LOCUS       sequence-360089-        9388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Koopal B, Potocnik A, Mutte SK, Aparicio-Maldonado C, Lindhoud S, 
            Vervoort JJM, Brouns SJJ, Swarts DC
  TITLE     Short prokaryotic Argonaute systems trigger cell death upon 
            detection of invading DNA.
  JOURNAL   Cell. 2022 Apr 28;185(9):1471-1486.e19. doi: 
            10.1016/j.cell.2022.03.012. Epub 2022 Apr 4.
  PUBMED    35381200
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.03.012"; journalName: "Cell"; date: 
            "2022-04-28- 28"; volume: "185"; issue: "9"; pages: "1471-1486.e19"
COMMENT     Sequence Label: pBK092
FEATURES             Location/Qualifiers
     source          1..9388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(319..1401)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1363..1382
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1402..1479)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1685..1704
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1788..1807
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1788..1806
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1807..1831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1846..1868
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1887..1904
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1914..3014
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2988..3011
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             3027..3047
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             6039..6056
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6109..6127)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6123..6170
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6207..6662
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6294..6313)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6504..6525
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6755..7570)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6755..7570)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7478..7497
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7562..7581
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7692..8280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8181..8200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8434..8451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8466..8605
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8531..8550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8691..8713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8707..8898)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg
       61 tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg
      121 tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag
      181 aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact
      241 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
      301 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
      421 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
      481 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
      541 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
      601 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
      661 atcgttggca accagcatcg cagtgggaac gataccctca ttcagcattt gcatggtttg
      721 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
      781 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
      841 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
      901 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
      961 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     1021 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     1081 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     1141 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     1201 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     1261 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     1321 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     1381 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
     1441 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
     1501 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     1561 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     1621 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     1681 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     1741 ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac
     1801 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
     1861 gaaggagata taccatgggt tcttctcacc atcaccatca ccatggttct tctatgaaaa
     1921 tcgaagaagg taaactggta atctggatta acggcgataa aggctataac ggtctcgctg
     1981 aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata
     2041 aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac attatcttct
     2101 gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg
     2161 acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca
     2221 agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc
     2281 tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga
     2341 aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg
     2401 ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg
     2461 gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca
     2521 aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa
     2581 cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt
     2641 atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc
     2701 tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcaaaagag ttcctcgaaa
     2761 actatctgct gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg
     2821 tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc acgtattgcc gccactatgg
     2881 aaaacgccca gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg
     2941 ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga
     3001 aagacgcgca gactaatggg atcgaggaaa acctgtactt ccaatccaat gcaagaaata
     3061 aaattttcat aagccacgcc acaccagagg atgatgattt taccaggtgg ttatcgctaa
     3121 aactcattgg tctgggttat gaagtttggt gtgatatcct tttcttggac aagggagttg
     3181 acttttggag caccattgaa aaagagatac gggaaaacac ctgtaaattc ctaattgttt
     3241 cttccacagc aggaaataag cgggaaggtg tcttgaaaga actggcggta gcaacgaaag
     3301 ttaaaaagca ccttcaagat gatatgttca tcataccgct tgctattgat gaaaatctgt
     3361 cttatgatga catcaatatc gaaattgtcc gtttaaacgc catcgacttt aaaaaatcct
     3421 gggcaaaagg attgcaggac ttgctggacg cattcgaaaa gcagaatgtt ccaaaaaaac
