LOCUS       sequence-360084-        9394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Koopal B, Potocnik A, Mutte SK, Aparicio-Maldonado C, Lindhoud S, 
            Vervoort JJM, Brouns SJJ, Swarts DC
  TITLE     Short prokaryotic Argonaute systems trigger cell death upon 
            detection of invading DNA.
  JOURNAL   Cell. 2022 Apr 28;185(9):1471-1486.e19. doi: 
            10.1016/j.cell.2022.03.012. Epub 2022 Apr 4.
  PUBMED    35381200
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.012"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1471-1486.e19"
COMMENT     Sequence Label: pBK086
FEATURES             Location/Qualifiers
     source          1..9394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(278..1360)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1322..1341
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1361..1438)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1644..1663
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1747..1766
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1747..1765
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1766..1790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1805..1827
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1846..1863
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1873..2973
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2947..2970
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2986..3006
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             6004..6021
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6074..6092)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6088..6135
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6172..6627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6259..6278)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6469..6490
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6720..7535)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6720..7535)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7443..7462
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7527..7546
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7657..8245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8146..8165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8399..8416
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8431..8570
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8496..8515)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8656..8678
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8672..8863)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
       61 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
      121 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
      181 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
      241 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      301 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      361 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
      421 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
      481 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
      541 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
      601 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg ataccctcat
      661 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
      721 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
      781 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
      841 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
      901 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
      961 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     1021 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     1081 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     1141 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     1201 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     1261 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     1321 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     1381 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     1441 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     1501 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     1561 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     1621 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     1681 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
     1741 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
     1801 taattttgtt taactttaag aaggagatat accatgggtt cttctcacca tcaccatcac
     1861 catggttctt ctatgaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa
     1921 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
     1981 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
     2041 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
     2101 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
     2161 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg
     2221 atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg
     2281 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac
     2341 ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag
     2401 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg
     2461 gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
     2521 gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc
     2581 gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc
     2641 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
     2701 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa
     2761 gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca
     2821 cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag
     2881 atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag
     2941 actgtcgatg aagccctgaa agacgcgcag actaatggga tcgaggaaaa cctgtacttc
     3001 caatccaatg caagaaataa aatatttata agtcacgcaa cacccgatga caatgatttt
     3061 actagatggt tagcgttaaa actaattgga ttaggatacg aagtttggtg tgacattctt
     3121 tttttagata aaggggttga tttctggagc aatatcgaaa aagttataag agaagatacc
     3181 tgtaaatttt tacttgtttc atcatcttat tcaaatcaac gcgaaggtgt tcttaaagaa
     3241 ttagcagttg cagcaaaagt aaaaaagcaa ctgaaagatg ataagttcat tattccatta
     3301 gcaattgatg aacaactatc ttacgatgat attaatattg atattgttag gcttaacgct
     3361 atcgatttta aaatgtcttg ggcaagaggt ttgaaagata tattagaagc gttcgaaaaa
     3421 caaaaagttc caaaagaagt tgctgacgct tcaaaaagta atttacttta tcaacaaatt
     3481 tttctgcacg ataaaagtgt aattgaaaaa gaagaaattt atgactcaaa ttggctttcg
