LOCUS       sequence-360203-       14046 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the rat TrkC fused in the N-terminus with mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14046)
  TITLE     Neurotrophin related plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLZRS-mVenus-TrkC
FEATURES             Location/Qualifiers
     source          1..14046
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             138..607
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(737..754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(908..1496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(988..1007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    1619..1753
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1643..1662)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1697..1716
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1983..2582)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2066..2086)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2563..2582
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2665..3164)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2713..2732)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3106..3129
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      5199..6988
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(7482..8273,8274..8342))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8105..8124
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8343..8447)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             8984..9453
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    9518..9875
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             9940..10356
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     10286..10308
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     10324..10340
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    10366..10740
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     10678..10694
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             join(10886..10888,10889..10891,10892..11602)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10931..10952)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11192..11211)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11539..11560
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
       61 gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
      121 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      181 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
      241 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
      301 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
      361 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
      421 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
      481 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
      541 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
      601 tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta
      661 aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      721 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     1021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     1081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     1141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1321 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1501 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1561 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgcgg ccgaattaga
     1621 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     1681 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     1741 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     1801 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     1861 tctagagtcg gtgggcctcg ggggcgggtg cggggtcggc ggggccgccc cgggtggctt
     1921 cggtcggagc catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
     1981 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     2041 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     2101 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     2161 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     2221 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     2281 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     2341 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     2401 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     2461 cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     2521 cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     2581 atggaaggtc gtctccttgt gaggggtcag gggcgtgggt caggggatgg tggcggcacc
