LOCUS       sequence-360102-        7676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV construct to ubiquitously express mouse Cyp26a1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Amamoto R, Wallick GK, Cepko CL
  TITLE     Retinoic acid signaling mediates peripheral cone photoreceptor 
            survival in a mouse model of retina degeneration.
  JOURNAL   Elife. 2022 Mar 22;11. pii: 76389. doi: 10.7554/eLife.76389.
  PUBMED    35315776
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-22"; volume: "11. pii: 76389. doi"; pages: " 
            10.7554/eLife.76389"
COMMENT     Sequence Label: AAV-CMV-Cyp26a1
FEATURES             Location/Qualifiers
     source          1..7676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   7..136
                     /label=AAV2 ITR
     primer_bind     complement(192..211)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        313..692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        693..896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     847..867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     893..917
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1010..1485
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(3054..3070)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3055..3071)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3164..3558
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      4460..4973
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4547..4566)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4757..4778
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5454..5473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5573..5595
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5633..5651)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5719..5823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5824..5892,5893..6684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6042..6061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6855..7443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7344..7363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttggcc
      241 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
      301 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      361 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      421 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      481 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      541 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      601 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      661 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      721 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      781 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      841 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
      901 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      961 accgggaccg atccagcctc ccctcgaagc tgatcctgag aacttcaggg tgagtctatg
     1021 ggacccttga tgttttcttt ccccttcttt tctatggtta agttcatgtc ataggaaggg
     1081 gagaagtaac agggtacaca tattgaccaa atcagggtaa ttttgcattt gtaattttaa
     1141 aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata ctttccctaa
     1201 tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac cattctaaag
     1261 aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat aaatatttct
     1321 gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct acaatccagc
     1381 taccattctg cttttatttt atggttggga taaggctgga ttattctgag tccaagctag
     1441 gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct gggcaacgtg
     1501 ctggtctgtg tgctggccca tcactttggc aaagaattcc gcgggcccgg gatccaatgg
     1561 ggctcccggc gctgctggcc agtgcgctct gcaccttcgt gctgccgctg ctgctcttcc
     1621 tggcggcgct caagctctgg gacctgtact gtgtgagcag ccgcgatcgc agctgcgccc
     1681 tccccttgcc ccccggtacc atgggcttcc cattctttgg ggaaacattg cagatggtgc
     1741 ttcagcggag gaagtttctg cagatgaagc gcaggaaata cggcttcatc tacaagacgc
     1801 atctgtttgg gcggcccacg gtgcgggtga tgggcgcgga taatgtgcgg cgcatcttgc
     1861 tgggagagca ccggttggtg tcggtgcact ggcccgcgtc ggtgcgcacc atcctgggcg
     1921 ctggctgcct ctccaacctg cacgattcct cgcacaagca gcgaaagaag gtgattatgc
     1981 aggccttcag ccgcgaggca ctccagtgct acgtgcccgt gatcgctgag gaagtcagca
     2041 gttgtctgga gcagtggcta agctgcggcg agcgcggcct cctggtctac cccgaggtga
     2101 agcgcctcat gttccgcatc gccatgcgca tcctgctggg ctgcgagccg ggtccagcgg
     2161 gcggcgggga ggacgagcag cagctcgtgg aggctttcga ggagatgacc cgcaatctct
     2221 tctctcttcc cattgacgtg ccctttagcg gcctgtaccg gggcgtgaag gcgcggaacc
     2281 ttatacacgc gcgcatcgag gagaacattc gcgccaagat ccgccggctt caggctacag
     2341 agccggatgg gggttgcaag gacgcgctgc agctcctgat tgagcactcg tgggagaggg
     2401 gagagaggct ggatatgcag gcactaaaac aatcgtcaac agagctcctc tttggtggtc
     2461 atgaaactac agccagtgct gcgacgtcac tgatcactta cctaggactc tacccacatg
     2521 tcctccagaa agttcgagaa gagataaaga gcaagggctt actttgcaag agcaatcaag
     2581 acaacaagtt agacatggaa actttggaac agcttaaata cactgggtgt gtcattaagg
     2641 agaccctgcg attgaatcct ccggttccag gagggtttcg ggttgctctg aagacttttg
     2701 agctgaatgg ataccagatc cccaagggct ggaatgttat ttacagtatc tgtgacaccc
     2761 acgatgtggc agatatcttc actaacaagg aggaatttaa tcccgaccgc tttatagtgc