     3541 atattgtcct ttcctgaaga gttaagattt cacgaataca attggatgct tcctaaaaga
     3601 tttgacgtaa gagaactgac gtttcctgct gttcgttaca aaaattatct ctgtacgttc
     3661 gcttgggcct acgactttac atatcattta cctaaaacag aaacatatca taagagcaaa
     3721 acaattagga taccaacaga ggaaatttta tcaggaagtt atgattctaa ttttattaga
     3781 aatgcagaat gtaaaaggct aatagtacaa cttcttaata aagcatttga attaaggatg
     3841 aaagataaag aagttcaaga atatgaaatg tctaataaaa ccgcatattg gctcgaaaaa
     3901 ggtaagttag aaaaagacaa atttgagaaa accatgttag tcggaaagca aaaagataaa
     3961 aattggcatt tcgcaatttc tggagcttca aaactttatc cctttcctgt attaatgatt
     4021 tcatcacaca tattttttac ggcagacgga aaaaaattga ttgactcttc aagcgttcaa
     4081 cattcatcaa gaagaagaca aggtaaaaac tggtggaata atacttggag aaccaaacta
     4141 ttggcattta ttaaatattt atccgacgat gacacttcct tttatttaga aatgggtagt
     4201 gaagagaaag tttttgtatc caatgaacca gtaaaattta aaggtaacgt aagctataat
     4261 attcctgaaa aaaatactct tgaagaagaa gctgaattat cgggctttaa ccaaggcgaa
     4321 gatatcgagg agctggaaga gttgattgaa aatttagaag ctgaataact cgaagcttag
     4381 attttaaata acaatactaa ggaggaatta catatgaaag aacttatata catcgaagaa
     4441 ccaaaaatac tatttgcaca cggtcaaaaa tgtactgacg ccagagatgg acttgcctta
     4501 tttggtccat taaataatct ttatggcatt aagagtggcg ttattggtac taaacaagga
     4561 ctgaaaatat ttagagatta tttagaccac atacaaaaac caatctataa tagtaacagt
     4621 attactagac cgatgtttcc cggttttgaa gctgttttcg attgtaaatg ggaatccaca
     4681 ggaatcacat ttaaagaagt tacaaatgaa gatattggta aatttcttta caatagcagt
     4741 acgcataagc gaacttacga cttagtttcc ctttttatag acaagatcat ttctgctaat
     4801 aaaaatgaag atgaaaatgt cgatgtatgg ttcgtcattg ttccagatga aatttataaa
     4861 tattgcagac caaattcagt acttcccaaa gaaatggttc agactaaggc actaatgtct
     4921 aaatcaaaag caaaatcttt caggtatgaa ccatcactat tcccagatat caacatagaa
     4981 ttaaaagaac aagaaaaaga ggctgaaact tataattatg acgcacagtt tcacgaccaa
     5041 tttaaggcaa gattattaaa acacaccata ccaacacaaa tttttagaga aagtacgttg
     5101 gcttggcgcg atttcaaaaa tgcatttggt ctgccaatta gagatttttc taaaattgaa
     5161 gggcatttgg cttggacaat ttcaacagct gcgttttaca aagctggagg taaaccttgg
     5221 aaattatctg atgtccgaaa tggagtatgc tatcttggtt tagtttataa aaaagttgaa
     5281 aaaagtaaaa acccaagaaa tgcttgttgt gcagctcaaa tgtttttaga taatggagat
     5341 ggtacggttt ttaaaggaga agttggacct tggtacaatc ctaaaaatgg acagtatcat
     5401 ttagaaccta aagaagcgaa ggctctgtta agtcaatcct tacaatccta taaagaacag
     5461 attggagaat atcctaaaga ggtattcatt cacgcaaaaa caaggtttaa tcatcaagaa
     5521 tgggatgcat ttttagaagt gacaccaaaa gaaactaatc ttgtcggagt aaccatttca
     5581 aaaacaaaac cactcaaatt atacaaaact gagggagatt atacaatttt aagaggaaat
     5641 gcatatgtag ttaacgagag aagtgccttt ctatggactg tgggctatgt tcctaaaata
     5701 caaactgcat tatcaatgga ggttcctaat cctttattta tagaaataaa taaaggagaa
     5761 gcagatataa aacaagtttt aaaggatatt ttatctttaa ctaaattgaa ttataatgcc
     5821 tgcatttttg cagatggtga acctgtaacg ttaagatttg cggacaagat tggagaaata
     5881 cttactgcga gtactgacat aaaaacaccg cctttagcat ttaaatatta tatctaataa
     5941 cattggaagt ggataacgga tccgaattcg agcgccgtcg acaagcttgc ggccgcactc
     6001 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
     6061 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     6121 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
     6181 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6241 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6301 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6361 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6421 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6481 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6541 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6601 ttaacaaact agtaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa
     6661 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct
     6721 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     6781 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     6841 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     6901 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     6961 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     7021 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     7081 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     7141 tgcaaccggc gcaggaacac tgccagcgca tcaacaatgt tttcacctga atcaggatat
     7201 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     7261 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     7321 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     7381 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     7441 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     7501 ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     7561 taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     7621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     7681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7801 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7921 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7981 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8041 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8101 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8161 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8221 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8281 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8341 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8401 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     8461 gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8521 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     8581 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     8641 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     8701 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     8761 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     8821 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     8881 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     8941 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     9001 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     9061 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     9121 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     9181 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     9241 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     9301 acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct
     9361 tctcgccgaa acgtttggtg gcgggaccag tgac
//
