LOCUS       sequence-360146-        9307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-CYLD
FEATURES             Location/Qualifiers
     source          1..9307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1418..1427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1427..1450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1463..1489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1505..1529
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4409..4433)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4446..5013
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4463..5015
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4607..4624)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4834..4853
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5027..5626
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5027..5046)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5523..5543
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5702..6216
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6378..6394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6402..6408,6409..6426,6427..6432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6447..6468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6585..6602)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6756..7344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6836..6855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7515..8306,8307..8375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8138..8157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8376..8480)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8548..8566
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8604..8626)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8726..8745
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8939..8961
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9089..9108
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctgcc accatggact acaaggatga
     1441 cgatgacaag ctcgatggag gatacccata cgatgttcca gattacgctg ctcgaggtta
     1501 tcaaacaagt ttgtacaaaa aagttggcgc gcccaccatg agttcaggct tatggagcca
     1561 agaaaaagtc acttcaccct actgggaaga gcggattttt tacttgcttc ttcaagaatg
     1621 cagcgttaca gacaaacaaa cacaaaagct ccttaaagta ccgaagggaa gtataggaca
     1681 gtatattcaa gatcgttctg tggggcattc aaggattcct tctgcaaaag gcaagaaaaa
     1741 tcagattgga ttaaaaattc tagagcaacc tcatgcagtt ctctttgttg atgaaaagga
     1801 tgttgtagag ataaatgaaa agttcacaga gttacttttg gcaattacca attgtgagga
     1861 gaggttcagc ctgtttaaaa acagaaacag actaagtaaa ggcctccaaa tagacgtggg
     1921 ctgtcctgtg aaagtacagc tgagatctgg ggaagaaaaa tttcctggag ttgtacgctt
     1981 cagaggaccc ctgttagcag agaggacagt ctccggaata ttctttggag ttgaattgct
     2041 ggaagaaggt cgtggtcaag gtttcactga cggggtgtac caagggaaac agctttttca
     2101 gtgtgatgaa gattgtggcg tgtttgttgc attggacaag ctagaactca tagaagatga
     2161 tgacactgca ttggaaagtg attacgcagg tcctggggac acaatgcagg tcgaacttcc
     2221 tcctttggaa ataaactcca gagtttcttt gaaggttgga gaaacaatag aatctggaac
     2281 agttatattc tgtgatgttt tgccaggaaa agaaagctta ggatattttg ttggtgtgga
     2341 catggataac cctattggca actgggatgg aagatttgat ggagtgcagc tttgtagttt
     2401 tgcgtgtgtt gaaagtacaa ttctattgca catcaatgat atcatcccag agagtgtgac
     2461 gcaggaaagg aggcctccca aacttgcctt tatgtcaaga ggtgttgggg acaaaggttc
     2521 atccagtcat aataaaccaa aggctacagg atctacctca gaccctggaa atagaaacag
     2581 atctgaatta ttttatacct taaatgggtc ttctgttgac tcacaaccac aatccaaatc
     2641 aaaaaataca tggtacattg atgaagttgc agaagaccct gcaaaatctc ttacagagat
     2701 atctacagac tttgaccgtt cttcaccacc actccagcct cctcctgtga actcactgac
     2761 caccgagaac agattccact ctttaccatt cagtctcacc aagatgccca ataccaatgg
     2821 aagtattggc cacagtccac tttctctgtc agcccagtct gtaatggaag agctaaacac
     2881 tgcacccgtc caagagagtc cacccttggc catgcctcct gggaactcac atggtctaga
     2941 agtgggctca ttggctgaag ttaaggagaa ccctcctttc tatggggtaa tccgttggat
     3001 cggtcagcca ccaggactga atgaagtgct cgctggactg gaactggaag atgagtgtgc
     3061 aggctgtacg gatggaacct tcagaggcac tcggtatttc acctgtgccc tgaagaaggc
     3121 gctgtttgtg aaactgaaga gctgcaggcc tgactctagg tttgcatcat tgcagccggt
     3181 ttccaatcag attgagcgct gtaactcttt agcatttgga ggctacttaa gtgaagtagt
     3241 agaagaaaat actccaccaa aaatggaaaa agaaggcttg gagataatga ttgggaagaa
     3301 gaaaggcatc cagggtcatt acaattcttg ttacttagac tcaaccttat tctgcttatt
     3361 tgcttttagt tctgttctgg acactgtgtt acttagaccc aaagaaaaga acgatgtaga
     3421 atattatagt gaaacccaag agctactgag gacagaaatt gttaatcctc tgagaatata
     3481 tggatatgtg tgtgccacaa aaattatgaa actgaggaaa atacttgaaa aggtggaggc
     3541 tgcatcagga tttacctctg aagaaaaaga tcctgaggaa ttcttgaata ttctgtttca
     3601 tcatatttta agggtagaac ctttgctaaa aataagatca gcaggtcaaa aggtacaaga
     3661 ttgttacttc tatcaaattt ttatggaaaa aaatgagaaa gttggcgttc ccacaattca
     3721 gcagttgtta gaatggtctt ttatcaacag taacctgaaa tttgcagagg caccatcatg
     3781 tctgattatt cagatgcctc gatttggaaa agactttaaa ctatttaaaa aaatttttcc
     3841 ttctctggaa ttaaatataa cagatttact tgaagacact cccagacagt gccggatatg
     3901 tggagggctt gcaatgtatg agtgtagaga atgctacgac gatccggaca tctcagctgg
     3961 aaaaatcaag cagttttgta aaacctgcaa cactcaagtc caccttcatc cgaagaggct
     4021 gaatcataaa tataacccag tgtcacttcc caaagactta cccgactggg actggagaca
     4081 cggctgcatc ccttgccaga atatggagtt atttgctgtt ctctgcatag aaacaagcca
     4141 ctatgttgct tttgtgaagt atgggaagga cgattctgcc tggctcttct ttgacagcat
     4201 ggccgatcgg gatggtggtc agaatggctt caacattcct caagtcaccc catgcccaga
     4261 agtaggagag tacttgaaga tgtctctgga agacctgcat tccttggact ccaggagaat
     4321 ccaaggctgt gcacgaagac tgctttgtga tgcatatatg tgcatgtacc agagtccaac
     4381 aatgagtttg tacaaataag gccggcctac ccaactttct tgtacaaagt ggttcgataa
     4441 cgaattccgc cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4501 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4561 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4621 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4681 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4741 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     4801 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     4861 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     4921 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     4981 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga ccgagtacaa
     5041 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5101 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5161 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5221 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5281 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     5341 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     5401 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     5461 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     5521 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     5581 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgatgca tcgatgtcga
     5641 cctgcagcca agcttatcga taaaataaaa gattttattt agtctccaga aaaagggggg
     5701 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     5761 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
     5821 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     5881 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
     5941 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     6001 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     6061 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa
     6121 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     6181 tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct tgaagttgga
     6241 gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat tagccactgt
     6301 tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg cagaaagagc
     6361 tggggagaat tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7501 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7561 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7621 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7681 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7741 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7801 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7861 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7921 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7981 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8041 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8101 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8161 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8221 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8281 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8341 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8401 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8461 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8521 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     8581 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     8641 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     8701 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     8761 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     8821 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8881 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     8941 cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc atgcaaggag atggcgccca
     9001 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     9061 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     9121 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggcga ttagtccaat
     9181 ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc
     9241 tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa
     9301 agggggg
//