     2641 ggtcgtggcg gccgacctgc aggtcgaaag gcccggagat gaggaagagg agaacagcgc
     2701 ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc
     2761 cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc ccagcctctg
     2821 agcccagaaa gcgaaggagc aaagctgcta ttggccgctg ccccaaaggc ctacccgctt
     2881 ccattgctca gcggtgctgt ccatctgcac gagactagtg agacgtgcta cttccatttg
     2941 tcacgtcctg cacgacgcga gctgcggggc gggggggaac ttcctgacta ggggaggagt
     3001 agaaggtggc gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt
     3061 ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg gggctgctaa
     3121 agcgcatgct ccagactgcc ttgggaaaag cgcctcccct acccggtaga attaattctc
     3181 atgtttgaca gcttatcatc gataagctga tcctcacagg ccgcacccag cttttcttcc
     3241 gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa
     3301 tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct
     3361 caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat
     3421 gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac
     3481 catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat
     3541 tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga
     3601 cgaggggcca ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc
     3661 agaaggctcc ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg
     3721 gggaagagga cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg
     3781 gccaagacat agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa
     3841 agggacccac ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc
     3901 aggagcagga ggaggggcag gagcaggagg nnnggcagga gcaggaggtg gaggccgggg
     3961 tcgaggaggc agtggaggcc ggggtcgagg aggtagtgga ggccggggtc gaggaggtag
     4021 tggaggccgc cggggtagag gacgtgaaag agccaggggg ggaagtcgtg aaagagccag
     4081 ggggagaggt cgtggacgtg gagaaaagag gcccaggagt cccagtagtc agtcatcatc
     4141 atccgggtct ccaccgcgca ggccccctcc aggtagaagg ccatttttcc accctgtagg
     4201 ggaagccgat tattttgaat accaccaaga aggtggccca gatggtgagc ctgacgtgcc
     4261 cccgggagcg atagagcagg gccccgcaga tgacccagga gaaggcccaa gcactggacc
     4321 ccggggtcag ggtgatggag gcaggcgcaa aaaaggaggg tggtttggaa agcatcgtgg
     4381 tcaaggaggt tccaacccga aatttgagaa cattgcagaa ggtttaagag ctctcctggc
     4441 taggagtcac gtagaaagga ctaccgacga aggaacttgg gtcgccggtg tgttcgtata
     4501 tggaggtagt aagacctccc tttacaacct aaggcgagga actgcccttg ctattccaca
     4561 atgtcgtctt acaccattga gtcgtctccc ctttggaatg gcccctggac ccggcccaca
     4621 acctggcccg ctaagggagt ccattgtctg ttatttcatg gtctttttac aaactcatat
     4681 atttgctgag gttttgaagg atgcgattaa ggaccttgtt atgacaaagc ccgctcctac
     4741 ctgcaatatc agggtgactg tgtgcagctt tgacgatgga gtagatttgc ctccctggtt
     4801 tccacctatg gtggaagggg ctgccgcgga gggtgatgac ggagatgacg gagatgaagg
     4861 aggtgatgga gatgagggtg aggaagggca ggagtgatgt aacttgttag gagacgccct
     4921 caatcgtatt aaaagccgtg tattcccccg cactaaagaa taaatcccca gtagacatca
     4981 tgcgtgctgt tggtgtattt ctggccatct gtcttgtcac cattttcgtc ctcccaacat
     5041 ggggcaattg ggcataccca tgttgtcacg tcactcagct ccgcgctcaa caccttctcg
     5101 cgttggaaaa cattagcgac atttacctgg tgagcaatca gacatgcgac ggctttagcc
     5161 tggcctcctt aaattcacct aagaatggga gcaaccagca ggaaaaggac aagcagcgaa
     5221 aattcacgcc cccttgggag gtggcggcat atgcaaagga tagcactccc actctactac
     5281 tgggtatcat atgctgactg tatatgcatg aggatagcat atgctacccg gatacagatt
     5341 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
     5401 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
     5461 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
     5521 aggatagcat atgctaccca gatatagatt aggatagcat atgctatcca gatatttggg
     5581 tagtatatgc tacccagata taaattagga tagcatatac taccctaatc tctattagga
     5641 tagcatatgc tacccggata cagattagga tagcatatac tacccagata tagattagga
     5701 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata taaattagga
     5761 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
     5821 tagcctatgc tacccagata tagattagga tagcatatgc tatccagata tttgggtagt
     5881 atatgctacc catggcaaca ttagcccacc gtgctctcag cgacctcgtg aatatgagga
     5941 ccaacaaccc tgtgcttggc gctcaggcgc aagtgtgtgt aatttgtcct ccagatcgca
     6001 gcaatcgcgc ccctatcttg gcccgcccac ctacttatgc aggtattccc cggggtgcca
     6061 ttagtggttt tgtgggcaag tggtttgacc gcagtggtta gcggggttac aatcagccaa
     6121 gttattacac ccttatttta cagtccaaaa ccgcagggcg gcgtgtgggg gctgacgcgt
     6181 gcccccactc cacaatttca aaaaaaagag tggccacttg tctttgttta tgggccccat
     6241 tggcgtggag ccccgtttaa ttttcggggg tgttagagac aaccagtgga gtccgctgct
