LOCUS       sequence-36007-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Sanders TA, Lumsden A, Ragsdale CW
  TITLE     Arcuate plan of chick midbrain development.
  JOURNAL   J Neurosci. 2002 Dec 15. 22(24):10742-50.
  PUBMED    12486167
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2002 Dec 15. 22(24):10742-50."
COMMENT     Sequence Label: pcTUBB3
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(477..493)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(478..494)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(518..537)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(519..537)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(547..564)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(547..563)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(792..811)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtggcggc cgaggagggc gagatgtacg aggatgatga ggaggagtcg gaggcgcagg
       61 gcgccaagtg acggccccac actcgctgct tcgggggggc ctctggcctc acccctcggt
      121 gtctgctccc ctcagcccca tgttagctca accccccccc gtctccctcc ctatggcctc
      181 cccttcccct tttttcgttt tttactgttt gtgtctcata ttttttttga atactcaata
      241 aatttatggc tgtccggtta ctcctggctc tggttgctgg catggggtgg ggggggctca
      301 tatagggggt agtgggaggg gggcataata ttccctttta aacccctaat ggaagaaaaa
      361 ttggggacca cgagagcccc ctggggccat gcccaaattc tggtcagtaa cactgagtgc
      421 caccccatgt actgctgcaa agcccccggg ctgcaggaat tcgatatcaa gcttatcgat
      481 accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc
      541 gcgctcactg gccgtcgttt tacaacgnnn tgactgggaa aaccctggcg ttacccaact
      601 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      661 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
      721 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      781 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      841 ccgtcaagct ctaaatcggg ggctcccttt a
//
