LOCUS       sequence-360165-       10498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHR-CAG-sfGFP* ABE_3-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Kempton HR, Love KS, Guo LY, Qi LS
  TITLE     Scalable biological signal recording in mammalian cells using Cas12a
            base editors.
  JOURNAL   Nat Chem Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2.
  PUBMED    35637351
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2022 May 30. pii: 10.1038/s41589-022-01034-2. doi: 
            10.1038/s41589-022-01034-2."
COMMENT     Sequence Label: pSLQ11353
FEATURES             Location/Qualifiers
     source          1..10498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             13..246
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(271..289)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(272..289)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(579..598)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(812..831)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     931..953
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(991..1009)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1077..1181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1182..1250,1251..2042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1400..1419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2213..2801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2702..2721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2955..2972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3042..3062)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3047..3376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3227..3362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4510..4575
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4705..4725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5150..5284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5187..5206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5241..5260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5452..6085
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6132..6257
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6754..6987
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7172..7216
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7501..7618
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7686..7962
                     /label=chicken beta-actin promoter
     intron          7963..8979
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     8903..8925
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     8987..9006
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             9037..9750
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVN*IELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(9067..9095)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     9690..9710
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    9813..10401
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9866..9886)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10284..10295)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10404..10420)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10405..10421)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
       61 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      121 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      181 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      241 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
      301 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
      361 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
      421 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
      481 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
      541 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
      601 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
      661 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
      721 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
      781 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
      841 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
      901 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
      961 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     1021 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1081 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1141 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1201 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1261 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1321 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1381 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1441 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     1501 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     1561 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1621 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1681 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1741 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1801 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1861 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1921 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1981 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2041 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2101 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2161 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2221 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2281 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2341 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2401 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2461 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2521 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2581 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2641 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2701 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2761 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2821 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2881 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2941 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3001 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     3061 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3121 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3181 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3241 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3301 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3361 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     3421 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     3481 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     3541 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     3601 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     3661 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     3721 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     3781 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     3841 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     3901 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     3961 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     4021 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     4081 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     4141 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     4201 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     4261 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     4321 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     4381 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     4441 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     4501 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     4561 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     4621 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     4681 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     4741 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     4801 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     4861 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     4921 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     4981 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     5041 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5101 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5281 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     5341 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     5401 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     5461 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     5521 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     5581 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     5641 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     5701 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     5761 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     5821 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     5881 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     5941 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6001 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6061 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     6121 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     6181 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     6241 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     6301 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     6361 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     6421 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     6481 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     6541 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     6601 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     6661 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     6721 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     6781 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     6841 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     6901 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     6961 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     7021 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     7081 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     7141 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7201 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     7261 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     7321 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     7381 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     7441 atccattcga ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa
     7501 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     7561 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     7621 ggtttattac agggacagca gagatccagt ttggatcgat aagcttgata tcgaattccc
     7681 atgggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     7741 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     7801 gggggncgcg cgccaggcgg ggcggggcgg ggcgrggggc ggggcggggc gaggcggaga
     7861 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     7921 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
     7981 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     8041 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
     8101 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
     8161 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
     8221 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
     8281 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
     8341 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
     8401 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     8461 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     8521 ccgggcgggg ggtggcggca ggkgggggtg ccgggcgggg cggggccgcc tcgggccggg
     8581 grgggctcgg gggrggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
     8641 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
     8701 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     8761 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     8821 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     8881 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     8941 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     9001 attgtgctgt ctcatcattt tggcaaaacg cgtaccatga gcaaaggaga agaacttttc
     9061 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     9121 gtccgtggag agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc
     9181 actactggaa aactacctgt tccgtggcca acacttgtca ctactctgac ctatggtgtt
     9241 caatgctttt cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg
     9301 cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg
     9361 cgtgctgaag tcaagtttga aggtgatacc cttgttaact gaatcgagtt aaagggtatt
     9421 gattttaaag aagatggaaa cattcttgga cacaaactcg agtacaactt taactcacac
     9481 aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc
     9541 cacaacgttg aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     9601 ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg
     9661 aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg
     9721 attacacatg gcatggatga gctctacaaa taagcggccg cgactctaga gtcgacctgc
     9781 aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     9841 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     9901 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     9961 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
    10021 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
    10081 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
    10141 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
    10201 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
    10261 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
    10321 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
    10381 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg
    10441 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaag
//