     6301 gtcggcgtcc actctctttc cccttgttac aaatagagtg taacaacatg gttcacctgt
     6361 cttggtccct gcctgggaca catcttaata accccagtat catattgcac taggattatg
     6421 tgttgcccat agccataaat tcgtgtgaga tggacatcca gtctttacgg cttgtcccca
     6481 ccccatggat ttctattgtt aaagatattc agaatgtttc attcctacac tagtatttat
     6541 tgcccaaggg gtttgtgagg gttatattgg tgtcatagca caatgccacc actgaacccc
     6601 ccgtccaaat tttattctgg gggcgtcacc tgaaaccttg ttttcgagca cctcacatac
     6661 accttactgt tcacaactca gcagttattc tattagctaa acgaaggaga atgaagaagc
     6721 aggcgaagat tcaggagagt tcactgcccg ctccttgatc ttcagccact gcccttgtga
     6781 ctaaaatggt tcactaccct cgtggaatcc tgaccccatg taaataaaac cgtgacagct
     6841 catggggtgg gagatatcgc tgttccttag gaccctttta ctaaccctaa ttcgatagca
     6901 tatgcttccc gttgggtaac atatgctatt gaattagggt tagtctggat agtatatact
     6961 actacccggg aagcatatgc tacccgttta gggttaacaa gggggcctta taaacactat
     7021 tgctaatgcc ctcttgaggg tccgcttatc ggtagctaca caggcccctc tgattgacgt
     7081 tggtgtagcc tcccgtagtc ttcctgggcc cctgggaggt acatgtcccc cagcattggt
     7141 gtaagagctt cagccaagag ttacacataa aggcaatgtt gtgttgcagt ccacagactg
     7201 caaagtctgc tccaggatga aagccactca gtgttggcaa atgtgcacat ccatttataa
     7261 ggatgtcaac tacagtcaga gaaccccttt gtgtttggtc cccccccgtg tcacatgtgg
     7321 aacagggccc agttggcaag ttgtaccaac caactgaagg gattacatgc actgccccgc
     7381 gaagaagggg cagagatgtc gtagtcaggt ttagttcgtc cggggcgggg atctggccgc
     7441 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7501 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7561 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7621 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7681 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7741 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7801 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7861 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7921 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7981 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8041 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8101 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8161 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8221 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8281 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8341 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8401 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8461 aaagtgccac cagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa
     8521 agcatttgac ttgttctatg ccctaggggg cggggggaag ctaagccagc tttttttaac
     8581 atttaaaatg ttaattccat tttaaatgca cagatgtttt tatttcataa gggtttcaat
     8641 gtgcatgaat gctgcaatat tcctgttacc aaagctagta taaataaaaa tagataaacg
     8701 tggaaattac ttagagtttc tgtcattaac gtttccttcc tcagttgaca acataaatgc
     8761 gctgctgaga agccagtttg catctgtcag gatcaatttc ccattatgcc agtcatatta
     8821 attactagtc aattagttga tttttatttt tgacatatac atgtgaaaga ccccacctgt
     8881 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     8941 agaatagaaa agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
     9001 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     9061 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     9121 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     9181 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     9241 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     9301 ctcactcggc gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     9361 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     9421 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     9481 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt
     9541 gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag
     9601 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc
     9661 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc
     9721 aaaaatcccg atcgttttgg actctttggt gcacccccct tagaggaggg atatgtggtt
     9781 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     9841 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
     9901 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gccagactgt taccactccc
     9961 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
    10021 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
    10081 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
    10141 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
    10201 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
    10261 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
