LOCUS       sequence-360057-        8181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) 
            was used for in vitro acetylation assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Tie F, Banerjee R, Conrad PA, Scacheri PC, Harte PJ
  TITLE     Histone demethylase UTX and chromatin remodeler BRM bind directly to
            CBP and modulate acetylation of histone H3 lysine 27.
  JOURNAL   Mol Cell Biol. 2012 Jun;32(12):2323-34. doi: 10.1128/MCB.06392-11. 
            Epub 2012 Apr 9.
  PUBMED    22493065
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.06392-11"; journalName: "Mol Cell Biol"; date: 
            "2012-06"; volume: "32"; issue: "12"; pages: "2323-34"
COMMENT     Sequence Label: pGEX-2T-CBP(1603-2678)
FEATURES             Location/Qualifiers
     source          1..8181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(183..188,189..204,205..211)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     869..891
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             918..935
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(4231..4253)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4398..4416)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4484..4588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4589..4657,4658..5449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4807..4826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5620..6208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6109..6128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6362..6379
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        6452..6529
                     /gene="lacI"
                     /label=lacI promoter
     CDS             6530..7612
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6549..6568)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(7661..7666,7667..7684,7685..7691)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7699..7715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7723..7739
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7723..7739
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(7735..8181,1..81)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(7755..7772)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7755..7771)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7764..7786)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gttccgcgtg gatcccatat gatcaaaatg gagatcaaga
      961 cggaggatgg actcgatggc gaggtaaaga tcaaaacgga ggccatggat gtggacgagg
     1021 ctggaggatc gacagccgga gagcatcatg gcgaaggtgg cggcggcagt ggtgttggcg
     1081 gcggtaagga taacataaat ggtgcgcacg atggcggagc gacaggcggt gctgtggata
     1141 taaaacccaa gacggagacg aaaccactcg taccggagcc actggcaccc aatgcaggtg
     1201 acaagaaaaa gaagtgccaa ttcaatcccg aggaactgcg caccgctctc ctgccaacgc
     1261 tagagaagct ctacaggcag gagcccgaat ccgtgccctt tcgctaccca gttgatcccc
     1321 aggcgctggg catacctgat tactttgaaa tcgtaaagaa gcccatggac ctgggcacta
     1381 tacgcaccaa catccagaat ggaaagtaca gtgatccctg ggaatatgtg gacgacgttt
     1441 ggctgatgtt cgacaatgcc tggctgtata atcgcaaaac atcgcgggtc tatcgctatt
     1501 gcacaaagct ttccgaagtc tttgaggcgg agattgatcc tgtgatgcag gcactgggat
     1561 attgctgcgg caggaagtac acattcaatc cacaggtgct atgctgctac ggcaagcagc
     1621 tctgcacgat tccgcgggat gccaagtact acagctacca gaacagtcta aaggaatacg
     1681 gtgtcgcctc aaatagatac acctactgcc aaaagtgctt taacgacatc cagggcgata
     1741 cggtcacact gggcgacgat ccactgcaat cgcaaaccca aatcaaaaag gatcagttca
     1801 aggagatgaa gaacgatcac ctcgaactgg agccgtttgt caattgccag gagtgcggac
     1861 gcaaacagca ccaaatctgc gtactctggc tggattctat ctggcccggt ggcttcgtgt
     1921 gcgataactg cctgaaaaag aagaactcaa agcggaagga gaacaagttc aatgcgaaac
     1981 gcctgcccac caccaagctg ggcgtgtaca tagagacgcg ggtgaataat ttcctcaaga
     2041 agaaggaggc tggtgccggc gaggtgcaca ttcgtgtggt cagctcatcg gacaagtgtg
     2101 tagaggtgaa gcccggcatg cgtcgacgat tcgttgagca gggcgagatg atgaacgagt
     2161 tcccataccg agccaaagcg ctctttgcct tcgaggaggt ggatggcatc gatgtgtgct
     2221 tctttggcat gcacgttcag gagtatggat ccgagtgccc ggcgccgaat acgcggcgtg
     2281 tgtatattgc ctatttggat tccgttcatt tcttccggcc aagacagtac cgtacagcgg
     2341 tatatcacga aatcctgctc ggctatatgg actacgtgaa acagctgggc tacacaatgg
     2401 cccatatctg ggcctgtccg ccatccgagg gcgatgacta catctttcac tgccatccca
     2461 cggaccagaa gatacccaag cccaagcgcc tgcaggagtg gtacaaaaag atgcttgaca
     2521 agggaatgat cgagcgcatc atacaggact acaaggatat cctgaagcag gcgatggagg
     2581 acaaactggg ctctgccgca gagctgccct actttgaggg cgacttctgg cccaatgtgc
     2641 tggaggagag catcaaggaa ctggaccagg aggaggaaga gaagcgcaaa caggccgagg
     2701 ccgcggaagc agcagctgcg gcaaatcttt tctctatcga ggaaaatgaa gtaagcggcg
     2761 atggcaaaaa gaagggccag aagaaggcca aaaagtcgaa caaatcgaaa gcggcgcagc
     2821 gtaagaacag caaaaagtcc aacgaacatc agtcgggcaa tgatctctcc acaaagatat
     2881 atgcgaccat ggagaagcac aaggaggtct tcttcgttat ccgtctgcat tcggcgcagt
     2941 cggcagctag tttagcgccc atccaggatc ccgatccgct gctcacatgc gatctgatgg
     3001 atggacgcga tgccttcctc acgctcgccc gcgacaagca ctttgagttc tcgtcgctgc
     3061 ggcgcgcaca attctccact ctgtccatgt tgtatgagct gcataaccag ggtcaggaca
     3121 agtttgttta cacctgcaac cactgcaaga cggccgtgga gacgcgctac cactgtactg
