LOCUS       sequence-360133-       13673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for expression of Plexin-B2 locked ring mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Junqueira Alves C, Hannah T, Sadia S, Kolsteeg C, Dixon A, Wiener 
            RJ, Nguyen H, Tipping MJ, Silva Ladeira J, Fernandes da Costa 
            Franklin P, de Paula Dutra de Nigro N, Alves Dias R, Zabala Capriles
            PV, Rodrigues Furtado de Mendonca JP, Slesinger PA, Costa KD, Zou H,
            Friedel RH
  TITLE     Invasion of glioma cells through confined space requires membrane 
            tension regulation and mechano-electrical coupling via Plexin-B2.
  JOURNAL   Nat Commun. 2025 Jan 2;16(1):272. doi: 10.1038/s41467-024-55056-6.
  PUBMED    39747004
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-2"; volume: "16(1):272. doi"; pages: " 
            10.1038/s41467-024-55056-6"
COMMENT     Sequence Label: pLV-PB2_Lock2-I436C+T1051C
FEATURES             Location/Qualifiers
     source          1..13673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    64..181
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        227..737
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     656..674
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    792..816
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6361..6385)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6416..7004
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6469..6489)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6887..6898)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7047..7546
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7082..7105)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7479..7498
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7560..8363
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7623..7642)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8233..8252
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8454..8687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8759..8880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8796..8815)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8850..8869
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8920..9055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9075..9094)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9076..9094)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9104..9121)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9104..9120)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9113..9135)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9262..9717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9349..9368)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9559..9580
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9743..9847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9848..9916,9917..10708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10066..10085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10879..11467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11368..11387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11621..11638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11755..11776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11791..11796,11797..11814,11815..11821)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11829..11845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11834..11856
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11853..11869
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11853..11869
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11888..11908
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11890..11908
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11936..12162
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             12163..12343
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12390..12515
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13008..13241
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13426..13470
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
       61 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      121 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      181 tatcgatcac gagactagcc tcgagaagct tgatatcgaa ttccacgggg ttggggttgc
      241 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
      301 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
      361 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
      421 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
      481 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
      541 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
      601 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
      661 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
      721 ccgacctctc tccccagggg gatccaccgg ttcgtcgact agtccagtgt ggtggaattc
      781 tgcagatatc aacaagtttg tacaaaaaag cagaagggcc gtcaaggccc accatggcac
      841 tgcagctctg ggccctgacc ctgctgggcc tgctgggcgc aggtgccagc ctgaggcccc
      901 gcaagctgga cttcttccgc agcgagaaag agctgaacca cctggctgtg gatgaggcct
      961 caggcgtggt gtacctgggg gcggtgaatg ccctctacca gctggatgcg aagctgcagc
     1021 tggagcagca ggtggccacg ggcccggccc tggacaacaa gaagtgcacg ccgcccatcg
     1081 aggccagcca gtgccatgag gctgagatga ctgacaatgt caaccagctg ctgctgctcg
     1141 accctcccag gaagcgcctg gtggagtgcg gcagcctctt caagggcatc tgcgctctgc
     1201 gcgccctgag caacatctcc ctccgcctgt tctacgagga cggcagcggg gagaagtctt
     1261 tcgtggccag caatgatgag ggcgtggcca cagtggggct ggtgagctcc acgggtcctg
     1321 gtggtgaccg cgtgctgttt gtgggcaaag gcaatgggcc acacgacaac ggcatcatcg
     1381 tgagcactcg gctgttggac cggactgaca gcagggaggc ctttgaagcc tacacggacc
     1441 acgccaccta caaggccggc tacctgtcca ccaacacaca gcagttcgtg gcggccttcg
     1501 aggacggccc ctacgtcttc tttgtcttca accagcagga caagcacccg gcccggaacc
     1561 gcacgctgct ggcacgcatg tgcagagaag accccaacta ctactcctac ctggagatgg
     1621 acctgcagtg ccgggacccc gacatccacg ccgctgcctt tggcacctgc ctggccgcct
     1681 ccgtggctgc gcctggctct ggcagggtgc tatatgctgt cttcagcaga gacagccgga
     1741 gcagtggggg gcccggtgcg ggcctctgcc tgttcccgct ggacaaggtg cacgccaaga
     1801 tggaggccaa ccgcaacgcc tgttacacag gcacccggga ggcccgtgac atcttctaca
     1861 agcccttcca cggcgatatc cagtgcggcg gccacgcgcc gggctccagc aagagcttcc
     1921 catgtggctc ggagcacctg ccctacccgc tgggcagccg cgacgggctc agaggcacag
     1981 ccgtgctgca gcgtggaggc ctgaacctca cggctgtcac cgtggctgct gaaaataacc
