LOCUS       sequence-360170-        4480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses codon-optimized full length SARS-CoV-2 Delta Spike.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Benlarbi M, Laroche G, Fink C, Fu K, Mulloy RP, Phan A, Ariana A, 
            Stewart CM, Prevost J, Beaudoin-Bussieres G, Daniel R, Bo Y, El 
            Ferri O, Yockell-Lelievre J, Stanford WL, Giguere PM, Mubareka S, 
            Finzi A, Dekaban GA, Dikeakos JD, Cote M
  TITLE     Identification and differential usage of a host metalloproteinase 
            entry pathway by SARS-CoV-2 Delta and Omicron.
  JOURNAL   iScience. 2022 Nov 18;25(11):105316. doi: 
            10.1016/j.isci.2022.105316. Epub 2022 Oct 10.
  PUBMED    36254158
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-18"; volume: "25(11):105316. doi"; pages: " 
            10.1016/j.isci.2022.105316. Epub 2022 Oct 10"
COMMENT     Sequence Label: pCAGGS SARS-CoV-2 Delta Spike
FEATURES             Location/Qualifiers
     source          1..4480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3886..3905)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3951..4006
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4005..4024)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4367..4383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4367..4383)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4387..4410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4414..4420,4421..4438,4439..4444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4459..4480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gctcctgggc acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccacc
       61 atgttcgtct tcctcgtcct cctccccctc gtctcctccc agtgcgtcaa cctcagaacc
      121 cgcacccagc tcccccccgc ctacaccaac tccttcaccc gcggcgtcta ctaccccgac
      181 aaggtcttcc gctcctccgt cctccactcc acccaggacc tcttcctccc cttcttctcc
      241 aacgtcacct ggttccacgc catccacgtc tccggcacca acggcaccaa gcgcttcgac
      301 aaccccgtcc tccccttcaa cgacggcgtc tacttcgcct ccaccgagaa gtccaacatc
      361 atccgcggct ggatcttcgg caccaccctc gactccaaga cccagtccct cctcatcgtc
      421 aacaacgcca ccaacgtcgt catcaaggtc tgcgagttcc agttctgcaa cgaccccttc
      481 ctcggcgtct actaccacaa gaacaacaag tcctggatgg agtccggcgt ctactcctcc
      541 gccaacaact gcaccttcga gtacgtctcc cagcccttcc tcatggacct cgagggcaag
      601 cagggcaact tcaagaacct ccgcgagttc gtcttcaaga acatcgacgg ctacttcaag
      661 atctactcca agcacacccc catcaacctc gtccgcgacc tcccccaggg cttctccgcc
      721 ctcgagcccc tcgtcgacct ccccatcggc atcaacatca cccgcttcca gaccctcctc
      781 gccctccacc gctcctacct cacccccggc gactcctcct ccggctggac cgccggcgcc
      841 gccgcctact acgtcggcta cctccagccc cgcaccttcc tcctcaagta caacgagaac
      901 ggcaccatca ccgacgccgt cgactgcgcc ctcgaccccc tctccgagac caagtgcacc
      961 ctcaagtcct tcaccgtcga gaagggcatc taccagacct ccaacttccg cgtccagccc
     1021 accgagtcca tcgtccgctt ccccaacatc accaacctct gccccttcgg cgaggtcttc
     1081 aacgccaccc gcttcgcctc cgtctacgcc tggaaccgca agcgcatctc caactgcgtc
     1141 gccgactact ccgtcctcta caactccgcc tccttctcca ccttcaagtg ctacggcgtc
     1201 tcccccacca agctcaacga cctctgcttc accaacgtct acgccgactc cttcgtcatc
     1261 cgcggcgacg aggtccgcca gatcgccccc ggccagaccg gcaagatcgc cgactacaac
     1321 tacaagctcc ccgacgactt caccggctgc gtcatcgcct ggaactccaa caacctcgac
     1381 tccaaggtcg gcggcaacta caactaccgc taccgcctct tccgcaagtc caacctcaag
     1441 cccttcgagc gcgacatctc caccgagatc taccaggccg gctccaagcc ctgcaacggt
     1501 gtcgagggct tcaactgcta cttccccctc cagtcctacg gcttccagcc caccaacggc