     2821 ctcatccaga ggatgcttcc cggttcagct tcattccatt tggaggaggc cttcggagct
     2881 gtgtaggcaa agagtttgca aaaattcttc ttaagatatt tacagtggag ctggctaggc
     2941 actgtgattg gcagcttcta aatggacctc ctacaatgaa gacaagcccc actgtgtacc
     3001 ctgtggacaa tctccctgca agatttaccc acttccaggg agatatctga tatcgatacc
     3061 gtcgacctcg agggcccaga tctaattcac cccaccagtg caggctgcct atcagaaagt
     3121 ggtggctggt gtggctaatg ccctggccca caagtatcac taagctcgct ttcttgctgt
     3181 ccaatttcta ttaaaggttc ctttgttccc taagtccaac tactaaactg ggggatatta
     3241 tgaagggcct tgagcatctg gattctgcct aataaaaaac atttattttc attgcaatga
     3301 tgtatttaaa ttatttctga atattttact aaaaagggaa tgtgggaggt cagtgcattt
     3361 aaaacataaa gaaatgaaga gctagttcaa accttgggaa aatacactat atcttaaact
     3421 ccatgaaaga aggtgaggct gcaaacagct aatgcacatt ggcaacagcc cctgatgcct
     3481 atgccttatt catccctcag aaaaggattc aagtagaggc ttgatttgga ggttaaagtt
     3541 ttgctatgct gtattttaca ttacttattg ttttagctgt cctcatgaat gtcttttcac
     3601 tacccatttg cttatcctgc atctctcagc cttgactcca ctcagttctc ttgcttagag
     3661 ataccacctt tcccctgaag tgttccttcc atgttttacg gcgagatggt ttctcctcgc
     3721 ctggccactc agccttagtt gtctctgttg tcttatagag gtctacttga agaaggaaaa
     3781 acagggggca tggtttgact gtcctgtgag cccttcttcc ctgcctcccc cactcacagt
     3841 gacccggaat ccctcgacat ctagagcatg gctacgtaga taagtagcat ggcgggttaa
     3901 tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3961 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     4021 cagtgagcga gcgagcgcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4081 tcccaacagt tgcgcagcct gaatggcgaa tggaattcca gacgattgag cgtcaaaatg
     4141 taggtatttc catgagcgtt tttcctgttg caatggctgg cggtaatatt gttctggata
     4201 ttaccagcaa ggccgatagt ttgagttctt ctactcaggc aagtgatgtt attactaatc
     4261 aaagaagtat tgcgacaacg gttaatttgc gtgatggaca gactctttta ctcggtggcc
     4321 tcactgatta taaaaacact tctcaggatt ctggcgtacc gttcctgtct aaaatccctt
     4381 taatcggcct cctgtttagc tcccgctctg attctaacga ggaaagcacg ttatacgtgc
     4441 tcgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4501 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4561 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4621 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4681 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4741 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4801 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4861 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttaaata
     4921 tttgcttata caatcttcct gtttttgggg cttttctgat tatcaaccgg ggtacatatg
     4981 attgacatgc tagttttacg attaccgttc atcgattctc ttgtttgctc cagactctca
     5041 ggcaatgacc tgatagcctt tgtagagacc tctcaaaaat agctaccctc tccggcatga
     5101 atttatcagc tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt
     5161 ctcacccgtt tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg
     5221 gttctaaaaa tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg
     5281 gtcataatgt ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt
     5341 ttgctaattc tttgccttgc ctgtatgatt tattggatgt tggaattcct gatgcggtat
     5401 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     5461 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5521 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5581 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5641 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     5701 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5761 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5821 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5881 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5941 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6001 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6061 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6121 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6181 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6241 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6301 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6361 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6421 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6481 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6541 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6601 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6661 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6721 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6781 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6841 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6901 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6961 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7021 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7081 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7141 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7201 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7261 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7321 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7381 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7441 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7501 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7561 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7621 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatg
//
