc tataatccat caaatgacta gcgccagtgt gtccaagtgc
      421 tatgcccaaa agtcaacact accatcacca gtttacgagg cggaactcaa agttagttca
      481 gttttggaga gcattgtgcc agatgtcaca atggatgcaa tcttggagga acagccggta
      541 acggaatcaa tctatacgga gggactctac gagaagaatt cgccggggga gtcgaagact
      601 gaacagctac ttctccaaca acagcagcga gagcaactta atcaacaatt ggtcaacaac
      661 gggtatctgc tcgataagca cacctttcaa gtggagccaa tggatacgga tgtctacagg
      721 gaggaagacc tcgaagagga ggaggacgag gatgatgatt tcagcttgaa aatggccaca
      781 tcggcgtgca acgatcacga gatgtccgac agtgaagaac cggcggtcaa ggataagatc
      841 agtaagattc tggacaacct aaccaacgat gactgtgcag actcaatagc cacggcgacg
      901 acgatggaag tggatgccag cgctggttac cagcaaatgg tggaggatgt cctagccacc
      961 actgcagcac aatctgctcc caccgaagag ttcgaaggcg ctctagaaac tgcagctgtg
     1021 gaagccgctg ccacctatat caacgagatg gccgatgctc atgtactgga ccttaagcag
     1081 ctgcaaaatg gagtggaact agagctcagg aggagaaaag aagagcaacg aacggtctcg
     1141 caagaacagg agcaatcaaa ggccgccatc gtgcctacag cagctgctcc cgaaccaccg
     1201 caacccattc aagagcccaa aaaaatgact ggtccacatc tgctttatga aatacaaagc
     1261 gaagatggct ttacgtacaa atcgagttcc ataactgaga tttgggagaa ggtctttgag
     1321 gcagtacagg tggccaggcg ggcacatgga cttactccgc tgccagaggg tcctctagcc
     1381 gatatgggtg gcatccagat gataggtctt aaaacgaatg cccttaagta cctaattgaa
     1441 cagttgccag gcgtggaaaa gtgctcaaaa tatactccca agtatcacaa gcgcaatggt
     1501 aacgtgtcca cagcggcaaa tggagcacat ggaggcaact taggcggaag cagtgcatcc
     1561 gctgccctct cagtgtctgg tggagattca catggtctgc tcgactatgg atcggatcag
     1621 gatgaattgg aggaaaatgc ttacgattgc gcccgatgtg agccttattc caaccgcagc
     1681 gagtatgata tgtttagttg gttggcctcc agacatcgca agcagcccat ccaggtgttc
     1741 gtccagccgt ccgacaatga actggtaccc aggaggggaa ctggtagcaa tctacccatg
     1801 gccatgaagt atcggactct taaggaaact tacaaggatt atgttggagt cttcagatcc
     1861 catatccatg gacgaggcct ctactgcact aaggacattg aagcgggtga aatggttatc
     1921 tcatatgccg gtgagttgat tcgctctaca ctgacggaca aaagggaacg ctactacgac
     1981 agccgcggca ttgggtgcta tatgttcaaa attgacgata accttgtagt tgatgccacg
     2041 atgcgcggca atgcggcgcg tttcattaat cactgctgtg agccgaattg ctattcgaaa
     2101 gtggtggaca tcttagggca caagcacatc atcatctttg cgctgcgccg aatcgtgcag
     2161 ggcgaggagc ttacctacga ctacaaattc ccattcgagg atgagaagat accttgctca
     2221 tgcggttcga aaaggtgtcg gaagtactta aat
//
