LOCUS       sequence-360131-       13673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for expression of Plexin-B2 locked ring mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Junqueira Alves C, Hannah T, Sadia S, Kolsteeg C, Dixon A, Wiener 
            RJ, Nguyen H, Tipping MJ, Silva Ladeira J, Fernandes da Costa 
            Franklin P, de Paula Dutra de Nigro N, Alves Dias R, Zabala Capriles
            PV, Rodrigues Furtado de Mendonca JP, Slesinger PA, Costa KD, Zou H,
            Friedel RH
  TITLE     Invasion of glioma cells through confined space requires membrane 
            tension regulation and mechano-electrical coupling via Plexin-B2.
  JOURNAL   Nat Commun. 2025 Jan 2;16(1):272. doi: 10.1038/s41467-024-55056-6.
  PUBMED    39747004
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-2"; volume: "16(1):272. doi"; pages: " 
            10.1038/s41467-024-55056-6"
COMMENT     Sequence Label: pLV-PB2_Lock1-I436C+S993C
FEATURES             Location/Qualifiers
     source          1..13673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     570..589
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             791..1024
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1096..1217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1133..1152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1187..1206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1257..1392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1412..1431)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1413..1431)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1441..1458)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1441..1457)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1450..1472)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1599..2054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1686..1705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1896..1917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2080..2184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2185..2253,2254..3045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2403..2422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3216..3804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3705..3724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3958..3975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4092..4113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4128..4133,4134..4151,4152..4158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4166..4182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4171..4193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4190..4206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4190..4206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4225..4245
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4227..4245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4273..4499
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4500..4680
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4727..4852
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5345..5578
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5763..5807
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6074..6191
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6237..6747
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6666..6684
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    6802..6826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12371..12395)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    12426..13014
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12479..12499)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12897..12908)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        13057..13556
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13092..13115)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     13489..13508
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(13570..13673,1..700)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13633..13652)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
       61 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
      121 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      181 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      241 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      301 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      361 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      421 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
      481 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
      541 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
      601 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
      661 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatccgt cgagggcccg
      721 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
      781 aaggggggac tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt
      841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     1021 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     1081 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1201 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     1261 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     1321 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     1381 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     1441 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     1501 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     1561 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     1621 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1681 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1741 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     1801 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     1861 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     1921 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     1981 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2041 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     2101 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     2161 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     2221 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     2281 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     2341 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     2401 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     2461 