LOCUS       sequence-360071-        1170 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express PC-H6 in E. coli. Purified PC-H6 was used for for generating
            antibodies in rabbits and for GST-CBP pull-down assays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Tie F, Banerjee R, Fu C, Stratton CA, Fang M, Harte PJ
  TITLE     Polycomb inhibits histone acetylation by CBP by binding directly to 
            its catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 9;113(6):E744-53. doi: 
            10.1073/pnas.1515465113. Epub 2016 Jan 22.
  PUBMED    26802126
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1515465113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-9- 9"; volume: "113"; issue: "6"; pages: "E744-53"
COMMENT     Sequence Label: pET11-PC-H6
FEATURES             Location/Qualifiers
     source          1..1170
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             400..429
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             478..501
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 atgactggtc gaggcaaggg gagtaagggg aagttggggc gcgacaatgc gaccgacgat
       61 ccagtcgatc tagtgtacgc ggctgagaaa atcatccaaa agcgcgttaa gaagggcgtc
      121 gtggagtacc gtgtcaagtg gaagggctgg aaccagcgct acaacacctg ggaaccggag
      181 gtaaacatcc tggatcgccg cctcatcgac atctacgaac aaacgaacaa atcctccgga
      241 actccctcca agcgaggcat taagaagaag gagaaagaac ccgatccgga gccggaatcc
      301 gaggaggatg agtacacctt caccgaaaac gatgtggaca cgcatcaagc caccacctca
      361 tcggctaccc acgataagga gtcgaagaag gagaagaagc accatcacca ccaccaccat
      421 catcaccaca tcaagtccga acgcaacagt ggacggcgct cggaatctcc gctgacccac
      481 catcatcatc accaccacca cgagtccaag cgtcagcgca ttgatcacag ctcctcctcg
      541 aacagcagct tcacgcacaa ctcatttgtc cccgagccgg acagcaactc ctccagctcc
      601 gaggatcagc cgctgatcgg caccaaacgc aaagccgagg tgctcaagga atcgggcaag
      661 attggagtta cgattaagac ctccccagat ggccccacta ttaagcctca gccgacccag
      721 caggtaactc ccagccagca gcagcccttc caggatcagc agcaagcgga aaagattgcc
      781 agcgaggctg caacgcagct gaaatctgag cagcaggcca ccccactggc cacagaagcc
      841 ataaacacaa cgcccgcaga gtccggagct gaggaagaag aagtagccaa cgaggaaggc
      901 aaccaacagg cgccacaggt tccttccgag aacaataaca taccaaaacc gtgcaacaac
      961 ctggctatca accagaaaca gccgcttact cctctttcgc cgcgtgccct tccgccgcgc
     1021 ttctggctgc cggccaaatg caacatatcc aaccgggtgg tgatcacaga cgtcaccgta
     1081 aacctggaga ccgttaccat acgcgagtgc aagacggagc gcgggttttt tcgtgagcgc
     1141 gacatgaaag gcgattcgtc gccagtagct
//