     3181 tttgtgatga cttcgatctg tgtatcgtgt gcaaggagaa ggttggccat cagcacaaga
     3241 tggagaagct cggcttcgac atcgacgacg gctctgcgct ggcggatcac aagcaggcta
     3301 atccacagga ggcccgcaag caatccatcc agcgttgcat ccaatcgctg gcgcacgcct
     3361 gccagtgtcg cgatgccaac tgccgcctgc catcgtgcca gaagatgaag ctcgttgtcc
     3421 agcatacgaa gaactgcaag cgcaagccca acggaggatg ccccatttgc aagcagctta
     3481 tcgcactctg ttgctatcac gcgaagaact gtgaggagca gaagtgcccc gtgccgttct
     3541 gtcccaacat caagcacaag ctcaagcagc agcagtcaca gcagaaattc cagcagcagc
     3601 agttgctgcg tcgccgtgtg gcgctcatgt cgcgtacagc agctccagcg gctctgcaag
     3661 gcccagctgc agtaagcggt ccgaccgtcg tctctggagg agtgcccgtg gtgggcatgt
     3721 ccggtgtggc agttagccaa caggtgatcc ccggccaggc gggtatactg cctccagggg
     3781 cgggtggcat gtcgccatct accgtggcag ttccatcgcc tgtttcagga ggagcgggag
     3841 ccggtggaat gggtggaatg acatcaccac atccgcatca accaggtata ggtatgaaac
     3901 ctggtggcgg tcactcgccg tctccaaatg tcctacaagt ggtgaagcag gtccaggaag
     3961 aggcagctcg tcagcaggta tcgcatggcg gtggcttcgg caagggcgta cccatggcgc
     4021 cgcccgtaat gaatcgacca atgggcggcg ctgggcccaa ccaaaatgtt gttaatcaac
     4081 ttggtggcat gggcgttgga gttggaggtg tcggtggtgt tggcgtcgga ggcgttggtg
     4141 gagtgggtgt taatcaactg aattcgatgc atgaattcat cgtgactgac tgacgatctg
     4201 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     4261 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     4321 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa
     4381 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4441 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4501 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4561 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4621 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4681 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4741 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4801 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     4861 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4921 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4981 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5041 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5101 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     5161 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5221 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5281 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5341 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     5401 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5461 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5641 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5701 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5761 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5821 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5881 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5941 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6001 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6061 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6121 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6181 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6241 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6301 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6361 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     6421 tctgtgcggt atttcacacc gcataaattc cgacaccatc gaatggcgca aaacctttcg
     6481 cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt
     6541 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt
     6601 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
     6661 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
     6721 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa
     6781 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt
     6841 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat
     6901 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc
     6961 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga
     7021 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct
     7081 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata
     7141 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc
     7201 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt
     7261 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt
     7321 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc
     7381 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     7441 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     7501 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7561 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7621 caattaatgt aagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7681 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7741 atgattacgg attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7801 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7861 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc
     7921 tggtttccgg caccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc
     7981 gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc
     8041 aacgtgacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt
     8101 tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt
     8161 atttttgatg gcgttggaat t
//