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     2521 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     2581 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     2641 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     2701 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     2761 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2821 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2881 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2941 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3001 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     3061 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3121 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3181 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     3241 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     3301 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3361 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3421 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3481 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3541 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3601 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3661 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3721 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3781 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3841 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3901 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3961 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     4021 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     4081 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     4141 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     4201 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     4261 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     4321 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     4381 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     4441 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     4501 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4561 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4621 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4681 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     4741 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     4801 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     4861 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     4921 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     4981 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     5041 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     5101 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     5161 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     5221 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     5281 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     5341 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     5401 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     5461 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     5521 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     5581 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     5641 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     5701 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     5761 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     5821 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     5881 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     5941 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     6001 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     6061 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     6121 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     6181 ttcaaaattt tatcgatcac gagactagcc tcgagaagct tgatatcgaa ttccacgggg
     6241 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     6301 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     6361 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     6421 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     6481 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     6541 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     6601 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     6661 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     6721 gaccgaatca ccgacctctc tccccagggg gatccaccgg ttcgtcgact agtccagtgt
     6781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag cagaagggcc gtcaaggccc
     6841 accatggcac tgcagctctg ggccctgacc ctgctgggcc tgctgggcgc aggtgccagc
     6901 ctgaggcccc gcaagctgga cttcttccgc agcgagaaag agctgaacca cctggctgtg
     6961 gatgaggcct caggcgtggt gtacctgggg gcggtgaatg ccctctacca gctggatgcg
     7021 aagctgcagc tggagcagca ggtggccacg ggcccggccc tggacaacaa gaagtgcacg
     7081 ccgcccatcg aggccagcca gtgccatgag gctgagatga ctgacaatgt caaccagctg
     7141 ctgctgctcg accctcccag gaagcgcctg gtggagtgcg gcagcctctt caagggcatc
     7201 tgcgctctgc gcgccctgag caacatctcc ctccgcctgt tctacgagga cggcagcggg
     7261 gagaagtctt tcgtggccag caatgatgag ggcgtggcca cagtggggct ggtgagctcc
     7321 acgggtcctg gtggtgaccg cgtgctgttt gtgggcaaag gcaatgggcc acacgacaac
     7381 ggcatcatcg tgagcactcg gctgttggac cggactgaca gcagggaggc ctttgaagcc
     7441 tacacggacc acgccaccta caaggccggc tacctgtcca ccaacacaca gcagttcgtg
     7501 gcggccttcg aggacggccc ctacgtcttc tttgtcttca accagcagga caagcacccg
     7561 gcccggaacc gcacgctgct ggcacgcatg tgcagagaag accccaacta ctactcctac
     7621 ctggagatgg acctgcagtg ccgggacccc gacatccacg ccgctgcctt tggcacctgc
     7681 ctggccgcct ccgtggctgc gcctggctct ggcagggtgc tatatgctgt cttcagcaga
     7741 gacagccgga gcagtggggg gcccggtgcg ggcctctgcc tgttcccgct ggacaaggtg
     7801 cacgccaaga tggaggccaa ccgcaacgcc tgttacacag gcacccggga ggcccgtgac
     7861 atcttctaca agcccttcca cggcgatatc cagtgcggcg gccacgcgcc gggctccagc
     7921 aagagcttcc catgtggctc ggagcacctg ccctacccgc tgggcagccg cgacgggctc
     7981 agaggcacag ccgtgctgca gcgtggaggc ctgaacctca cggctgtcac cgtggctgct
     8041 gaaaataacc acactgttgc ttttctgggc acctctgatg gccggatcct caaggtgtac
     8101 ctcaccccag atggcacctc ctcagagtac gactctatcc ttgtggagtg taacaagaga
     8161 gtcaagcgcg acctggtact gtctggagac ctgggcagcc tgtacgccat gacccaggac
     8221 aaggtgttcc ggctgccggt gcaggagtgc ctgagctacc cgacctgcac ccagtgccgc
     8281 gactcccagg acccctactg cggctggtgc gtcgtcgagg gacgatgcac ccggaaggcc
     8341 gagtgtccgc gggccgagga ggccagccac tggctgtgga gccgaagcaa gtcctgcgtg
