LOCUS       sequence-360152-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning 
            ToolKit..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Mozsik L, Pohl C, Meyer V, Bovenberg RAL, Nygard Y, Driessen AJM
  TITLE     Modular Synthetic Biology Toolkit for Filamentous Fungi.
  JOURNAL   ACS Synth Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260.
  PUBMED    34726388
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Nov 2. doi: 10.1021/acssynbio.1c00260."
COMMENT     Sequence Label: pFTK019
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(71..89,90..112,113..131,132..154,155..173,174..196,
                     197..215,216..238,239..257,258..280,281..299,300..322,
                     323..341)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(113..131,132..154,155..173,174..196,197..215,216..238,
                     239..257,258..280,281..299,300..322,323..341,342..364,
                     365..383)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(155..173,174..196,197..215,216..238,239..257,258..280,
                     281..299,300..322,323..341,342..364,365..383,384..406,
                     407..425)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(197..215,216..238,239..257,258..280,281..299,300..322,
                     323..341,342..364,365..383,384..406,407..425,426..448,
                     449..467)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(239..257,258..280,281..299,300..322,323..341,342..364,
                     365..383,384..406,407..425,426..448,449..467,468..490,
                     491..509)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          579..694
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     primer_bind     complement(742..760)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgcgttggc cgattcatta atcactctgt ggtctcagga ggccctttcg tattcactcg
       61 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      121 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
      181 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
      241 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
      301 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      361 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      421 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      481 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagctcccc atcttcagta
      541 tattcatctt cccatccaag aacctttatt tcccctaagt aagtactttg ctacatccat
      601 actccatcct tcccatccct tattcctttg aacctttcag ttcgagcttt cccacttcat
      661 cgcagcttga ctaacagcta ccccgcttga gcagacatca ccaatgtgag accacgaagt
      721 ggctcttcag tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      781 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      841 tttgtttatt tttctaaata c
//
