LOCUS       sequence-360166-       12854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GluN2A in pCDNA3.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12854)
  AUTHORS   Cappelli J, Khacho P, Wang B, Sokolovski A, Bakkar W, Raymond S, 
            Ahlskog N, Pitney J, Wu J, Chudalayandi P, Wong AYC, Bergeron R
  TITLE     Glycine-induced NMDA receptor internalization provides 
            neuroprotection and preserves vasculature following ischemic stroke.
  JOURNAL   iScience. 2021 Dec 3;25(1):103539. doi: 10.1016/j.isci.2021.103539. 
            eCollection 2022 Jan 21.
  PUBMED    34977503
REFERENCE   2  (bases 1 to 12854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2021-12-3"; volume: "25(1):103539. doi"; pages: " 
            10.1016/j.isci.2021.103539. eCollection 2022 Jan 21"
COMMENT     Sequence Label: pRB133
FEATURES             Location/Qualifiers
     source          1..12854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        87..466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        467..670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     621..641
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     715..734
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        715..733
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8899..8917)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8900..8917)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          9001..9066
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9196..9216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9488..9622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9525..9544)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9579..9598
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10473..10608
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11103..11122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(11235..11693)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11372..11393)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11587..11606
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(11817..11835)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11903..12007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12008..12076,12077..12854,1..14)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12226..12245)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
       61 ttcattttta atttctctag cgcgttgaca ttgattattg actagttatt aatagtaatc
      121 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      181 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      241 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      301 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      361 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      421 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      481 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      541 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      601 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      661 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      721 gactcactat agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc
      781 agtgtgctgg aattccgttg ctgtcgcagg agcgctcaga ggaccggcag tcgctgtccg
      841 gagtggaaca gaaagctgag ccagggctct agaagagagg ctcctgaggt gctgtgcctg
      901 agcatggggc tggatgaggt ctgagagtcg cggcggcagc aatcagccct ggagatgacc
      961 aggggtggcc actgctgaga actatgtgga gagaggctgc gagccctgct gcagagcctc
     1021 cggctgggat agccgccccc cgtgggggtg atgcggacag cgcgggacag ccaggggagc
     1081 gcgcgggggc cgcagcatgc gggaacccgc taaacctggt ggctgctgag gcggccgaga