     1561 gtcggctacc agccctaccg cgtcgtcgtc ctctccttcg agctcctcca cgcccccgcc
     1621 accgtctgcg gccccaagaa gtccaccaac ctcgtcaaga acaagtgcgt caacttcaac
     1681 ttcaacggcc tcaccggcac cggcgtcctc accgagtcca acaagaagtt cctccccttc
     1741 cagcagttcg gccgcgacat cgccgacacc accgacgccg tccgcgaccc ccagaccctc
     1801 gagatcctcg acatcacccc ctgctccttc ggcggcgtct ccgtcatcac ccccggcacc
     1861 aacacctcca accaggtcgc cgtcctctac cagggtgtca actgcaccga ggtccccgtc
     1921 gccatccacg ccgaccagct cacccccacc tggcgcgtct actccaccgg ctccaacgtc
     1981 ttccagaccc gcgccggctg cctcatcggc gccgagcacg tcaacaactc ctacgagtgc
     2041 gacatcccca tcggcgccgg catctgcgcc tcctaccaga cccagaccaa ctccagacgc
     2101 cgcgcccgct ccgtcgcctc ccagtccatc atcgcctaca ccatgtccct cggcgccgag
     2161 aactccgtcg cctactccaa caactccatc gccatcccca ccaacttcac catctccgtc
     2221 accaccgaga tcctccccgt ctccatgacc aagacctccg tcgactgcac catgtacatc
     2281 tgcggcgact ccaccgagtg ctccaacctc ctcctccagt acggctcctt ctgcacccag
     2341 ctcaaccgcg ccctcaccgg catcgccgtc gagcaggaca agaacaccca ggaggtcttc
     2401 gcccaggtca agcagatcta caagaccccc cccatcaagg acttcggcgg cttcaacttc
     2461 tcccagatcc tccccgaccc ctccaagccc tccaagcgct ccttcatcga ggacctcctc
     2521 ttcaacaagg tcaccctcgc cgacgccggc ttcatcaagc agtacggcga ctgcctcggc
     2581 gacatcgccg cccgcgacct catctgcgcc cagaagttca acggcctcac cgtcctcccc
     2641 cccctcctca ccgacgagat gatcgcccag tacacctccg ccctcctcgc cggcaccatc
     2701 acctccggct ggaccttcgg cgccggcgcc gccctccaga tccccttcgc catgcagatg
     2761 gcctaccgct tcaacggcat cggcgtcacc cagaacgtcc tctacgagaa ccagaagctc
     2821 atcgccaacc agttcaactc cgccatcggc aagatccagg actccctctc ctccaccgcc
     2881 tccgccctcg gcaagctcca gaacgtcgtc aaccagaacg cccaggccct caacaccctc
     2941 gtcaagcagc tctcctccaa cttcggcgcc atctcctccg tcctcaacga catcctctcc
     3001 cgcctcgaca aggtcgaggc cgaggtccag atcgaccgcc tcatcaccgg ccgcctccag
     3061 tccctccaga cctacgtcac ccagcagctc atccgcgccg ccgagatccg cgcctccgcc
     3121 aacctcgccg ccaccaagat gtccgagtgc gtcctcggcc agtccaagcg cgtcgacttc
     3181 tgcggcaagg gctaccacct catgtccttc ccccagtccg ccccccacgg cgtcgtcttc
     3241 ctccacgtca cctacgtccc cgcccaggag aagaacttca ccaccgcccc cgccatctgc
     3301 cacgacggca aggcccactt cccccgcgag ggcgtcttcg tctccaacgg cacccactgg
     3361 ttcgtcaccc agcgcaactt ctacgagccc cagatcatca ccaccgacaa caccttcgtc
     3421 tccggcaact gcgacgtcgt catcggcatc gtcaacaaca ccgtctacga ccccctccag
     3481 cccgagctcg actccttcaa ggaggagctc gacaagtact tcaagaacca cacctccccc
     3541 gacgtcgacc tcggcgacat ctccggcatc aacgcctccg tcgtcaacat ccagaaggag
     3601 atcgaccgcc tcaacgaggt cgccaagaac ctcaacgagt ccctcatcga cctccaggag
     3661 ctcggcaagt acgagcagta catcaagtgg ccctggtaca tctggctcgg cttcatcgcc
     3721 ggcctcatcg ccatcgtcat ggtcaccatc atgctctgct gcatgacctc ctgctgctcc
     3781 tgcctcaagg gctgctgctc ctgcggctcc tgctgcaagt tcgacgagga cgactccgag
     3841 cccgtcctca agggcgtcaa gctccactac acctaagcta gcagatcttt ttccctctgc
     3901 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     3961 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     4021 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     4081 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     4141 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     4201 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     4261 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     4321 cttatggaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc
     4381 ctgtgtgaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4441 taaagcctgg ggtgcctaat gagtgagcta actcacatta
//
