LOCUS       sequence-360062-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. 
            pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in 
            our paper (Tie et al. 2012 MCB, Tie et al 2014 Development).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Tie F, Banerjee R, Saiakhova AR, Howard B, Monteith KE, Scacheri PC,
            Cosgrove MS, Harte PJ
  TITLE     Trithorax monomethylates histone H3K4 and interacts directly with 
            CBP to promote H3K27 acetylation and antagonize Polycomb silencing.
  JOURNAL   Development. 2014 Mar;141(5):1129-39. doi: 10.1242/dev.102392.
  PUBMED    24550119
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-03"; volume: "141"; issue: 
            "5"; pages: "1129-39"
COMMENT     Sequence Label: pGEX-2T-CBP(1-287)
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgatggccg atcacttaga cgaaccgccc caaaagcggg ttaaaatgga tccaacggat
       61 atctcttact ttctggagga gaacctgccc gatgagctgg tgtcctcgaa tagtggctgg
      121 tcggatcagc tgaccggcgg agcaggcggt ggcaatggag gtggcggcgc ctccggtgta
      181 accacaaatc ccacatccgg cccaaatccc ggtggcggac ccaacaagcc ggcagcccaa
      241 ggacccggct ctggcacagg cggagtcggt gttggagtga atgtgggtgt cggcggtgtt
      301 gttggcgtcg gcgttgtgcc ttcccagatg aacggagccg gcggcggcaa cggatccgga
      361 acgggtggcg acgacggcag tggcaacggc tcaggagcgg gcaacagaat cagtcaaatg
      421 caacaccagc aactgcagca cctactccag cagcagcagc agggccagaa gggcgccatg
      481 gtggtgcccg gcatgcagca gctgggcagc aagtcgccca acctgcagtc acccaaccag
      541 ggcggcatgc agcaggtggt gggcactcag atgggtatgg tcaactcaat gcccatgtca
      601 atatcgaata atggcaacaa tggcatgaac gccataccag gcatgaacac cattgcgcag
      661 ggcaatctgg gaaacatggt gctgaccaac agcgttggcg gcggcatggg cggcatggtt
      721 aatcatctta agcagcagcc tggcggcggc ggcggtggga tgatcaattc cgtttcagta
      781 cccggaggac ctggagcagg agctggtggc gttggagctg gcggcggagg agccgttgcc
      841 gcaaaccaag gcatgcat
//