     3481 ctcctgacca ttcaaaaagc aatttgcttt accaacaaat tttccttcat gacaagcaag
     3541 cgattgagaa ggaagaaacc tatgattcta actggttccc tattatttca tttcctaatg
     3601 aactaaggtt tcatagatac gattggcgtt tgccaaaaca atttgatgta agaaccctcg
     3661 catttccagc tattcgctac aaagagtatc tgtgcacgtt tgcatgggag tatgatttca
     3721 tacaccaatt accaaaaacc gaaacataca atggccagga atcaattaga atttctacaa
     3781 gtgatattct atcaggtagg tatgacactg attttatcag aaactacgaa tgtcaaagac
     3841 tgattgtcca gcttatcaac aaggcatttg agcttagaat gaaagataag aatgtaaggg
     3901 aatatcaaat gtcgaaaacc tttgcttatt ggattgaaaa aggcaaactg gaaaaagaca
     3961 aatttgaaaa gattaagtta gtcggcaaac agaaaaataa atactggcac tttgggattt
     4021 cggcagccgg taagctctat ccctctcccg ttttaatggt ttcgagtcat attatcttta
     4081 ccatggatgg tattaatctt atcaaatcta aaagcattca gcattcatct cgaagaaaac
     4141 agggtaagaa ttggtggaat gacaaatggc gggaaaaatt acttgccttt atccgatttc
     4201 tttcagatga tcaaaacgct atttacctaa atgtaggaag cgaagagaaa atcctgattt
     4261 caaataaacc tctgaaattt tttgggaaaa tgagttatgt aacgccttct gaagtaactt
     4321 tggaagaaga atcagtatta gcagatataa acaactttga agaagatacg gaagatttag
     4381 acgaattaga agatattgaa taactcgaag cttagatttt aaataacaat actaaggagg
     4441 aattacatat gaaggaattg atctacatag aagaaccctc tatcctcttt gctcatggtc
     4501 agaaatgtac tgatcctaga gatggattgg cattattcgg acctctgaat cagatatatg
     4561 gtattaaaag tggagtcgtt ggaacacaaa aaggtttgca gattttcaaa tcctatctgg
     4621 ataaaattca gaaaccaata tacaaccata acaatattac aagacccatg ttcccgggtt
     4681 ttgaagcagt ttttggctgc aagtgggaat ctcaaaacat tgtattcaaa gaaataactg
     4741 acgaagaaat tagaagatat ttgtttaatg ccagcacaca taaacggacg tatgatttgg
     4801 taacgctttt taatgacaaa attattaccg ccaataaaaa tgatgaagaa cgagttgatg
     4861 tttggtttgt cattgttcct gaagaaattt acaagtattg ccggcccaat tcagttcttc
     4921 caaatgagtt ggtccagacg aaatctctaa tctcgaaatc gaaagcaaag tcgtttcgat
     4981 atacaccaac gctattcgaa gagttcaaca agaaactgaa agaggtagag aaagaagcaa
     5041 aaacctataa ctacgatgca cagtttcacg atcagctaaa agctcgatta ctggaacata
     5101 cgattccaac tcaaatactg agagaaagca cattagcatg gagggatttt aagaacacct
     5161 ttggtgctcc aataagggat tttagtaaga ttgaaggtca tctggcctgg acaatttcaa
     5221 ctgctgcgta ctacaaagcc ggtggcaaac catggaaact tggtgacatc cgacccggag
     5281 tatgctattt aggattagtt tacaaaaaaa tagagaaaag taaaaatccc caaaatgcgt
     5341 gttgcgcagc tcaaatgttc ttggataacg gtgatggcac tgtctttaaa ggtgaagttg
     5401 gcccttggta caaccctgaa aagggcgaat atcatttgaa gcccaaggag gctaaagctc
     5461 ttttgactca agcattagaa tcgtacaagg aacaaaacaa aagctacccg aaagaagtat
     5521 tcatacatgc cagaactcgc tttaatgatg aggagtggaa tgcatttaat gaagtaaccc
     5581 caaaaaatac aaaccttgtt ggggtgacaa ttactaaatc aaaaccatta aaattataca
     5641 agacagaagg agcttttcca ataatgagag ggaatgcata tatagtggat gaaaaaaaag
     5701 cgtttttatg gacattaggt tttgttccta aactgcaatc tactctttca atggaagttc
     5761 cgaatcctat tttcattgaa ataaataaag gagaagccga aattcaacaa gtattgaagg
     5821 acatacttgc attaacaaaa ttgaattaca atgcttgtat ttatgcagac ggagaaccag
     5881 tgacacttcg ttttgcgaat aaaatcggag aaatacttac ggccagtaca gaaataaaaa
     5941 caccaccact tgcatttaaa tactacatat aataacattg gaagtggata acggatccga
     6001 attcgagcgc cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag
     6061 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     6121 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
     6181 gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6421 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6541 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6601 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaactagtaa cgtttacaat
     6661 ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6721 acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat
     6781 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     6841 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     6901 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     6961 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     7021 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     7081 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     7141 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     7201 gcgcatcaac aatgttttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     7261 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     7321 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     7381 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     7441 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     7501 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa
     7561 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     7621 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7681 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7741 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7801 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7861 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7921 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7981 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8041 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8101 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8161 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8221 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8281 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     8341 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     8401 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8461 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
     8521 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     8581 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     8641 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8701 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
     8761 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
     8821 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     8881 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
     8941 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
     9001 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
     9061 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
     9121 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     9181 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     9241 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     9301 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     9361 ccgtggggcc gccatgccgg cgataatg
//