     1141 tgctcgtgcg cgcagcacgc cccattgcat cctccacctt ctccggctac agggacccta
     1201 agtggcgacc atgggcagat tgggctactg gaccttgctg gtattgccgg cccttctggt
     1261 ctggcgcgat ccggcgcaga acgcggcggc ggagaagggt cctccagcgc tgaacattgc
     1321 ggtgctgctg ggtcacagcc acgacgtgac agaacgcgaa cttcgaaatc tgtggggccc
     1381 agagcaggca accggcttgc ccctggatgt gaacgtggtg gcgttattga tgaaccgcac
     1441 tgaccctaag agcctcatca cgcatgtgtg cgacctcatg tccggggcgc gcatccacgg
     1501 cttggtgttt ggagatgaca cggaccagga ggctgtggcc cagatgctgg attttatctc
     1561 ctcacagact tttatcccca tcttgggcat tcatgggggt gcatctatga tcatggctga
     1621 caaggatccg acatccacgt tcttccagtt tggagcctcc atccagcagc aagccacagt
     1681 tatgctgaag atcatgcagg actacgactg gcacgtcttc tccctggtca ccaccatctt
     1741 ccctggctac cgagacttca tcagctttat caagacaaca gtggacaaca gctttgtggg
     1801 ctgggatatg cagaacgtga tcacactgga cacctccttc gaggacgcca agacgcaggt
     1861 ccagctgaag aagatccatt cttctgtcat cctgctctac tgctccaaag acgaggctgt
     1921 cctcatcctg agcgaggctc gctccttcgg cctcactggc tatgatttct tctggattgt
     1981 ccccagtttg gtgtctggga acacagagct catccccaaa gagtttccat caggtctcat
     2041 ttcagtctct tatgacgact gggactacag cctggaggca agagtgagag acggtcttgg
     2101 gatcttaacc actgccgcat cctccatgtt ggagaagttc tcctacattc ctgaggccaa
     2161 ggccagctgc tatgggcagg cagagaagcc agagaccccg ctacacaccc tgcaccaatt
     2221 catggtcaat gtgacttggg atggcaagga cttgtccttc actgaggaag gttaccaggt
     2281 gcaccccagg cttgtggtga tcgtgctgaa caaggaccgg gagtgggaaa aggtgggcaa
     2341 gtgggagaac cagacgctga gcctgaggca cgctgtgtgg ccaaggtaca agtccttttc
     2401 tgactgcgag ccagatgaca accacctcag cattgtcacc ttggaggaag cccccttcgt
     2461 catcgtagag gacatagacc ccctgactga gacctgtgtg aggaacacgg tgccctgtcg
     2521 gaagttcgtc aagatcaaca attcaaccaa cgaagggatg aatgtgaaga aatgctgcaa
     2581 ggggttctgc atcgacatcc tcaagaagct gtccagaact gtgaagttca cctatgacct
     2641 ctacctggtg accaatggga agcatgggaa aaaggttaac aatgtgtgga atggaatgat
     2701 aggtgaagtg gtctatcaac gagcagtcat ggctgtgggc tcactcacca tcaatgagga
     2761 gcgttcggaa gtggtggact tctcggtgcc ctttgtggag acaggaatca gcgtcatggt
     2821 ctccaggagt aatggcactg tctccccttc tgctttcctc gaacccttca gtgcctccgt
     2881 ctgggtgatg atgtttgtga tgctgctcat cgtctcagcc attgctgtct tcgtttttga
     2941 atacttcagt cctgttggat acaacagaaa cttagccaaa gggaaagctc cccacgggcc
     3001 ttcttttact attggaaaag ctatatggct cctctggggc ctggtcttca acaattctgt
     3061 gcctgtccag aatcctaaag gcacaaccag caagatcatg gtgtcagtgt gggccttctt
     3121 tgctgtcatc ttcctggcca gttacacagc caacctggct gccttcatga tccaggagga
     3181 gtttgtggac caagtgactg gcctcagtga caagaagttc cagagacctc atgactattc
     3241 tccacctttc cgatttggga cggtacccaa tggaagtaca gagaggaata ttcgtaacaa
     3301 ctacccgtat atgcaccagt acatgaccag attcaaccag aggggagtgg aggatgcctt
     3361 ggtcagcttg aaaaccggga agttggacgc tttcatctat gacgcagccg tcttgaacta
     3421 caaggccggg agggatgaag gctgtaaact ggtgaccatt gggagcgggt acatctttgc
     3481 taccacaggc tatggaattg cgctgcagaa gggctcaccc tggaagaggc agattgacct
     3541 cgctctgctc cagtttgttg gtgatggtga gatggaggag ctggagacac tgtggcttac
     3601 gggcatctgc cacaacgaga agaatgaggt gatgagtagc cagctggaca tcgataacat
     3661 ggcgggcgtg ttctacatgc tggctgcagc catggccctc agcctcatca ccttcatctg
     3721 ggagcacctc ttctactgga agctgcgctt ctgcttcaca ggcgtgtgct ctgaccggcc
     3781 cgggctgctc ttctccatca gcaggggcat ctatagttgc atccatgggg tacacattga
     3841 agaaaagaag aaatctccag atttcaatct gactggatca cagagcaaca tgctaaagct
     3901 tcttcggtca gctaaaaaca tctccaatat gtccaacatg aactcctcaa gaatggactc
