LOCUS       sequence-36010-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Agarwala S, Sanders TA, Ragsdale CW
  TITLE     Sonic hedgehog control of size and shape in midbrain pattern 
            formation.
  JOURNAL   Science. 2001 Mar 16. 291(5511):2147-50.
  PUBMED    11251119
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2001 Mar 16. 291(5511):2147-50."
COMMENT     Sequence Label: pEFX-AP
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtcgtgagga ttagcttggt actaatacga ctcactatag ggagacccaa gctggctagg
       61 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcct gcctcgccac
      121 tgtcctgctg ccctccagac atgctggggc cctgcatgct gctgctgctg ctgctgctgg
      181 gcctgaggct acagctctcc ctgggcatca tcccagttga ggaggagaac ccggacttct
      241 ggaaccgcga ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag
      301 ccgccaagaa cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg
      361 ccaggatcct aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg
      421 accgcttccc atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca
      481 gtggagccac agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct
      541 tgagtgcagc cgcccgcttt aaccagtgcn ncacgacacg cggcaacgag gtcatctccg
      601 tgatgaatcg ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc
      661 agcacgcctc gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg
      721 ccgacgtgcc tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct
      781 ccaacatgga cattgacgtg atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa
      841 ccccagaccc tgagtaccca gatgactaca gcca
//
