LOCUS       sequence-360115-       14228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the rat TrkC fused in the C-terminus with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14228)
  TITLE     Neurotrophin related plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLZRS-TrkC-mCherry
FEATURES             Location/Qualifiers
     source          1..14228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(89..193)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             730..1199
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1264..1621
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1686..2102
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2032..2054
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2070..2086
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2112..2486
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2424..2440
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     2525..2541
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2525..2541
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             5203..5913
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5348..5366)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5616..5635
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             6121..6590
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6720..6737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6891..7479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6971..6990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7602..7736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7626..7645)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7680..7699
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7966..8565)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8049..8069)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8546..8565
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8648..9147)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8696..8715)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     9089..9112
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      11173..12962
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(13456..14228,1..19,20..88))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14079..14098
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
       61 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      121 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
      181 taggggttcc gcgcacattt ccccgaaaag tgccaccagc tttgctctta ggagtttcct
      241 aatacatccc aaactcaaat atataaagca tttgacttgt tctatgccct agggggcggg
      301 gggaagctaa gccagctttt tttaacattt aaaatgttaa ttccatttta aatgcacaga
      361 tgtttttatt tcataagggt ttcaatgtgc atgaatgctg caatattcct gttaccaaag
      421 ctagtataaa taaaaataga taaacgtgga aattacttag agtttctgtc attaacgttt
      481 ccttcctcag ttgacaacat aaatgcgctg ctgagaagcc agtttgcatc tgtcaggatc
      541 aatttcccat tatgccagtc atattaatta ctagtcaatt agttgatttt tatttttgac
      601 atatacatgt gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
      661 gcaaggcatg gaaaaataca taactgagaa tagaaaagtt cagatcaagg tcaggaacag
      721 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
      781 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
      841 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
      901 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
      961 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     1021 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg attgactgag
     1081 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     1141 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
     1201 ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga
     1261 ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt
     1321 tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg
     1381 aactgacgag ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg acttcggggg
     1441 ccgtttttgt ggcccgacct gagtccaaaa atcccgatcg ttttggactc tttggtgcac
     1501 cccccttaga ggagggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
     1561 ctccgtctga atttttgctt tcggtttggg accgaagccg cgccgcgcgt cttgtctgct
     1621 gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaatatgg
     1681 gcccgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg
     1741 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct
     1801 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc
     1861 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg
     1921 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct
     1981 ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac
     2041 ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg
     2101 cccccatatg gccatatgag atcttatatg gggcaccccc gccccttgta aacttccctg
     2161 accctgacat gacaagagtt actaacagcc cctctctcca agctcactta caggctctct
     2221 acttagtcca gcacgaagtc tggagacctc tggcggcagc ctaccaagaa caactggacc
     2281 gaccggtggt acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga
     2341 ctaagaacct agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg
     2401 ccctcaaagt agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc
     2461 ccgggggtgg accatcctct agactgccgg atcccagtgt ggtggtacgg gaattcgcgg
     2521 ccgctctaga actagtggat cccccgggct gcaggaattc ggcacgagct tttttattat
     2581 tattattttt gccttccccc ctccctcgct gtctttgttt ccttcccccc ctgctctccc
     2641 cactcccccc accccacccc cctctcctct cctcctcctc ctctttttta gaagcagcga
     2701 tcggagatgg atgtctctct ttgcccagcc aagtgtagtt tctggcggat tttcttgctg
     2761 ggaagcgtct ggctggacta tgtgggctcc gtgctggctt gccctgcaaa ttgtgtctgc
     2821 agcaagactg agatcaattg ccggcggccg gacgatggga acctctttcc cctcctggaa
     2881 gggcaggatt cagggaacag caatgggaac gccagcatca acatcacgga catctcaagg
     2941 aatatcactt ccatacacat agagaactgg cggggcctgc acacactcaa tgctgtggat
     3001 atggagctct acacgggact ccagaagctg accatcaaga attctggact ccggaatatt
     3061 cagcccagag cctttgctaa gaatccccac ttgcgttata taaacttgtc aagtaaccgg