     3961 acctaaaaga gctactgact tcattcaaag agggtcactt attgtggaca tggtttcaga
     4021 caagggaaat ttgatatatt cagacaacag atcctttcaa gggaaggaca gtatatttgg
     4081 agacaacatg aatgaactcc aaacatttgt ggccaacagg cacaaggata atctcagtaa
     4141 ctatgtgttt caaggacagc atcctctcac tctcaatgag tccaacccta acacagtaga
     4201 ggtggctgtc agcactgaat ccaaagggaa ctcccgaccc cggcagcttt ggaagaaatc
     4261 catggagtct ctacgccagg attctctaaa ccagaaccca gtctcccaga gggatgagaa
     4321 gactgcagag aatcggaccc actcgctaaa gagtcctagg tatcttccag aagaggtagc
     4381 ccactctgac atttcagaaa cctcaagccg ggccacatgc cacagggagc cagataacaa
     4441 taagaaccac aagaccaagg ataacttcaa acggtcaatg gcctctaagt atcccaagga
     4501 ctgtagcgat gttgaccgca cctacatgaa aaccaaagca agttctccca gggataagat
     4561 ctataccatt gatggtgaga aggagcccag cttccactta gatcctcctc agtttgttga
     4621 gaatataacc ctgcctgaga atgtgggctt cccagatacc taccaagatc acaatgagaa
     4681 cttccgcaag ggggactcca cactgcccat gaacaggaac ccattacata atgaagacgg
     4741 gcttcccaac aatgaccaat ataaactcta tgccaagcac tttaccttga aagacaaggg
     4801 ttccccacac agtgagggca gtgatcgata ccggcagaac tccacacatt gcagaagctg
     4861 cctttcgaat ctgcccacct actcaggcca ctttaccatg aggtctcctt tcaagtgtga
     4921 tgcctgtctg cggatgggga atctctatga cattgatgaa gaccagatgc ttcaggagac
     4981 aggtaaccca gctactcggg aggaggtcta ccagcaggac tggtcacaga acaacgccct
     5041 ccagttccag aagaacaagc taaggattaa ccgacagcac tcctatgata acattctgga
     5101 caaacccaga gagatagacc ttagcaggcc ctcccggagc ataagcctca aggacaggga
     5161 acggctactg gagggcaact tgtatgggag cctgttcagt gtcccctcaa gcaaactctt
     5221 ggggaacaaa agctcccttt tcccccaagg tctggaggac agcaagagga gcaagtctct
     5281 cttgccagac cacgcctccg ataatccttt cctccacacg tatggggatg accaacgctt
     5341 agttatcggg agatgtccct cggaccctta caaacactca ttgccatcac aggcggtaaa
     5401 tgacagctat cttcggtcat ccttgaggtc aacagcatca tattgctcca gggacagtcg
     5461 gggccacagt gatgtgtata tttcagagca tgttatgcct tatgctgcaa ataagaatac
     5521 catgtactct acccccaggg ttttaaattc ctgcagcaat agacgagtgt acaagaaaat
     5581 gcctagtatc gaatctgatg tttaagatct tccatcagta tttatctata aggaaacata
     5641 tagaatggcc aacattatag agggtaaatg ttggatgtcc gatagcaccc tactaggagg
     5701 aagagggtac agggaggtac tttttgttgg ctcttttgca catggctcca tgccataatc
     5761 ttccactcaa ggaatcttgt gagatatgtg ctgagcacag catataccac gtaggtgaat
     5821 ccttaaccaa aaacaaataa atacacatgg gcaagtctcc cagacatggc gactgggcac
     5881 ggcggcaata atggtgcatc agacggcgat ggtgacattg tggttgccta tattccaaat
     5941 tccatgaaga tcaatccacc atgtaacttc ccccatcaga aatgcctcac agtttctcta
     6001 atacagaata agcaatatgg tatgcatgta agtctgacgc agacaataag acagttaaga
     6061 atgcatctgc actgtagtaa gattgacatg tgcaaggatt aagaagtttg gcttgtaaca
     6121 gttctcagat ttcttgttat gccttccatg acaaccctgg atcagtcccc cttaaactcc
     6181 aggttcgccc cccccnccna aagaatagca aatcagcgtg tttgtggcac ccacactact
     6241 ttcattgtag gcttccccca agacacacct ggaacaaata gccccaggga ctctcaggtg
     6301 gagaactgta agaatctctt tggatgttaa tgtgtgtgta tctctcaccc tcagcttcat
     6361 tggtcttgtt cggagctgca taaacacttt tatgtcttcg atatctaagt gtggatcttc
     6421 tgtctatgac ataatggctg ttgtagttaa tcttgaagac tcatgtacgt aagtctgcat
     6481 ttcctccctt ctggtgatga ctcagggttg tatagtatct cttacctgac ccctcccaga
     6541 gtaacgtaac ttgttctgtc cccagacagc catgatggtg tctaactagc tgtgtgttgt
     6601 gttcctagag ttgttaatgt ggtgacatgt gccagcagcc atgtgtaatc tgtaagaagt
     6661 cccatgctat ctgtgtgtcc aaggctagca tggttttaca tttatgtccc tcaaagtcag
     6721 gaaactctta gacaattatt tctatacaaa cctcctcaaa tcaaaagcct tcagacatca
