LOCUS       sequence-360266-        8333 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant,
            furin side replaced by GSAS, 2 Proline at 986 and 987 plus 
            T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Gobeil SM, Henderson R, Stalls V, Janowska K, Huang X, May A, 
            Speakman M, Beaudoin E, Manne K, Li D, Parks R, Barr M, Deyton M, 
            Martin M, Mansouri K, Edwards RJ, Eaton A, Montefiori DC, Sempowski 
            GD, Saunders KO, Wiehe K, Williams W, Korber B, Haynes BF, Acharya P
  TITLE     Structural diversity of the SARS-CoV-2 Omicron spike.
  JOURNAL   Mol Cell. 2022 Jun 2;82(11):2050-2068.e6. doi: 
            10.1016/j.molcel.2022.03.028. Epub 2022 Mar 25.
  PUBMED    35447081
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.03.028"; journalName: "Mol Cell"; date: 
            "2022-06-2- 2"; volume: "82"; issue: "11"; pages: "2050-2068.e6"
COMMENT     Sequence Label: p-alpha-H-S-GSAS-B.1.617.2.v1
FEATURES             Location/Qualifiers
     source          1..8333
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        119..498
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(634..702,703..1494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(852..871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1665..2253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2154..2173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2407..2424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2553..2574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2589..2594,2595..2612,2613..2619)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2627..2643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2632..2654
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2651..2667
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2651..2667
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3010..3029
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3028..3083
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3129..3148
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3250..3273)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(3313..3336)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(3343..3366)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(3370..3393)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     intron          7195..8211
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(7249..7271)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cccccccccc cgcccccatc gctgcacaaa ataattaaaa aataaataaa tacaaaattg
       61 ggggtgggga ggggggggag atggggagag tgaagcagaa cgtggggctc acctcgacca
      121 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
      181 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
      241 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
      301 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
      361 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
      421 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
      481 tagtcaataa tcaatgtcga cccaggtggc acttttcggg gaaatgtgcg cggaacccct
      541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      601 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      721 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      781 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      841 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      961 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1021 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1081 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1141 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1201 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1321 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1441 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1501 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2281 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2341 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2401 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2461 cccgcgcgtt ggccgattca ttaatgcagc ggatcgatcc gctggcacga caggtttccc