     3121 ctcaccacac tctcctggca gctcttccag acgctgagtc ttcgggaatt gagactggag
     3181 cagaacttct tcaactgcag ctgtgacatc cgctggatgc agctgtggca ggaacagggg
     3241 gaggcacggc tggacagcca gagcctctac tgcatcagtg ctgatggctc ccaactcccc
     3301 ctcttccgca tgaacatcag tcagtgtgat ctccctgaga tcagtgtgag tcacgtgaac
     3361 ctgactgtcc gagaaggaga caatgctgta atcacttgta atggctctgg ctctcctttg
     3421 cctgatgtgg actggatagt caccggactg cagtccatca atactcatca gaccaatctg
     3481 aactggacca atgtacatgc catcaacttg accctggtga atgtgacgag cgaagacaat
     3541 ggtttcaccc tgacgtgcat tgcagagaac gtggtgggca tgagcaatgc cagcgttgct
     3601 ctcactgtct actaccctcc acgtgtggtg agcttggtgg agcctgaggt acgcctggaa
     3661 cattgcattg agtttgtggt gcgtggcaac ccgacaccca cgctgcattg gctgtacaat
     3721 ggacagccac tgagggaatc caagatcatt catatggact actaccagga gggtgaagtc
     3781 tcagagggct gcctgctctt caacaagccc acccactaca acaatggcaa ctacaccctc
     3841 attgctaaga atgccctggg cacagccaac cagaccatca acggccactt ccttaaggag
     3901 ccctttccag agagcacaga tttctttgac tttgagtctg atgcgagccc tacacctcct
     3961 atcactgtga cccacaaacc agaggaagac acttttgggg tgtccatagc agttggactt
     4021 gctgcttttg cctgcgtcct tctggtggtc ctctttatca tgatcaacaa gtacggtcga
     4081 cggtccaaat ttggaatgaa gggtcctgtg gctgttatca gtggggagga ggactcagcc
     4141 agcccactgc accatatcaa ccatggcatc actacacctt catcgctgga tgctgggccg
     4201 gatacagtgg tcattggcat gactcgaatt ccagtcattg agaaccccca atatttccgt
     4261 cagggacaca attgccacaa gccagacaca tatgttcaac acatcaagag gagagacatc
     4321 gtgttgaaga gagaactggg tgagggagcc tttggaaagg tcttcctggc tgagtgctac
     4381 aatctaagcc ccaccaaaga caagatgctt gtggcagtga aggccctgaa ggatcccacc
     4441 ttggctgcca ggaaggattt ccagagggag gccgagctgc tcactaacct gcagcatgag
     4501 catattgtca aattctatgg ggtctgtggt gatggggatc cactcatcat ggtctttgaa
     4561 tacatgaagc atggagacct gaacaagttc ctcagggccc atgggccaga tgcgatgatc
     4621 cttgtggatg gacagccacg ccaggccaag ggggagctag gactctccca gatgctccac
     4681 atcgccagtc agatagcctc tggcatggtg tacttggcct cccagcactt tgtgcaccgg
     4741 gacctggcca ccaggaactg cctagttgga gccaaccttc tagtgaagat tggagatttt
     4801 ggcatgtcca gggacgtcta cagtactgat tactacaggg tgggaggaca caccatgctc
     4861 cccatccgct ggatgccacc tgaaagcatc atgtaccgga agtttaccac agagagtgat
     4921 gtgtggagct ttggggttat tctttgggag atctttacct atgggaagca accatggttc
     4981 cagctctcta acacagaggt cattgaatgc atcacccaag gccgtgtctt ggagagaccc
     5041 cgagtctgcc caaaagaagt atatgatgtc atgctggggt gctggcagag ggaaccacaa
     5101 cagcgactga atattaagga gatctacaaa atcctccatg ctttggggaa ggccacccca
     5161 atctacctgg acattcttgg cgctgctgct tacgtactcg agatggtgag caagggcgag
     5221 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     5281 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     5341 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     5401 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     5461 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     5521 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     5581 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     5641 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     5701 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     5761 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     5821 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     5881 tccaccggcg gcatggacga gctgtacaag taagcggccg ctacgtactc gaggggccgc
     5941 cagcacagtg gtcgacgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     6001 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     6061 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     6121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     6181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     6241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     6301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     6361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     6421 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     6481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     6541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat
     6601 gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat
     6661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7561 tcaaaaagga tcttcaccta gatccttttg cggccgaatt agatccagac atgataagat
     7621 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     7681 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     7741 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     7801 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagag tcggtgggcc
     7861 tcgggggcgg gtgcggggtc ggcggggccg ccccgggtgg cttcggtcgg agccatgggg
     7921 tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg caccgggctt
     7981 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     8041 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     8101 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     8161 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     8221 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     8281 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     8341 gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     8401 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg ggtcgacggt
     8461 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg cccgggggac
     8521 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggaag gtcgtctcct
     8581 tgtgaggggt caggggcgtg ggtcagggga tggtggcggc accggtcgtg gcggccgacc
     8641 tgcaggtcga aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt
     8701 gaagcgtgca gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc
     8761 cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg
     8821 agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc
     8881 tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg
     8941 cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg
     9001 ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc
     9061 agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact
     9121 gccttgggaa aagcgcctcc cctacccggt agaattaatt ctcatgtttg acagcttatc
     9181 atcgataagc tgatcctcac aggccgcacc cagcttttct tccgttgccc cagtagcatc
     9241 tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat ccctaccgtc
     9301 cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg caaatcttga
     9361 ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg aggatggttc
     9421 ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag gggatgaggg
     9481 tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga gcgtcgtcta
     9541 ataaagatgt ctattgatct cttttagtgt gaatcatgtc tgacgagggg ccaggtacag
     9601 gacctggaaa tggcctagga gagaagggag acacatctgg accagaaggc tccggcggca
     9661 gtggacctca aagaagaggg ggtgataacc atggacgagg acggggaaga ggacgaggac
     9721 gaggaggcgg aagaccagga gccccgggcg gctcaggatc agggccaaga catagagatg
     9781 gtgtccggag accccaaaaa cgtccaagtt gcattggctg caaagggacc cacggtggaa
     9841 caggagcagg agcaggagcg ggaggggcag gagcagsagg rgcaggagsa ggrgsaggrg
     9901 caggagsrgs aggagcagga ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc
     9961 gaggaggtag tggaggccgg ggtcgaggag gtagtggagg ccgccggggt agaggacgtg
    10021 aaagagccag ggggggaagt cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa
    10081 agaggcccag gagtcccagt agtcagtcat catcatccgg gtctccaccg cgcaggcccc
    10141 ctccaggtag aaggccattt ttccaccctg taggggaagc cgattatttt gaataccacc
    10201 aagaaggtgg cccagatggt gagcctgacg tgcccccggg agcgatagag cagggccccg
    10261 cagatgaccc aggagaaggc ccaagcactg gaccccgggg tcagggtgat ggaggcaggc
    10321 gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg
    10381 agaacattgc agaaggttta agagctctcc tggctaggag tcacgtagaa aggactaccg
    10441 acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg tagtaagacc tccctttaca
    10501 acctaaggcg aggaactgcc cttgctattc cacaatgtcg tcttacacca ttgagtcgtc
    10561 tcccctttgg aatggcccct ggacccggcc cacaacctgg cccgctaagg gagtccattg
    10621 tctgttattt catggtcttt ttacaaactc atatatttgc tgaggttttg aaggatgcga
    10681 ttaaggacct tgttatgaca aagcccgctc ctacctgcaa tatcagggtg actgtgtgca
    10741 gctttgacga tggagtagat ttgcctccct ggtttccacc tatggtggaa ggggctgccg
    10801 cggagggtga tgacggagat gacggagatg aaggaggtga tggagatgag ggtgaggaag
    10861 ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc
    10921 cccgcactaa agaataaatc cccagtagac atcatgcgtg ctgttggtgt atttctggcc
    10981 atctgtcttg tcaccatttt cgtcctccca acatggggca attgggcata cccatgttgt
    11041 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac
    11101 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat
    11161 gggagcaacc agcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
    11221 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
    11281 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
    11341 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
    11401 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
    11461 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta cccagatata
    11521 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt
    11581 aggatagcat atactaccct aatctctatt aggatagcat atgctacccg gatacagatt
    11641 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
    11701 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
    11761 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
    11821 aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc
    11881 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
    11941 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
    12001 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
    12061 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
    12121 aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa
    12181 agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg
    12241 ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg
    12301 ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt
    12361 aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt
    12421 gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat
    12481 attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt gagggttata
    12541 ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt
    12601 cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt
    12661 attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg
    12721 cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga
    12781 atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc
    12841 ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc
    12901 tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg
    12961 tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct
    13021 tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg
    13081 ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac
    13141 ataaaggcaa tgttgtgttg cagtccacag actgcaaagt ctgctccagg atgaaagcca
    13201 ctcagtgttg gcaaatgtgc acatccattt ataaggatgt caactacagt cagagaaccc
    13261 ctttgtgttt ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac
    13321 caaccaactg aagggattac atgcactgcc ccgcgaagaa ggggcagaga tgtcgtagtc
    13381 aggtttagtt cgtccggggc ggggatctgg ccgcaaatca atctaaagta tatatgagta
    13441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    13501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    13561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    13621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    13681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    13741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    13801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    13861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    13921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    14041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    14101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    14161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    14221 ttttactt
//