     6781 gccacttgtg tgtccccccc caagcaccct tccaactgtg gataaactca gtaaaacaag
     6841 ggatttctat cctctgaaat gtacaccttc ttggaggtga atttcttccc cagcaccaac
     6901 agaaaccaat gagaagtctt caacgcattt gtcatttctg agctgcctgc attgtcctat
     6961 tcttggccca ctgagcctgt gcatatatga cagaacagat actcattaga ttaaaaaaaa
     7021 gaaaaactgg agccaatgag gggaatattc gtgcatggaa aatgtcttag cacataattt
     7081 tttatcacat ttttaaaaag gactcaaaag gtagttatga taccttatta gacaaggaca
     7141 ttcacaaaag cagaaaataa tatgaattaa acattttata tgcttctttt ctagcaatta
     7201 gtatatttcc aatgcattat tgtccttgaa atagccaggc tgctgaccgt ggtatttcgc
     7261 aggagacttt agttggttgg gtaactctct cccatgaagg attctaacca tttcttgttt
     7321 ttcctcttac tgaatcttgg cttgggttct tggagaaagg gatcgcacga caggttgaaa
     7381 tttcatccct gttcttttct tcgtggtgtt ctattttata ggtgggattg tccaactaca
     7441 agttaagcag ggaaggttct cttagagctt ggggctgctt cagaaggagc tagcagaggg
     7501 aaagttcatt tgacctacag tgttgcagaa tgacaaaaag tgatgcaata caccggagga
     7561 ccagaggtgg agtggcagag ggggtgagga tctctcttcc tgtgctcttg cagaacacag
     7621 aaggaggctg gggtggagct agagggctga ggtcacagga ctagggtgga gccagcggtc
     7681 tggggcggag ctagaaggct gaggtcacag gactagggtg gagccagtgg tctggggcgg
     7741 agctagaagg ctggggcgga gctagaagtt gataagcttt aaagtcacgt tttattcctt
     7801 ggacagagcc atcctttcct tccccaagtc ccacagctgg ggaaatgtgc attagagaac
     7861 ctagaacagt ctccctgatt tttgatctta aatttgagct tcagtgtgtg tagtgtcctt
     7921 tcaccccttt tactttattt ccccatagga ttccacacct gtattcctcc tgctaggact
     7981 gctgttgtgt cctcacagaa ctgactctta agcctccctg gaatacagaa tcatgacttg
     8041 tcacaaatct gaggagcatt ctgctttccc ggattgtcct caaagcccag ggtgctgttg
     8101 aatgtcccac attttagtga gagtacaaat ggcatttata ctgcctcctc aaaaccctct
     8161 ctggaaaatt caggatcatt tctgctatta actctgtaca aaagaagcta aaatgaggct
     8221 tttggaagaa tcagtcacat aatgggaaag aatacgtttg ttttaacacc aagatattta
     8281 caaaaataag tcatggtagt taggggctgt gtggcaaaca tttgcttttc cctctcctct
     8341 aatttgctgc ctcttgcaca tgataacttc aaagaagaaa acgaggaggt cacgttccaa
     8401 gtgagaggta atcatgtggg cttgtttgaa ggaaagccaa gtgagatcca tccttttcaa
     8461 aactaacccc gagggtggca aatggcacct acggccggtg atgagtactg agcaagaggc
     8521 cttggcctgg gtatcactta gagggttccc acactaaacc caacctctga gtccttagac
     8581 ttacagatgc taagaagcca gtgtctctta actcaaaggc ctgatctttc cctggactaa
     8641 gtcagggcag ctgccctggg cagttgtctt ggcaattctt ttgttttcct cattttggaa
     8701 aggaccagac tggtcctcag atgtcaatac ttgaaagcaa acagccatgc atctgtcctt
     8761 gcacctgaac cggtgcaaat gctgtgcaca agagaagctc ccagagcatg accttcactc
     8821 ggccgcgcga cagcaacgga attctgcaga tatccatcac actggcggcc gctcgagcat
     8881 gcatctagag ggccctattc tatagtgtca cctaaatgct agaggatctt tgtgaaggaa
     8941 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     9001 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     9061 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     9121 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     9181 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     9241 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     9301 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatatttg
     9361 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     9421 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     9481 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9541 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9601 tgtatcttat catgtctgga tcgatcccgc catggtatca acgccatatt tctatttaca
     9661 gtagggacct cttcgttgtg taggtaccgc tgtattccta gggaaatagt agaggcacct