     2041 acactgttgc ttttctgggc acctctgatg gccggatcct caaggtgtac ctcaccccag
     2101 atggcacctc ctcagagtac gactctatcc ttgtggagtg taacaagaga gtcaagcgcg
     2161 acctggtact gtctggagac ctgggcagcc tgtacgccat gacccaggac aaggtgttcc
     2221 ggctgccggt gcaggagtgc ctgagctacc cgacctgcac ccagtgccgc gactcccagg
     2281 acccctactg cggctggtgc gtcgtcgagg gacgatgcac ccggaaggcc gagtgtccgc
     2341 gggccgagga ggccagccac tggctgtgga gccgaagcaa gtcctgcgtg gccgtcacca
     2401 gcgcccagcc acagaacatg agccggcggg cccaggggga ggtgcagctg accgtcagcc
     2461 ccctccctgc cctgagcgag gaggacgagt tgctgtgcct ttttggggag tcgccgccac
     2521 accccgcccg cgtggagggc gaggccgtca tctgcaactc cccaagcagc atccccgtca
     2581 caccgccagg ccaggaccac gtggccgtga ccatccagct cctccttaga cgaggcaaca
     2641 tcttcctcac gtcctaccag taccccttct acgactgccg ccaggccatg agcctggagg
     2701 agaacctgcc gtgcatctcc tgcgtgagca accgctggac ctgccagtgg gacctgcgct
     2761 accacgagtg ccgggaggct tcgcccaacc ctgaggacgg catcgtccgt gcccacatgg
     2821 aggacagctg tccccagttc ctgggaccca gccccctggt gatccccatg aaccacgaga
     2881 cagatgtgaa cttccagggc aagaacctgg acaccgtgaa gggttcctcc ctgcacgtgg
     2941 gcagtgactt gctcaagttc atggagccgg tgaccatgca ggaatctggg accttcgcct
     3001 ttcggacccc aaagctgtcc cacgatgcca acgagacgct gcccctgcac ctctacgtca
     3061 agtcttacgg caagaatatc gacagcaagc tccatgtgac cctctacaac tgctcctttg
     3121 gccgcagcga ctgcagcctg tgccgggccg ctaaccccga ctacaggtgt gcgtggtgcg
     3181 ggggccagag caggtgcgtg tatgaggccc tgtgcaacac cacctccgag tgcccgccgc
     3241 ccgtcatcac caggatccag cctgagacgg gccccctggg tgggggcatc cgcatcacca
     3301 tcctggggtc caatttgggc gtccaagcag gggacatcca gaggatctct gtggccggcc
     3361 ggaactgctc ctttcagccg gaacgttact ccgtgtccac ccggatcgtg tgtgtgatcg
     3421 aggctgcgga gacgcctttc acggggggtg tcgaggtgga cgtcttcggg aaactgggcc
     3481 gttcgcctcc caatgtccag ttcaccttcc aacagcccaa gcctctcagt gtggagccgc
     3541 agcagggacc gcaggcgggc ggcaccacac tgaccatcca cggcacccac ctggacacgg
     3601 gctcccagga ggacgtgcgg gtgaccctca acggcgtccc gtgtaaagtg acgaagtttg
     3661 gggcgcagct ccagtgtgtc actggccccc aggcgacacg gggccagatg cttctggagg
     3721 tctcctacgg ggggtccccc gtgcccaacc ccggcatctt cttcacctac cgcgaaaacc
     3781 ccgtactgcg agccttcgag ccgctacgaa gctttgccag tggtggccgc agcatcaacg
     3841 tcacgggtca gggcttcagc ctgatccaga ggtttgccat ggtggtcatc gcggagcccc
     3901 tgcagtcctg gcagccgccg cgggaggctg aatccctgca gcccatgacg gtggtgggta
     3961 cagactacgt gttccacaat gactgcaagg tcgtcttcct gtccccggct gtgcctgagg
     4021 agccagaggc ctacaacctc acggtgctga tcgagatgga cgggcaccgt gccctgctca
     4081 gaacagaggc cggggccttc gagtacgtgc ctgaccccac ctttgagaac ttcacaggtg
     4141 gcgtcaagaa gcaggtcaac aagctcatcc acgcccgggg caccaatctg aacaaggcga
     4201 tgacgctgca ggaggccgag gccttcgtgg gtgccgagcg ctgcaccatg aagacgctga
     4261 cggagaccga cctgtactgt gagcccccgg aggtgcagcc cccgcccaag cggcggcaga
     4321 aacgagacac cacacacaac ctgcccgagt tcattgtgaa gttcggctct cgcgagtggg
     4381 tgctgggccg cgtggagtac gacacacggg tgagcgacgt gccgctcagc ctcatcttgc
     4441 cgctggtcat cgtgcccatg gtggtcgtca tcgcggtgtc tgtctactgc tactggagga
     4501 agagccagca ggccgaacga gagtatgaga agatcaagtc ccagctggag ggcctggagg
     4561 agagcgtgcg ggaccgctgc aagaaggaat tcacagacct gatgatcgag atggaggacc
     4621 agaccaacga cgtgcacgag gccggcatcc ccgtgctgga ctacaagacc tacaccgacc
     4681 gcgtcttctt cctgccctcc aaggacggcg acaaggacgt gatgatcacc ggcaagctgg
     4741 acatccctga gccgcggcgg ccggtggtgg agcaggccct ctaccagttc tccaacctgc
     4801 tgaacagcaa gtctttcctc atcaatttca tccacaccct ggagaaccag cgggagttct
     4861 cggcccgcgc caaggtctac ttcgcgtccc tgctgacggt ggcgctgcac gggaaactgg
     4921 agtactacac ggacatcatg cacacgctct tcctggagct cctggagcag tacgtggtgg
     4981 ccaagaaccc caagctgatg ctgcgcaggt ctgagactgt ggtggagagg atgctgtcca
     5041 actggatgtc catctgcctg taccagtacc tcaaggacag tgccggggag cccctgtaca
     5101 agctcttcaa ggccatcaaa catcaggtgg aaaagggccc ggtggatgcg gtacagaaga
     5161 aggccaagta cactctcaac gacacggggc tgctggggga tgatgtggag tacgcacccc
     5221 tgacggtgag cgtgatcgtg caggacgagg gagtggacgc catcccggtg aaggtcctca
     5281 actgtgacac catctcccag gtcaaggaga agatcattga ccaggtgtac cgtgggcagc
     5341 cctgctcctg ctggcccagg ccagacagcg tggtcctgga gtggcgtccg ggctccacag
     5401 cgcagatcct gtcggacctg gacctgacgt cacagcggga gggccggtgg aagcgcgtca
     5461 acacccttat gcactacaat gtccgggatg gagccaccct catcctgtcc aaggtggggg
     5521 tctcccagca gccggaggac agccagcagg acctgcctgg ggagcgccat gccctcctgg
     5581 aggaggagaa ccgggtgtgg cacctggtgc ggccgaccga cgaggtggac gagggcaagt
     5641 ccaagagagg cagcgtgaaa gagaaggagc ggacgaaggc catcaccgag atctacctga
     5701 cgcggctgct ctcagtcaag ggcacactgc agcagtttgt ggacaacttc ttccagagcg
     5761 tgctggcgcc tgggcacgcg gtgccacctg cagtcaagta cttcttcgac ttcctggacg
     5821 agcaggcaga gaagcacaac atccaggatg aagacaccat ccacatctgg aagacgaaca
     5881 gcttaccgct ccggttctgg gtgaacatcc tcaagaaccc ccacttcatc tttgacgtgc
     5941 atgtccacga ggtggtggac gcctcgctgt cagtcatcgc gcagaccttc atggatgcct
     6001 gcacgcgcac ggagcataag ctgagccgcg attctcccag caacaagctg ctgtacgcca
     6061 aggagatctc cacctacaag aagatggtgg aggattacta caaggggatc cggcagatgg
     6121 tgcaggtcag cgaccaggac atgaacacac acctggcaga gatttcccgg gcgcacacgg
     6181 actccttgaa caccctcgtg gcactccacc agctctacca atacacgcag aagtactatg
     6241 acgagatcat caatgccttg gaggaggatc ctgccgccca gaagatgcag ctggccttcc
     6301 gcctgcagca gattgccgct gcactggaga acaaggtcac tgacctctag ggcctcatgg
     6361 gcccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgacaatca
     6421 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6481 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     6541 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     6601 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     6661 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     6721 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     6781 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     6841 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     6901 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     6961 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctgcagat
     7021 atccggacta gtgatctaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     7081 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     7141 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     7201 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     7261 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     7321 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     7381 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     7441 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     7501 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagccaata
     7561 tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7621 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7681 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7741 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7801 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7861 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7921 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7981 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     8041 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     8101 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     8161 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     8221 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     8281 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     8341 gccttcttga cgagttcttc tgaggggatc cgtcgagggc ccggtacctt taagaccaat
     8401 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     8461 gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     8521 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8581 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8641 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     8701 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     8761 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8821 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8881 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     8941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9001 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     9061 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     9121 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     9181 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     9241 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9301 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9361 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9421 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9481 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9541 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9601 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9661 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     9721 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9841 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9901 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9961 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    10021 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    10081 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    10141 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    10201 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    10261 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    10321 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    10381 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    10441 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    10501 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    10561 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    10621 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    10681 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    10741 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10801 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10861 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10921 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10981 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    11041 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    11101 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    11161 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    11221 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    11281 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    11341 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    11401 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    11461 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    11521 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    11581 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    11641 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    11701 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    11761 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    11821 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
    11881 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
    11941 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
    12001 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
    12061 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
    12121 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
    12181 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    12241 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    12301 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    12361 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    12421 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    12481 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    12541 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    12601 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    12661 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    12721 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    12781 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    12841 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    12901 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    12961 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    13021 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    13081 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    13141 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    13201 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    13261 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    13321 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    13381 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    13441 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    13501 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    13561 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    13621 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga agg
//