    10321 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatctta
    10381 tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac
    10441 agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga
    10501 cctctggcgg cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga
    10561 gtcggcgaca cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa
    10621 ggaccttaca cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct
    10681 tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg
    10741 ccggatccca gtgtggtggt acgggaattc gcggccgctc tagaaagctt gcaccatgga
    10801 tgtctctctt tgcccagcca agtgtagttt ctggcggatt ttcttgctgg gaagcgtctg
    10861 gctggactat gtgggctccg tgctgatggt gagcaagggc gaggagctgt tcaccggggt
    10921 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
    10981 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
    11041 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
    11101 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
    11161 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
    11221 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
    11281 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
    11341 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
    11401 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
    11461 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
    11521 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
    11581 cggcatggac gagctgtaca aggcttgccc tgcaaattgt gtctgcagca agactgagat
    11641 caattgccgg cggccggacg atgggaacct ctttcccctc ctggaagggc aggattcagg
    11701 gaacagcaat gggaacgcca gcatcaacat cacggacatc tcaaggaata tcacttccat
    11761 acacatagag aactggcggg gcctgcacac actcaatgct gtggatatgg agctctacac
    11821 gggactccag aagctgacca tcaagaattc tggactccgg aatattcagc ccagagcctt
    11881 tgctaagaat ccccacttgc gttatataaa cttgtcaagt aaccggctca ccacactctc
    11941 ctggcagctc ttccagacgc tgagtcttcg ggaattgaga ctggagcaga acttcttcaa
    12001 ctgcagctgt gacatccgct ggatgcagct gtggcaggaa cagggggagg cacggctgga
    12061 cagccagagc ctctactgca tcagtgctga tggctcccaa ctccccctct tccgcatgaa
    12121 catcagtcag tgtgatctcc ctgagatcag tgtgagtcac gtgaacctga ctgtccgaga
    12181 aggagacaat gctgtaatca cttgtaatgg ctctggctct cctttgcctg atgtggactg
    12241 gatagtcacc ggactgcagt ccatcaatac tcatcagacc aatctgaact ggaccaatgt
    12301 acatgccatc aacttgaccc tggtgaatgt gacgagcgaa gacaatggtt tcaccctgac
    12361 gtgcattgca gagaacgtgg tgggcatgag caatgccagc gttgctctca ctgtctacta
    12421 ccctccacgt gtggtgagct tggtggagcc tgaggtacgc ctggaacatt gcattgagtt
    12481 tgtggtgcgt ggcaacccga cacccacgct gcattggctg tacaatggac agccactgag
    12541 ggaatccaag atcattcata tggactacta ccaggagggt gaagtctcag agggctgcct
    12601 gctcttcaac aagcccaccc actacaacaa tggcaactac accctcattg ctaagaatgc
    12661 cctgggcaca gccaaccaga ccatcaacgg ccacttcctt aaggagccct ttccagagag
    12721 cacagatttc tttgactttg agtctgatgc gagccctaca cctcctatca ctgtgaccca
    12781 caaaccagag gaagacactt ttggggtgtc catagcagtt ggacttgctg cttttgcctg
    12841 cgtccttctg gtggtcctct ttatcatgat caacaagtac ggtcgacggt ccaaatttgg
    12901 aatgaagggt cctgtggctg ttatcagtgg ggaggaggac tcagccagcc cactgcacca
    12961 tatcaaccat ggcatcacta caccttcatc gctggatgct gggccggata cagtggtcat
    13021 tggcatgact cgaattccag tcattgagaa cccccaatat ttccgtcagg gacacaattg
    13081 ccacaagcca gacacatatg ttcaacacat caagaggaga gacatcgtgt tgaagagaga
    13141 actgggtgag ggagcctttg gaaaggtctt cctggctgag tgctacaatc taagccccac
    13201 caaagacaag atgcttgtgg cagtgaaggc cctgaaggat cccaccttgg ctgccaggaa
    13261 ggatttccag agggaggccg agctgctcac taacctgcag catgagcata ttgtcaaatt
    13321 ctatggggtc tgtggtgatg gggatccact catcatggtc tttgaataca tgaagcatgg
    13381 agacctgaac aagttcctca gggcccatgg gccagatgcg atgatccttg tggatggaca
    13441 gccacgccag gccaaggggg agctaggact ctcccagatg ctccacatcg ccagtcagat
    13501 agcctctggc atggtgtact tggcctccca gcactttgtg caccgggacc tggccaccag
    13561 gaactgccta gttggagcca accttctagt gaagattgga gattttggca tgtccaggga
    13621 cgtctacagt actgattact acagggtggg aggacacacc atgctcccca tccgctggat
    13681 gccacctgaa agcatcatgt accggaagtt taccacagag agtgatgtgt ggagctttgg
    13741 ggttattctt tgggagatct ttacctatgg gaagcaacca tggttccagc tctctaacac
    13801 agaggtcatt gaatgcatca cccaaggccg tgtcttggag agaccccgag tctgcccaaa
    13861 agaagtatat gatgtcatgc tggggtgctg gcagagggaa ccacaacagc gactgaatat
    13921 taaggagatc tacaaaatcc tccatgcttt ggggaaggcc accccaatct acctggacat
    13981 tcttggctag ctcgaggggc cgccagcaca gtggtcgacg ataaaataaa agattttatt
    14041 tagtct
//