     2521 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2581 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2641 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgggct gcaggtcgag
     2701 ggatctccat aagagaagag ggacagctat gactgggagt agtcaggaga ggaggaaaaa
     2761 tctggctagt aaaacatgta aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa
     2821 acaaaatata aaaaaaatct aacctcaagt caaggctttt ctatggaata aggaatggac
     2881 agcagggggc tgtttcatat actgatgacc tctttatagc caacctttgt tcatggcagc
     2941 cagcatatgg gcatatgttg ccaaactcta aaccaaatac tcattctgat gttttaaatg
     3001 atttgccctc ccatatgtcc ttccgagtga gagacacaaa aaattccaac acactattgc
     3061 aatgaaaata aatttccttt attagccaga agtcagatgc tcaaggggct tcatgatgtc
     3121 cccataattt ttggcagagg gaaaaagatc tcagtggtat ttgtgagcca gggcattggc
     3181 cacaccagcc accaccttct gataggcagc ctgcacctga ggagtgaatt atcgctcgag
     3241 tcattatcac ttttcgaact gggggtggct ccaagctgag ccgccagatc cgccccctcc
     3301 actaccgccg cccttctcga actgggggtg ggaccaggcg ctatgatggt ggtgatggtg
     3361 gtgatggcct gggccctgga acagcacctc cagggatctg cccaggaatg tgctcagcag
     3421 tacccactcg ccgtccttcc gcacgtaggc ctggccatct ctgggggcct cggggatgta
     3481 tccggatccc tgctcgtatt tgccgagctc ctgcaggtcg atcagggact cgttcaggtt
     3541 cttggccacc tcgttcagtc tgtcgatttc cttttggatg ttcaccacgc tggcgttaat
     3601 gccactgatg tcgcccaggt ccacatcggg gctggtgtgg tttttaaagt acttgtccag
     3661 ctcttcctta aaagagtcca gctcaggttg cagagggtcg tacactgtgt tgttcacgat
     3721 tccgatgacc acgtcacaat tgccactcac gaaggtgttg tcggtggtga taatctgggg
     3781 ttcgtagaag tttctttgtg tcacgaacca gtgtgtgcca ttggaaacga aaacgccctc
     3841 tcttgggaag tgggccttgc cgtcgtggca gatggcaggg gcggttgtga aattcttctc
     3901 ttgggcaggc acgtatgtca catgcaggaa caccacgccg tgcggcgcgc tctgggggaa
     3961 gctcatcagg tgatagcctt tgccgcagaa gtcaacgcgt ttagactgtc ccagcacaca
     4021 ctcggacatc ttggtagcgg cgagattggc gctggctctt atttcagcgg ccctgatcag
     4081 ctgttgggtc acgtaggttt gcagggactg cagtctacct gtgatcagtc tatcgatctg
     4141 gacttcggct tccaccttgt ccagtctaga caggatatcg ttcagcacag agctgatggc
     4201 gccaaaattg ctgctgagct gcttcaccag ggtgttcagg gcttgggcgt tctgattcac
     4261 cacgttctgc agtttgccca gggcgctggc ggtagaggag agggagtcct ggatcttgcc
     4321 gatggcagaa ttgaactggt tagcgatcag cttctggttt tcatacagca cgttctgggt
     4381 cacgccgatt ccgttgaatc tataggccat ctgcatggca aaggggatct gcagggcggc
     4441 gccggcgcca aatgtccacc cagatgtgat ggtgccagcc agcagggcag aggtgtactg
     4501 agcgatcatc tcgtcggtca gcagaggtgg gagaactgtc aggccgttga atttctgggc
     4561 gcagatcaga tctctggcgg ctatgtcgcc caggcaatcg ccatactgtt tgataaagcc
     4621 ggcatcggcc agggtgacct tgttgaacag cagatcctcg ataaagctcc gcttgctagg
     4681 cttagaagga tcagggagaa tctggctaaa gttaaagccg ccgaagtcct tgataggtgg
     4741 ggtcttgtag atctgcttca cttgggcgaa cacctcctgg gtatttttgt cctgttccac
     4801 ggcgattccg gtcagcgccc ggttcagttg ggtgcaaaag gagccgtatt gcagcagcag
     4861 gttgctgcat tcagtgctgt cgccacagat gtacattgta cagtccacgc tggtcttggt
     4921 catgctcacg ggcaggatct ctgttgtgac gctaattgta aagttggtgg gaatggcgat
     4981 agagttgttg gaataggcca cgctgttctc tgcgcccagg ctcatggtgt aggcgatgat
     5041 gctctggcta gctacagagg aagcagaccc tctgctgttt gtctgggtct ggtagctggc
     5101 acagatgccg gcgccgatag ggatgtcgca ctcgtaactg ttattcacgt gctcggcgcc
     5161 gatcagacat ccggccctgg tctggaacac attggagcca gtggagtaca ctctccaagt
     5221 aggtgtcagc tgatcggcgt ggatggccac aggaacctcg gtgcagttaa caccttggta
     5281 cagcacagcc acctggttgc tggtattggt cccgggggtg atcacagaaa ctcctccgaa