     8401 gccgtcacca gcgcccagcc acagaacatg agccggcggg cccaggggga ggtgcagctg
     8461 accgtcagcc ccctccctgc cctgagcgag gaggacgagt tgctgtgcct ttttggggag
     8521 tcgccgccac accccgcccg cgtggagggc gaggccgtca tctgcaactc cccaagcagc
     8581 atccccgtca caccgccagg ccaggaccac gtggccgtga ccatccagct cctccttaga
     8641 cgaggcaaca tcttcctcac gtcctaccag taccccttct acgactgccg ccaggccatg
     8701 agcctggagg agaacctgcc gtgcatctcc tgcgtgagca accgctggac ctgccagtgg
     8761 gacctgcgct accacgagtg ccgggaggct tcgcccaacc ctgaggacgg catcgtccgt
     8821 gcccacatgg aggacagctg tccccagttc ctgggaccca gccccctggt gatccccatg
     8881 aaccacgaga cagatgtgaa cttccagggc aagaacctgg acaccgtgaa gggttcctcc
     8941 ctgcacgtgg gcagtgactt gctcaagttc atggagccgg tgaccatgca ggaatctggg
     9001 accttcgcct ttcggacccc aaagctgtcc cacgatgcca acgagacgct gcccctgcac
     9061 ctctacgtca agtcttacgg caagaatatc gacagcaagc tccatgtgac cctctacaac
     9121 tgctcctttg gccgcagcga ctgcagcctg tgccgggccg ctaaccccga ctacaggtgt
     9181 gcgtggtgcg ggggccagag caggtgcgtg tatgaggccc tgtgcaacac cacctccgag
     9241 tgcccgccgc ccgtcatcac caggatccag cctgagacgg gccccctggg tgggggcatc
     9301 cgcatcacca tcctggggtc caatttgggc gtccaagcag gggacatcca gaggatctct
     9361 gtggccggcc ggaactgctc ctttcagccg gaacgttact ccgtgtccac ccggatcgtg
     9421 tgtgtgatcg aggctgcgga gacgcctttc acggggggtg tcgaggtgga cgtcttcggg
     9481 aaactgggcc gttcgcctcc caatgtccag ttcaccttcc aacagcccaa gcctctcagt
     9541 gtggagccgc agcagggacc gcaggcgggc ggcaccacac tgaccatcca cggcacccac
     9601 ctggacacgg gctcccagga ggacgtgcgg gtgaccctca acggcgtccc gtgtaaagtg
     9661 acgaagtttg gggcgcagct ccagtgtgtc actggccccc aggcgacacg gggccagatg
     9721 cttctggagg tctcctacgg ggggtccccc gtgcccaacc ccggcatctt cttcacctac
     9781 cgcgaaaacc ccgtactgcg agccttcgag ccgctacgat gctttgccag tggtggccgc
     9841 agcatcaacg tcacgggtca gggcttcagc ctgatccaga ggtttgccat ggtggtcatc
     9901 gcggagcccc tgcagtcctg gcagccgccg cgggaggctg aatccctgca gcccatgacg
     9961 gtggtgggta cagactacgt gttccacaat gacaccaagg tcgtcttcct gtccccggct
    10021 gtgcctgagg agccagaggc ctacaacctc acggtgctga tcgagatgga cgggcaccgt
    10081 gccctgctca gaacagaggc cggggccttc gagtacgtgc ctgaccccac ctttgagaac
    10141 ttcacaggtg gcgtcaagaa gcaggtcaac aagctcatcc acgcccgggg caccaatctg
    10201 aacaaggcga tgacgctgca ggaggccgag gccttcgtgg gtgccgagcg ctgcaccatg
    10261 aagacgctga cggagaccga cctgtactgt gagcccccgg aggtgcagcc cccgcccaag
    10321 cggcggcaga aacgagacac cacacacaac ctgcccgagt tcattgtgaa gttcggctct
    10381 cgcgagtggg tgctgggccg cgtggagtac gacacacggg tgagcgacgt gccgctcagc
    10441 ctcatcttgc cgctggtcat cgtgcccatg gtggtcgtca tcgcggtgtc tgtctactgc
    10501 tactggagga agagccagca ggccgaacga gagtatgaga agatcaagtc ccagctggag
    10561 ggcctggagg agagcgtgcg ggaccgctgc aagaaggaat tcacagacct gatgatcgag
    10621 atggaggacc agaccaacga cgtgcacgag gccggcatcc ccgtgctgga ctacaagacc
    10681 tacaccgacc gcgtcttctt cctgccctcc aaggacggcg acaaggacgt gatgatcacc
    10741 ggcaagctgg acatccctga gccgcggcgg ccggtggtgg agcaggccct ctaccagttc
    10801 tccaacctgc tgaacagcaa gtctttcctc atcaatttca tccacaccct ggagaaccag
    10861 cgggagttct cggcccgcgc caaggtctac ttcgcgtccc tgctgacggt ggcgctgcac
    10921 gggaaactgg agtactacac ggacatcatg cacacgctct tcctggagct cctggagcag
    10981 tacgtggtgg ccaagaaccc caagctgatg ctgcgcaggt ctgagactgt ggtggagagg
    11041 atgctgtcca actggatgtc catctgcctg taccagtacc tcaaggacag tgccggggag
    11101 cccctgtaca agctcttcaa ggccatcaaa catcaggtgg aaaagggccc ggtggatgcg
    11161 gtacagaaga aggccaagta cactctcaac gacacggggc tgctggggga tgatgtggag
    11221 tacgcacccc tgacggtgag cgtgatcgtg caggacgagg gagtggacgc catcccggtg
    11281 aaggtcctca actgtgacac catctcccag gtcaaggaga agatcattga ccaggtgtac
    11341 cgtgggcagc cctgctcctg ctggcccagg ccagacagcg tggtcctgga gtggcgtccg
    11401 ggctccacag cgcagatcct gtcggacctg gacctgacgt cacagcggga gggccggtgg
    11461 aagcgcgtca acacccttat gcactacaat gtccgggatg gagccaccct catcctgtcc
    11521 aaggtggggg tctcccagca gccggaggac agccagcagg acctgcctgg ggagcgccat
    11581 gccctcctgg aggaggagaa ccgggtgtgg cacctggtgc ggccgaccga cgaggtggac
    11641 gagggcaagt ccaagagagg cagcgtgaaa gagaaggagc ggacgaaggc catcaccgag
    11701 atctacctga cgcggctgct ctcagtcaag ggcacactgc agcagtttgt ggacaacttc
    11761 ttccagagcg tgctggcgcc tgggcacgcg gtgccacctg cagtcaagta cttcttcgac
    11821 ttcctggacg agcaggcaga gaagcacaac atccaggatg aagacaccat ccacatctgg
    11881 aagacgaaca gcttaccgct ccggttctgg gtgaacatcc tcaagaaccc ccacttcatc
    11941 tttgacgtgc atgtccacga ggtggtggac gcctcgctgt cagtcatcgc gcagaccttc
    12001 atggatgcct gcacgcgcac ggagcataag ctgagccgcg attctcccag caacaagctg
    12061 ctgtacgcca aggagatctc cacctacaag aagatggtgg aggattacta caaggggatc
    12121 cggcagatgg tgcaggtcag cgaccaggac atgaacacac acctggcaga gatttcccgg
    12181 gcgcacacgg actccttgaa caccctcgtg gcactccacc agctctacca atacacgcag
    12241 aagtactatg acgagatcat caatgccttg gaggaggatc ctgccgccca gaagatgcag
    12301 ctggccttcc gcctgcagca gattgccgct gcactggaga acaaggtcac tgacctctag
    12361 ggcctcatgg gcccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
    12421 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
    12481 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
    12541 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
    12601 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
    12661 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
    12721 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
    12781 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
    12841 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
    12901 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
    12961 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
    13021 ttctgcagat atccggacta gtgatctaat tctaccgggt aggggaggcg cttttcccaa
    13081 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
    13141 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
    13201 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
    13261 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
    13321 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
    13381 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
    13441 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
    13501 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
    13561 gcagccaata tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct
    13621 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctc
//