     9721 tgaactgtct gcatcagcca tatagccccc gctgttcgat ttacaaacac aggcacagta
     9781 ctgacaaacc catacacctc ctctgaaata cccatagttg ctagggctgt ctccgaactc
     9841 attacaccct ccaaagtcag agctgtaatt tcgccatcaa gggcagcgag ggcttctcca
     9901 gataaaatag cttctgccga gagtcccgta agggtagaca cttcagctaa tccctcgatg
     9961 aggtctacta gaatagtcag tgcggctccc attttgaaaa ttcacttact tgatcagctt
    10021 cagaagatgg cggagggcct ccaacacagt aattttcctc ccgactctta aaatagaaaa
    10081 tgtcaagtca gttaagcagg aagtgactaa ctgaccgcag ctggccgtgc gacatcctct
    10141 tttaattagt tgctaggcaa ctgccctcca gagggcagtg tggttttgca agaggaagca
    10201 aaaagcctct ccacccaggc ctagaatgtt tccacccaat cattactatg acaacagctg
    10261 ttttttttag tattaagcag aggccggggg cccctggcct ccgcttactc tggagaaaaa
    10321 gaagagaggc attgtagagg cttccagagg caacttgtca aaacaggact gcttctattt
    10381 ctgtcacact gtctggccct gtcacaaggt ccagcacctc cataccccct ttaataagca
    10441 gtttgggaac gggtgcgggt cttactccgc ccatcccgcc cctaactccg cccagttccg
    10501 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10561 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    10621 aaaagctaat tcggcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10681 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10741 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
    10801 tctgtagcac cgcctacata cctcgctctg ctgaagccag ttaccagtgg ctgctgccag
    10861 tggcgataag tcgtgtctta ccgggttgga ctcaagagat agttaccgga taaggcgcag
    10921 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10981 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    11041 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    11101 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    11161 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcaagc
    11221 tagcttctag ctagaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg
    11281 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
    11341 agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
    11401 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gccgcccact
    11461 acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    11521 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    11581 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    11641 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtactatgg
    11701 ttgctttgac gagcacgtat aacgtgcttt cctcgttaga atcagagcgg gagctaaaca
    11761 ggaggccgat taaagggatt ttagacagga acggtacgcc agctggaccc gaaagggcct
    11821 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
    11881 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    11941 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
    12001 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
    12061 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    12121 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    12181 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    12241 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    12301 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    12361 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    12421 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    12481 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    12541 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    12601 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    12661 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    12721 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    12781 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    12841 aggtgcctca ctga
//