     5341 ggagcagggt gtaatatcca ggatttccag ggtctgtgga tctctgacgg catctgtggt
     5401 gtcagcgata tcacggccga actgctggaa ggggaggaac tttttgttgc tttcggtcag
     5461 cacgccggtc ccagtcaggc cgttgaagtt gaagttcacg catttgttct taaccagatt
     5521 ggtggacttc ttaggaccgc agacggtggc gggggcgtgg agtaactcga aggacagcac
     5581 gaccacccgg tatggctggt agccgactcc attggtaggc tggaagccgt agctttgcag
     5641 agggaagtaa cagttgaagc cttccacccc gttacaaggc ttagaaccgg cctggtagat
     5701 ctctgtggag atgtctctct cgaaaggttt caggttagac ttgcgaaaca gccggtatct
     5761 gtagttgtag ttgccgccca ccttgctatc caggttgtta gagttccagg cgatcacgca
     5821 gcctgtaaaa tcatcaggca gcttatagtt gtagtcagcg atcttaccgg tctggcctgg
     5881 tgcgatctgc cgcacctcat cgcctctaat tacgaaggag tcggcgtaca cgttggtgaa
     5941 gcacaggtca ttcagctttg ttggggaaac gccgtagcac ttgaaggtgc tgaaagaggc
     6001 gctgttatac agcacggagt agtcggccac acaattgctg attctctttc tattccaggc
     6061 gtaaacggag gcaaatcttg tggcgttaaa cacctcgccg aaggggcaga ggttggtaat
     6121 gtttggaaat cgcacgatgc tctctgtagg ctgcacccgg aagttgctgg tctggtagat
     6181 gcccttttcc acggtgaagg acttcagggt gcactttgtc tcagagagag ggtccagggc
     6241 gcagtcgacg gcgtctgtga ttgtgccgtt ctcgttgtac ttcagcagga aggttctagg
     6301 ttgcaggtag cccacatagt aggctgcagc tccggcggtc catccgctgc tgctgtcgcc
     6361 aggagtcagg taggatctat gcagggccag cagggtttgg aaccgcgtta tgttgatgcc
     6421 tatgggcagg tcgaccaggg gttccagggc gctgaagccc tgagggaggt cccggaccag
     6481 gttgatgggt gtgtgcttgg agtagatttt gaagtagccg tcgatgttct tgaacacgaa
     6541 ttctctcagg ttcttgaagt tgccttgttt cccctcaagg tccatcagaa aaggctgaga
     6601 gacgtactcg aatgtgcagt tgttggcgct gctgtagacg ccgctttcca tccagctctt
     6661 gttgttcttg tggtagtaca cgtccaggaa aggatcattg caaaactgga actcgcacac
     6721 cttgatcacg acgttcgtgg cattgttcac gatcaggagg ctctgtgttt tgctatccag
     6781 ggtggtgccg aagatccagc cccggatgat gttactcttc tcggtactgg cgaagtaaac
     6841 tccatcattg aatggcagga cggggttatc aaaccgcttg gttccgtttg tgccgctcac
     6901 gtggatggcg tggaaccatg tcacgttgct gaagaacggc aggaagaggt cctgagtgct
     6961 gtgcaggacg ctagatctga acaccttgtc agggtagtac acgccccgtg taaagctgtt
     7021 ggtgtaggct ggtgggagct gggttcgggt tctcaggttc acgcactggc tgctcaccag
     7081 aggcaggagc accaggaaca cgaacatggt ggcggtaccg aactcggcct gagacggccg
     7141 caattctttg ccaaaatgat gagacagcac aacaaccagc acgttgccca ggagctgtag
     7201 gaaaaagaag aaggcatgaa catggttagc agaggcgcta gagccgccgg tcacacgcca
     7261 gaagccgaac cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc gcggacagcc
     7321 ccgaggctgg agagggagaa ggggacggcg gcgcggcgac gcacgaaggc cctccccgcc
     7381 catttccttc ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg gggtgcggcg
     7441 gcgcctccca gatttcggct ccgcacagat ttgggacaaa ggaagtccct gcgccctctc
     7501 gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc cgccggcgct
     7561 ccgggggccg ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc ccgccccgcc
     7621 cggcaccccc acctgccgcc accccccgcc cggcacggcg agccccgcgc cacgccccgt
     7681 acggagcccc gcacccgaag ccgggccgtg ctcagcaact cggggagggg ggtgcagggg
     7741 gggttgcagc ccgaccgacg cgcccacacc ccctgctcac ccccccacgc acacaccccg
     7801 cacgcagcct ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc ccccggccgc
     7861 gctcccctcg cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc agcgctcaca
     7921 gccgccgggc agcgcggagc cgcacgcggc gctccccacg cacacacaca cgcacgcacc
     7981 ccccgagccg ctccccccgc acaaagggcc ctcccggagc ccctcaaggc tttcacgcag
     8041 ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa ttacagcccg gaggagaagg
     8101 gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg
     8161 cgaggcggcg gcggagcggg gcacggggcg aaggcagcgc gcagcgactc ccgcccgccg
     8221 cgcgcttcgc tttttatagg gccgccgccg ccgccgcctc gccataaaag gaaactttcg
     8281 gagcgcgccg ctctgattgg ctgccgccgc acctctccgc ctcgccccgs cnc
//
